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B8AA76 (LISC1_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase 1, chloroplastic

EC=2.8.1.8
Alternative name(s):
Lipoate synthase 1
Short name=LS 1
Short name=Lip-syn 1
Lipoate synthase, plastidial 1
Short name=LIP1p 1
Lipoic acid synthase 1
Gene names
Name:LIP1P-1
ORF Names:OsI_03888
OrganismOryza sativa subsp. indica (Rice)
Taxonomic identifier39946 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03129

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03129

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03129

Subcellular location

Plastidchloroplast Potential HAMAP-Rule MF_03129.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Sequence caution

The sequence EEC71548.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3737Chloroplast Potential
Chain38 – 370333Lipoyl synthase 1, chloroplastic HAMAP-Rule MF_03129
PRO_0000398863

Sites

Metal binding951Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1001Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1061Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1321Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1361Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1391Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
B8AA76 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 650FF00DCC0060AB

FASTA37039,940
        10         20         30         40         50         60 
MMQSSLARPL PRPPIRPACG NPVCRSRPGS VSVARCRAEA APPAPAPAAR RAAGPYTGRD 

        70         80         90        100        110        120 
PEVKKPAWLR QRAAQGEKYA RLRESIGELK LNTVCVEAQC PNIGECWNGG GGAGGEGDGI 

       130        140        150        160        170        180 
ATATIMVLGD TCTRGCRFCA VKTSNKPPPP DPLEPLNTAL AVASWGVDYV VLTSVDRDDL 

       190        200        210        220        230        240 
PDGGSSHFAQ TVRALKELKP GILVECLTSD FRGDLEAVSA LANSGLDVFA HNIETVRSLQ 

       250        260        270        280        290        300 
RIVRDPRAGY DQSLAVLKHA KSCKEGMITK SSIMLGLGET DEEVKQAMID LRAIGVDILT 

       310        320        330        340        350        360 
LGQYLQPTER HLTVREYVTP EKFQFWKEYG ESVGFRYVAS GPLVRSSYRA GELFVQNLVR 

       370 
NNKPKLPASS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000126 Genomic DNA. Translation: EEC71548.1. Sequence problems.

3D structure databases

ProteinModelPortalB8AA76.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneB8AA76.

Phylogenomic databases

eggNOGCOG0320.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLISC1_ORYSI
AccessionPrimary (citable) accession number: B8AA76
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 30 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways