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B8CKS7

- B8CKS7_SHEPW

UniProt

B8CKS7 - B8CKS7_SHEPW

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Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Shewanella piezotolerans (strain WP3 / JCM 13877)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei676 – 6761ATP; via carbonyl oxygenUniRule annotation
Metal bindingi677 – 6771MagnesiumUniRule annotation
Metal bindingi716 – 7161MagnesiumUniRule annotation
Metal bindingi720 – 7201MagnesiumUniRule annotation
Metal bindingi884 – 8841MagnesiumUniRule annotation
Binding sitei886 – 8861ATPUniRule annotation
Active sitei1133 – 11331NucleophileUniRule annotation
Active sitei1258 – 12581UniRule annotation
Active sitei1260 – 12601UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 31612ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPIE225849:GH6V-1415-MONOMER.
UniPathwayiUPA00074; UER00128.

Protein family/group databases

MEROPSiC56.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthaseUniRule annotationSAAS annotation (EC:6.3.5.3UniRule annotationSAAS annotation)
Short name:
FGAM synthaseUniRule annotation
Short name:
FGAMSUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:swp_1467Imported
OrganismiShewanella piezotolerans (strain WP3 / JCM 13877)Imported
Taxonomic identifieri225849 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000753: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi225849.swp_1467.

Structurei

3D structure databases

ProteinModelPortaliB8CKS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
Contains glutamine amidotransferase type-1 domain.SAAS annotation
Contains glutamine amidotransferase type-domain.SAAS annotation
In the N-terminal section; belongs to the FGAMS family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000261359.
KOiK01952.
OMAiGEGHAEF.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8CKS7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEIIRGAPAL SAFRVQKLMQ ACESAALPVR QIYAEFIHLA DLKESLEENE
60 70 80 90 100
SQQLEKILTY GPAIEAHQPE GKLHFVTPRP GTISPWSSKA TDIAHNCGLD
110 120 130 140 150
KVLRLERGIA YYVVADELTA AQTKSLLELL HDRMVEVVVN DFNAADVLFA
160 170 180 190 200
RTEPAKFKSV DILGQGRSAL ELANTTLGLA LAEDEIDYLV ENFKRLERNP
210 220 230 240 250
NDVELMMFAQ ANSEHCRHKI FNADWTIDGE VQPKSLFKMI KNTFETTPEN
260 270 280 290 300
VLSAYKDNAA VMTGSTAGRF FPKQDGVYGY HTEPMHILMK VETHNHPTAI
310 320 330 340 350
SPYPGAATGS GGEIRDEGAT GRGSKPKAGL TGFSVSNLKI PGFVQPWEAD
360 370 380 390 400
YGKPDRIVTA LDIMTEGPLG GAAFNNEFGR PALVGYFRTY EQEVNSHNGV
410 420 430 440 450
EVRGYHKPIM LAGGLGNIRE EHVQKGEITV GAKLVVLGGP AMNIGLGGGA
460 470 480 490 500
ASSMTSGQSS EDLDFASVQR ENPEMERRCQ EVIDRCWQMG DANPIQFIHD
510 520 530 540 550
VGAGGLSNAF PELVDDGGRG GNFELRRVPS DEPGMSPLEI WCNESQERYV
560 570 580 590 600
MSIAPENLAV FTAICQRERA PFAVVGVATE ERELILSDEH FENHPIELPL
610 620 630 640 650
EVLLGKAPKM SRDVVSAKAN SSALDQSDIA VKEAAHRLLR LPTIAEKTFL
660 670 680 690 700
ITIGDRTVTG LVNRDQMVGP WQVPVADCAV TAASYDSYVG EAMSIGERTP
710 720 730 740 750
LALLDFGASA RMAVAESILN IAGTDIGSFK RIKLSANWMS PAGHPGEDAG
760 770 780 790 800
LYEAVKAIGE ELCPDLSLTI PVGKDSMSMK TAWEDNGVQK AVTSPMSLVI
810 820 830 840 850
TAFGVVQDVR NTVTPELRSD KGDSELLLLD LGLGQNRLGG SCLAQVYSEL
860 870 880 890 900
GDIAPDLDNS ATLKGFFEVI QPLVADQSIL AYHDRSDGGL YTTLVEMAFA
910 920 930 940 950
GHTGLNVDLS ALTGTDVERL FNEELGAVIQ VRREDSAAIS AKFNAAGVAC
960 970 980 990 1000
HKVAELTDTD QIAIFDGERE VLTETRTSLR TIWAETTYRM QSLRDNPECA
1010 1020 1030 1040 1050
KEEHALKQDA NDLGLTVDLS FDPSEDVAAP FILKGAAPKM AILREQGVNS
1060 1070 1080 1090 1100
HIEMAAAFDR AGFESKDVHM SDILSGRISL EEFQGLVACG GFSYGDVLGA
1110 1120 1130 1140 1150
GEGWAKSILF NARAREQFSQ FFERDDSFSL GVCNGCQMLS NLKDIIPGTD
1160 1170 1180 1190 1200
LWPHFVRNRS ERFEARFSLV EVQKSPSLFF QGMEGSRMPI AVSHGEGRAE
1210 1220 1230 1240 1250
FASPEALAAA EASGTIALRY VDGNGQIATQ YPQNPNGSPN AITALSSTDG
1260 1270 1280 1290
RVTIMMPHPE RVFRTVANSW HPDSWGEDSP WMRMFRNVRK SIG
Length:1,293
Mass (Da):140,704
Last modified:March 3, 2009 - v1
Checksum:i43C61359F178E86F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000472 Genomic DNA. Translation: ACJ28253.1.
RefSeqiYP_002310840.1. NC_011566.1.

Genome annotation databases

EnsemblBacteriaiACJ28253; ACJ28253; swp_1467.
GeneIDi7032538.
KEGGiswp:swp_1467.
PATRICi23541531. VBIShePie65056_1341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000472 Genomic DNA. Translation: ACJ28253.1 .
RefSeqi YP_002310840.1. NC_011566.1.

3D structure databases

ProteinModelPortali B8CKS7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 225849.swp_1467.

Protein family/group databases

MEROPSi C56.972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ28253 ; ACJ28253 ; swp_1467 .
GeneIDi 7032538.
KEGGi swp:swp_1467.
PATRICi 23541531. VBIShePie65056_1341.

Phylogenomic databases

eggNOGi COG0046.
HOGENOMi HOG000261359.
KOi K01952.
OMAi GEGHAEF.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci SPIE225849:GH6V-1415-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPi MF_00419. PurL_1.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01735. FGAM_synt. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3."
    Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X.
    PLoS ONE 3:E1937-E1937(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WP3 / JCM 13877Imported.

Entry informationi

Entry nameiB8CKS7_SHEPW
AccessioniPrimary (citable) accession number: B8CKS7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3