Reviewed, UniProtKB/Swiss-Prot B8CUG6 (TPMT_SHEPW)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	swp_4515

Organism

Shewanella piezotolerans (strain WP3 / JCM 13877) [Complete proteome] [HAMAP]

Taxonomic identifier

225849 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000134080
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	126	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8CUG6-1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: CAE1E28D1DA15C20	FASTA	222	25,039
	10 20 30 40 50 60 MQPSFWHEKW DAQQIGFHLS AVNPLLIQFW SQLKLPANSQ VFVPLCGKSL DMCFLAEQGH 70 80 90 100 110 120 DVLACELNDL AVSQFYQENN LTHTVEKVGE HKRYSTEQIT IYQGDIFSLQ NTNSVELAKT 130 140 150 160 170 180 SAFYDRAALI AWPEAMRLQY AQQLACLIPA GSVGLLVTLD YPQAELNGPP FSVSDDWMQA 190 200 210 220 NMGADFEIER LACEDVLSDN PRFVKKQVSS LTESVYKLTR KG

References

[1]

"Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3." Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X. PLoS ONE 3:E1937-E1937(2008) [PubMed: 18398463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000472 Genomic DNA. Translation: ACJ31158.1.
RefSeq	YP_002313745.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7035443.
GenomeReviews	Gene locus swp_4515 in contig CP000472_GR.
KEGG	swp:swp_4515.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_SHEPW

Accession

Primary (citable) accession number: B8CUG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	June 16, 2009
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)