B8CQJ5 (RNC_SHEPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	swp_3780

Organism

Shewanella piezotolerans (strain WP3 / JCM 13877) [Complete proteome] [HAMAP]

Taxonomic identifier

225849 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella piezotolerans

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000194431
Regions
	Domain	7 – 129	123	RNase III
	Domain	155 – 225	71	DRBM
Sites
	Active site	46	1	Potential
	Active site	118	1	By similarity
	Metal binding	42	1	Magnesium By similarity
	Metal binding	115	1	Magnesium By similarity
	Metal binding	118	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8CQJ5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 13A98EFEE2EED3F5	FASTA	225	25,326
	10 20 30 40 50 60 MEPIKNIPRL CRTLGYEFAE QALLDQALTH RSASNKHNER LEFLGDSILS IVISDALYHQ 70 80 90 100 110 120 FPKATEGDLS RMRATLVCGK MLAEIAIEFK LGDYLKLGPG ELKSGGFRRE SILADAVEAI 130 140 150 160 170 180 IGAIYLDSDI EKCRKLVLNW YASRLRVIEP INQKDPKTLL QEHLQKFRKP LPIYKVVHTE 190 200 210 220 GDAHEQTFTV ECVVEDLRQA VVGVASSRRK AEQSAAAQVL ELIKK

References

[1]

"Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3." Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X. PLoS ONE 3:E1937-E1937(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WP3 / JCM 13877.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000472 Genomic DNA. Translation: ACJ30461.1.
RefSeq	YP_002313048.1. NC_011566.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	225849.swp_3780.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ30461; ACJ30461; swp_3780.
GeneID	7034746.
KEGG	swp:swp_3780.
PATRIC	23545925. VBIShePie65056_3473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246809.
KO	K03685.
OMA	RACELPR.
ProtClustDB	PRK00102.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_SHEPW

Accession

Primary (citable) accession number: B8CQJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program