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B8CSC1

- SYI_SHEPW

UniProt

B8CSC1 - SYI_SHEPW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella piezotolerans (strain WP3 / JCM 13877)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSPIE225849:GH6V-3628-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:swp_3728
    OrganismiShewanella piezotolerans (strain WP3 / JCM 13877)
    Taxonomic identifieri225849 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000000753: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000189195Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi225849.swp_3728.

    Structurei

    3D structure databases

    ProteinModelPortaliB8CSC1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8CSC1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKSTLNL PETEFPMRGN LANREPEMLK SWNEKGLYQQ IRESRKDSKP    50
    FILHDGPPYA NGDIHIGHSV NKILKDIIIK SKTMSGFDAP YIPGWDCHGL 100
    PIELKVEQKV GKPGHKVTAS EFRQKCREYA AKQVDGQRDD FIRLGVFADW 150
    QNPYLTMDYN TEANIVRSLS KVIDSGHLHK GVKPVHWCTD CGSALAEAEV 200
    EYEDKMSPAI DVAFTAVDKP ALLAKFGLTE YAHPISMVIW TTTPWTLPAN 250
    RALSVAGQLE YTLVEMVKDG QTQALVLAQE LVESCVERFG AETHKVLANV 300
    LGSELELLRF NHPFYDFDVP VILGEHVTVE SGTGVVHTAP GHGQDDFVVG 350
    QKYGLEVANP VGDNGVYKAD TEIFAGQHVF KANKAVVALL EEKGALLNHV 400
    AINHSYPHCW RHKTPIIFRA TPQWFISMDQ KGLRTQALSE IEKTQWIPDW 450
    GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRETEELHP DSVSLMERVA 500
    NRIEQEGIQA WWDLDAAELL GDEAEQYRKV TDTLDVWYDS GSTFSSVVAS 550
    RPEFNGHEVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
    GKGRKMSKSV GNVIAPQHVT NKLGADILRL WVAATDYSGE MTVSDEILKR 650
    SADAYRRIRN TARFLLANIN GFNPETDMVA VEDMVALDRW VVRRAAALQQ 700
    ELLEAYDQYN FHLVTQKLMQ FCSVELGSFY LDIIKDRQYT AKDDSNARRS 750
    CQSALYLISE AMVRWIAPVL SFTADEIWKL LPGERGEFVF TETWYEGLQS 800
    VTLDSDLSDE FWEQLLTVRA EVNKVIENAR REKQIGGSLE AEIMLFADEA 850
    LSTALSTLGD ELRFVLLTSK TDVVALSEAP ADAIETELAS LKLGLKKSEA 900
    EKCERCWHHR EEVGQVAEHP TLCTRCVTNI EGEGETRQFA 940
    Length:940
    Mass (Da):105,982
    Last modified:March 3, 2009 - v1
    Checksum:iF23DFCF6DF1F28A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000472 Genomic DNA. Translation: ACJ30411.1.
    RefSeqiYP_002312998.1. NC_011566.1.

    Genome annotation databases

    EnsemblBacteriaiACJ30411; ACJ30411; swp_3728.
    GeneIDi7034696.
    KEGGiswp:swp_3728.
    PATRICi23545829. VBIShePie65056_3425.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000472 Genomic DNA. Translation: ACJ30411.1 .
    RefSeqi YP_002312998.1. NC_011566.1.

    3D structure databases

    ProteinModelPortali B8CSC1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 225849.swp_3728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACJ30411 ; ACJ30411 ; swp_3728 .
    GeneIDi 7034696.
    KEGGi swp:swp_3728.
    PATRICi 23545829. VBIShePie65056_3425.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SPIE225849:GH6V-3628-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3."
      Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X.
      PLoS ONE 3:E1937-E1937(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WP3 / JCM 13877.

    Entry informationi

    Entry nameiSYI_SHEPW
    AccessioniPrimary (citable) accession number: B8CSC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3