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B8B016 (LISC2_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase 2, chloroplastic

EC=2.8.1.8
Alternative name(s):
Lipoate synthase 2
Short name=LS 2
Short name=Lip-syn 2
Lipoate synthase, plastidial 2
Short name=LIP1p 2
Lipoic acid synthase 2
Gene names
Name:LIP1P-2
ORF Names:OsI_20590
OrganismOryza sativa subsp. indica (Rice)
Taxonomic identifier39946 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03129

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03129

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03129

Subcellular location

Plastidchloroplast HAMAP-Rule MF_03129.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Chloroplast Potential
Chain49 – 384336Lipoyl synthase 2, chloroplastic HAMAP-Rule MF_03129
PRO_0000398865

Sites

Metal binding1081Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1131Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1191Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1451Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1491Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1521Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
B8B016 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 9DA63FA586CDFFA1

FASTA38441,545
        10         20         30         40         50         60 
MAAYCSRVYH HHPVSPSTMQ GSLARPSIHA GSASLTFRAR PNSVSIVRCD ADSPPEGSAV 

        70         80         90        100        110        120 
AGWAPPGPYT GRDPAARKPA WLRQRAAQGE KYARLRESLG ELKLNTVCVE AQCPNIGECW 

       130        140        150        160        170        180 
NGGGGAGGDG DGIATATIML LGDTCTRGCR FCAVKTSNKP PPPDALEPLR TAVAVASWGV 

       190        200        210        220        230        240 
DYVVLTSVDR DDLPDGGSGH FAQTVKALKE LKPGILVECL TSDFRGDLEA VSSLASSGLD 

       250        260        270        280        290        300 
VFAHNIETVR SLQRIVRDPR AAYDQSLAVL KHAKNCKDGM VTKSSIMLGL GETDEEVKQT 

       310        320        330        340        350        360 
MCDLRAIDVD ILTLGQYLQP TERHLRVREY VTPEKFDFWK EYGESLGFLY VASGPLVRSS 

       370        380 
YRAGELFVQN LVRRKKAELA PTLQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000130 Genomic DNA. Translation: EEC79513.1.

3D structure databases

ProteinModelPortalB8B016.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os05g43576.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneB8B016.

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235998.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLISC2_ORYSI
AccessionPrimary (citable) accession number: B8B016
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways