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B8B6G5 (POED2_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyprenol reductase 2

EC=1.3.1.94
Gene names
ORF Names:OsI_26070
OrganismOryza sativa subsp. indica (Rice)
Taxonomic identifier39946 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism By similarity.

Catalytic activity

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Sequence caution

The sequence EEC82076.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EEC82076.1 differs from that shown. Reason: Frameshift at position 319.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Polyprenol reductase 2
PRO_0000398660

Regions

Transmembrane11 – 3121Helical; Potential
Transmembrane78 – 9821Helical; Potential
Transmembrane175 – 19521Helical; Potential
Transmembrane234 – 25421Helical; Potential
Transmembrane291 – 30818Helical; Potential
Transmembrane313 – 33523Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
B8B6G5 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 08FAD5C4D509AFA0

FASTA35339,903
        10         20         30         40         50         60 
MESEGWPALQ PLLCFAWIAA TLPIIAAALP IPTAVGGHLL RRLLSAFSSR GKTVRPSPAS 

        70         80         90        100        110        120 
SSGSSSSKAK FTVPQKYFMH FYVVGVLATT ILLLAIWFYA YMKLTPLLLE SSSYSTIFSH 

       130        140        150        160        170        180 
LVGSNSFSFG RVRSRTMGHK YRVWRTVFAL LLMEVQVLRR LYETEHVFHY SPARMHIVGY 

       190        200        210        220        230        240 
LTGLFYYVAA PLSLASSCIP EAAEYFQGQV PEFVVKGRAR MPDLVIDSSS LLQPLLKLGW 

       250        260        270        280        290        300 
TQWIGAVIFI WGSLHQIRCH AILGSLREHK DSDEYVIPCS DCFNRVSCPH YLAELVIYFG 

       310        320        330        340        350 
MLVASGAEDI PVWFLFIFLI TNLSFAAVET YNWYLQKFED YPRSRYAIIP FVC 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000132 Genomic DNA. Translation: EEC82076.1. Sequence problems.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os07g31140.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneB8B6G5.

Phylogenomic databases

HOGENOMHOG000240963.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOED2_ORYSI
AccessionPrimary (citable) accession number: B8B6G5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: March 19, 2014
This is version 19 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways