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Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Shewanella piezotolerans (strain WP3 / JCM 13877)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei676ATP; via carbonyl oxygenUniRule annotation1
Metal bindingi677MagnesiumUniRule annotation1
Metal bindingi716MagnesiumUniRule annotation1
Metal bindingi720MagnesiumUniRule annotation1
Metal bindingi884MagnesiumUniRule annotation1
Binding sitei886ATPUniRule annotation1
Active sitei1133NucleophileUniRule annotation1
Active sitei1258UniRule annotation1
Active sitei1260UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi305 – 316ATPUniRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processPurine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPIE225849:G1G7F-1324-MONOMER
UniPathwayiUPA00074; UER00128

Protein family/group databases

MEROPSiC56.972

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Short name:
FGAMSUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
Short name:
FGAR amidotransferaseUniRule annotation
Short name:
FGAR-ATUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:swp_1467Imported
OrganismiShewanella piezotolerans (strain WP3 / JCM 13877)Imported
Taxonomic identifieri225849 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000000753 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi225849.swp_1467

Structurei

3D structure databases

ProteinModelPortaliB8CKS7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1040 – 1293Glutamine amidotransferase type-1InterPro annotationAdd BLAST254

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili179 – 199Sequence analysisAdd BLAST21

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis, Glutamine amidotransferaseUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4108ETH Bacteria
COG0046 LUCA
COG0047 LUCA
HOGENOMiHOG000261359
KOiK01952
OMAiSLSANWM
OrthoDBiPOG091H021O

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.40.50.880, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00419 PurL_1, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010073 PRibForGlyAmidine_synth
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR036921 PurM-like_N_sf
IPR036604 PurS-like_sf
PANTHERiPTHR10099:SF5 PTHR10099:SF5, 1 hit
PfamiView protein in Pfam
PF02769 AIRS_C, 2 hits
SUPFAMiSSF52317 SSF52317, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
SSF82697 SSF82697, 1 hit
TIGRFAMsiTIGR01735 FGAM_synt, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

B8CKS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIIRGAPAL SAFRVQKLMQ ACESAALPVR QIYAEFIHLA DLKESLEENE
60 70 80 90 100
SQQLEKILTY GPAIEAHQPE GKLHFVTPRP GTISPWSSKA TDIAHNCGLD
110 120 130 140 150
KVLRLERGIA YYVVADELTA AQTKSLLELL HDRMVEVVVN DFNAADVLFA
160 170 180 190 200
RTEPAKFKSV DILGQGRSAL ELANTTLGLA LAEDEIDYLV ENFKRLERNP
210 220 230 240 250
NDVELMMFAQ ANSEHCRHKI FNADWTIDGE VQPKSLFKMI KNTFETTPEN
260 270 280 290 300
VLSAYKDNAA VMTGSTAGRF FPKQDGVYGY HTEPMHILMK VETHNHPTAI
310 320 330 340 350
SPYPGAATGS GGEIRDEGAT GRGSKPKAGL TGFSVSNLKI PGFVQPWEAD
360 370 380 390 400
YGKPDRIVTA LDIMTEGPLG GAAFNNEFGR PALVGYFRTY EQEVNSHNGV
410 420 430 440 450
EVRGYHKPIM LAGGLGNIRE EHVQKGEITV GAKLVVLGGP AMNIGLGGGA
460 470 480 490 500
ASSMTSGQSS EDLDFASVQR ENPEMERRCQ EVIDRCWQMG DANPIQFIHD
510 520 530 540 550
VGAGGLSNAF PELVDDGGRG GNFELRRVPS DEPGMSPLEI WCNESQERYV
560 570 580 590 600
MSIAPENLAV FTAICQRERA PFAVVGVATE ERELILSDEH FENHPIELPL
610 620 630 640 650
EVLLGKAPKM SRDVVSAKAN SSALDQSDIA VKEAAHRLLR LPTIAEKTFL
660 670 680 690 700
ITIGDRTVTG LVNRDQMVGP WQVPVADCAV TAASYDSYVG EAMSIGERTP
710 720 730 740 750
LALLDFGASA RMAVAESILN IAGTDIGSFK RIKLSANWMS PAGHPGEDAG
760 770 780 790 800
LYEAVKAIGE ELCPDLSLTI PVGKDSMSMK TAWEDNGVQK AVTSPMSLVI
810 820 830 840 850
TAFGVVQDVR NTVTPELRSD KGDSELLLLD LGLGQNRLGG SCLAQVYSEL
860 870 880 890 900
GDIAPDLDNS ATLKGFFEVI QPLVADQSIL AYHDRSDGGL YTTLVEMAFA
910 920 930 940 950
GHTGLNVDLS ALTGTDVERL FNEELGAVIQ VRREDSAAIS AKFNAAGVAC
960 970 980 990 1000
HKVAELTDTD QIAIFDGERE VLTETRTSLR TIWAETTYRM QSLRDNPECA
1010 1020 1030 1040 1050
KEEHALKQDA NDLGLTVDLS FDPSEDVAAP FILKGAAPKM AILREQGVNS
1060 1070 1080 1090 1100
HIEMAAAFDR AGFESKDVHM SDILSGRISL EEFQGLVACG GFSYGDVLGA
1110 1120 1130 1140 1150
GEGWAKSILF NARAREQFSQ FFERDDSFSL GVCNGCQMLS NLKDIIPGTD
1160 1170 1180 1190 1200
LWPHFVRNRS ERFEARFSLV EVQKSPSLFF QGMEGSRMPI AVSHGEGRAE
1210 1220 1230 1240 1250
FASPEALAAA EASGTIALRY VDGNGQIATQ YPQNPNGSPN AITALSSTDG
1260 1270 1280 1290
RVTIMMPHPE RVFRTVANSW HPDSWGEDSP WMRMFRNVRK SIG
Length:1,293
Mass (Da):140,704
Last modified:March 3, 2009 - v1
Checksum:i43C61359F178E86F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000472 Genomic DNA Translation: ACJ28253.1
RefSeqiWP_020911631.1, NC_011566.1

Genome annotation databases

EnsemblBacteriaiACJ28253; ACJ28253; swp_1467
KEGGiswp:swp_1467

Similar proteinsi

Entry informationi

Entry nameiB8CKS7_SHEPW
AccessioniPrimary (citable) accession number: B8CKS7
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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