Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO4

Gene

GLO4

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation (By similarity).By similarity

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstratePROSITE-ProRule annotation1
Binding sitei109FMNPROSITE-ProRule annotation1
Binding sitei130FMNPROSITE-ProRule annotation1
Binding sitei132SubstratePROSITE-ProRule annotation1
Binding sitei158FMNPROSITE-ProRule annotation1
Binding sitei167SubstratePROSITE-ProRule annotation1
Binding sitei231FMNPROSITE-ProRule annotation1
Active sitei255Proton acceptorPROSITE-ProRule annotation1
Binding sitei258SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 310FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Host-virus interaction, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 4
Short name:
GOX 4
Short name:
OsGLO4
Short chain alpha-hydroxy acid oxidase GLO4
Gene namesi
Name:GLO4
ORF Names:OsI_26871
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000007015 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004034161 – 366Peroxisomal (S)-2-hydroxy-acid oxidase GLO4Add BLAST366

Interactioni

Subunit structurei

Homotetramer or homooctamer. Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes (By similarity).By similarity

Protein-protein interaction databases

STRINGi39946.BGIOSGA026138-PA.

Structurei

3D structure databases

ProteinModelPortaliB8B8K5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 360FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST360

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi364 – 366Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
HOGENOMiHOG000217463.
OrthoDBiEOG09360EDE.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8B8K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDNLPVNVR EYQELAKKAL PKMAYDYING GAEDEHTLRE NIAAYTRIIL
60 70 80 90 100
RPRVLVDVSK IDMSTTLLGY TMRSPIIVAP TGGHKLAHPE GEKATARAAA
110 120 130 140 150
SCNAIMVLSF SSSCKIEDVA SSCNAIRFYQ LYVYKNRNVS ATLVRRAESC
160 170 180 190 200
GFKALLLTVD TPMLGRREAD IRNKMVFPRS GNLEGLMTID DHDTTNGSQL
210 220 230 240 250
ERFARATLDP SLSWKDIEWL KSITSMPIFL KGIVTAEDAR RAVEAGVAGV
260 270 280 290 300
IVSNHGARQL DYAPATIAAL EEVVRAVAGA VPVLVDGGIR RGTDVFKALA
310 320 330 340 350
LGARAVMXXX PVFFGLAARG EAGARHVIEM LNGELEVAMA LCGCRSVGEI
360
TRSHVMTEGD RIRSLL
Length:366
Mass (Da):39,798
Last modified:January 11, 2011 - v2
Checksum:iA4E02D01DB2ECED1
GO

Sequence cautioni

The sequence EEC82445 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000132 Genomic DNA. Translation: EEC82445.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000132 Genomic DNA. Translation: EEC82445.1. Sequence problems.

3D structure databases

ProteinModelPortaliB8B8K5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39946.BGIOSGA026138-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
HOGENOMiHOG000217463.
OrthoDBiEOG09360EDE.

Enzyme and pathway databases

UniPathwayiUPA00951; UER00912.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLO4_ORYSI
AccessioniPrimary (citable) accession number: B8B8K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: September 7, 2016
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.