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B8CQY2 (BIOB_SHEPW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:swp_2928
OrganismShewanella piezotolerans (strain WP3 / JCM 13877) [Complete proteome] [HAMAP]
Taxonomic identifier225849 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350Biotin synthase HAMAP-Rule MF_01694
PRO_0000381624

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2631Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B8CQY2 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 9E6BF7B78E849BBF

FASTA35038,889
        10         20         30         40         50         60 
MSDVQVRNNW KRDEIETLFA LPMNDLLFQA HSVHRQEFDP NEVQISRLLS IKTGACPEDC 

        70         80         90        100        110        120 
KYCPQSARYD TGLEKERLLA METVLTEARS AKAAGASRFC MGAAWRNPKE RDMPYLKTMV 

       130        140        150        160        170        180 
EEVKSLGMET CMTLGMLSAD QASELADAGL DYYNHNLDTS PEYYGDVITT RTYQNRLDTL 

       190        200        210        220        230        240 
SNVRASGMKV CSGGIVGMGE KATDRAGLLQ QLANLDKHPD SVPINMLVKV EGTPFEKIDD 

       250        260        270        280        290        300 
LDPLEFVRTI AVARIIMPKS RVRLSAGREN MTDELQAMCF FAGANSIFYG CKLLTTPNPE 

       310        320        330        340        350 
ENDDMSLFRR LGLHPEQGIA ATKEQDEAML AKAAAQQDKK ASAFYDAGAL 

« Hide

References

[1]"Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3."
Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X.
PLoS ONE 3:E1937-E1937(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WP3 / JCM 13877.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000472 Genomic DNA. Translation: ACJ29654.1.
RefSeqYP_002312241.1. NC_011566.1.

3D structure databases

ProteinModelPortalB8CQY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING225849.swp_2928.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ29654; ACJ29654; swp_2928.
GeneID7033939.
KEGGswp:swp_2928.
PATRIC23544360. VBIShePie65056_2696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycSPIE225849:GH6V-2870-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SHEPW
AccessionPrimary (citable) accession number: B8CQY2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways