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B8CKS7

- B8CKS7_SHEPW

UniProt

B8CKS7 - B8CKS7_SHEPW

Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Shewanella piezotolerans (strain WP3 / JCM 13877)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei676 – 6761ATP; via carbonyl oxygenUniRule annotation
    Metal bindingi677 – 6771MagnesiumUniRule annotation
    Metal bindingi716 – 7161MagnesiumUniRule annotation
    Metal bindingi720 – 7201MagnesiumUniRule annotation
    Metal bindingi884 – 8841MagnesiumUniRule annotation
    Binding sitei886 – 8861ATPUniRule annotation
    Active sitei1133 – 11331NucleophileUniRule annotation
    Active sitei1258 – 12581UniRule annotation
    Active sitei1260 – 12601UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi305 – 31612ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Purine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSPIE225849:GH6V-1415-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Protein family/group databases

    MEROPSiC56.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Short name:
    FGAMSUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
    Gene namesi
    Name:purLUniRule annotation
    Ordered Locus Names:swp_1467Imported
    OrganismiShewanella piezotolerans (strain WP3 / JCM 13877)Imported
    Taxonomic identifieri225849 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000000753: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi225849.swp_1467.

    Structurei

    3D structure databases

    ProteinModelPortaliB8CKS7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
    Contains glutamine amidotransferase type-1 domain.SAAS annotation
    In the N-terminal section; belongs to the FGAMS family.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferaseUniRule annotationSAAS annotation

    Phylogenomic databases

    eggNOGiCOG0046.
    HOGENOMiHOG000261359.
    KOiK01952.
    OMAiGEGHAEF.
    OrthoDBiEOG6FNHHR.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPiMF_00419. PurL_1.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010073. PRibForGlyAmidine_synth.
    IPR022940. PRibForGlyAmidine_synth_bac.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8CKS7-1 [UniParc]FASTAAdd to Basket

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    MEIIRGAPAL SAFRVQKLMQ ACESAALPVR QIYAEFIHLA DLKESLEENE     50
    SQQLEKILTY GPAIEAHQPE GKLHFVTPRP GTISPWSSKA TDIAHNCGLD 100
    KVLRLERGIA YYVVADELTA AQTKSLLELL HDRMVEVVVN DFNAADVLFA 150
    RTEPAKFKSV DILGQGRSAL ELANTTLGLA LAEDEIDYLV ENFKRLERNP 200
    NDVELMMFAQ ANSEHCRHKI FNADWTIDGE VQPKSLFKMI KNTFETTPEN 250
    VLSAYKDNAA VMTGSTAGRF FPKQDGVYGY HTEPMHILMK VETHNHPTAI 300
    SPYPGAATGS GGEIRDEGAT GRGSKPKAGL TGFSVSNLKI PGFVQPWEAD 350
    YGKPDRIVTA LDIMTEGPLG GAAFNNEFGR PALVGYFRTY EQEVNSHNGV 400
    EVRGYHKPIM LAGGLGNIRE EHVQKGEITV GAKLVVLGGP AMNIGLGGGA 450
    ASSMTSGQSS EDLDFASVQR ENPEMERRCQ EVIDRCWQMG DANPIQFIHD 500
    VGAGGLSNAF PELVDDGGRG GNFELRRVPS DEPGMSPLEI WCNESQERYV 550
    MSIAPENLAV FTAICQRERA PFAVVGVATE ERELILSDEH FENHPIELPL 600
    EVLLGKAPKM SRDVVSAKAN SSALDQSDIA VKEAAHRLLR LPTIAEKTFL 650
    ITIGDRTVTG LVNRDQMVGP WQVPVADCAV TAASYDSYVG EAMSIGERTP 700
    LALLDFGASA RMAVAESILN IAGTDIGSFK RIKLSANWMS PAGHPGEDAG 750
    LYEAVKAIGE ELCPDLSLTI PVGKDSMSMK TAWEDNGVQK AVTSPMSLVI 800
    TAFGVVQDVR NTVTPELRSD KGDSELLLLD LGLGQNRLGG SCLAQVYSEL 850
    GDIAPDLDNS ATLKGFFEVI QPLVADQSIL AYHDRSDGGL YTTLVEMAFA 900
    GHTGLNVDLS ALTGTDVERL FNEELGAVIQ VRREDSAAIS AKFNAAGVAC 950
    HKVAELTDTD QIAIFDGERE VLTETRTSLR TIWAETTYRM QSLRDNPECA 1000
    KEEHALKQDA NDLGLTVDLS FDPSEDVAAP FILKGAAPKM AILREQGVNS 1050
    HIEMAAAFDR AGFESKDVHM SDILSGRISL EEFQGLVACG GFSYGDVLGA 1100
    GEGWAKSILF NARAREQFSQ FFERDDSFSL GVCNGCQMLS NLKDIIPGTD 1150
    LWPHFVRNRS ERFEARFSLV EVQKSPSLFF QGMEGSRMPI AVSHGEGRAE 1200
    FASPEALAAA EASGTIALRY VDGNGQIATQ YPQNPNGSPN AITALSSTDG 1250
    RVTIMMPHPE RVFRTVANSW HPDSWGEDSP WMRMFRNVRK SIG 1293
    Length:1,293
    Mass (Da):140,704
    Last modified:March 3, 2009 - v1
    Checksum:i43C61359F178E86F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000472 Genomic DNA. Translation: ACJ28253.1.
    RefSeqiYP_002310840.1. NC_011566.1.

    Genome annotation databases

    EnsemblBacteriaiACJ28253; ACJ28253; swp_1467.
    GeneIDi7032538.
    KEGGiswp:swp_1467.
    PATRICi23541531. VBIShePie65056_1341.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000472 Genomic DNA. Translation: ACJ28253.1 .
    RefSeqi YP_002310840.1. NC_011566.1.

    3D structure databases

    ProteinModelPortali B8CKS7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 225849.swp_1467.

    Protein family/group databases

    MEROPSi C56.972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACJ28253 ; ACJ28253 ; swp_1467 .
    GeneIDi 7032538.
    KEGGi swp:swp_1467.
    PATRICi 23541531. VBIShePie65056_1341.

    Phylogenomic databases

    eggNOGi COG0046.
    HOGENOMi HOG000261359.
    KOi K01952.
    OMAi GEGHAEF.
    OrthoDBi EOG6FNHHR.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .
    BioCyci SPIE225849:GH6V-1415-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPi MF_00419. PurL_1.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010073. PRibForGlyAmidine_synth.
    IPR022940. PRibForGlyAmidine_synth_bac.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR01735. FGAM_synt. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3."
      Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X.
      PLoS ONE 3:E1937-E1937(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WP3 / JCM 13877Imported.

    Entry informationi

    Entry nameiB8CKS7_SHEPW
    AccessioniPrimary (citable) accession number: B8CKS7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 3, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3