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Protein

Lipoyl synthase 1, chloroplastic

Gene

LIP1P-1

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (OsI_15083), Lipoyl synthase 1, chloroplastic (LIP1P-1), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase 2, chloroplastic (LIP1P-2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi95Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi100Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi106Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi132Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi136Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi139Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-OSA-389661 Glyoxylate metabolism and glycine degradation
UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1, chloroplastic (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 1UniRule annotation
Short name:
LS 1UniRule annotation
Short name:
Lip-syn 1UniRule annotation
Lipoate synthase, plastidial 1UniRule annotation
Short name:
LIP1p 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Gene namesi
Name:LIP1P-1UniRule annotation
ORF Names:OsI_03888
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000007015 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37ChloroplastUniRule annotationAdd BLAST37
ChainiPRO_000039886338 – 370Lipoyl synthase 1, chloroplasticAdd BLAST333

Proteomic databases

PRIDEiB8AA76

Expressioni

Gene expression databases

ExpressionAtlasiB8AA76 differential

Interactioni

Protein-protein interaction databases

STRINGi39946.BGIOSGA004525-PA

Structurei

3D structure databases

ProteinModelPortaliB8AA76
SMRiB8AA76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
OrthoDBiEOG093604CP

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
MF_03129 Lipoyl_synth_plantC, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR027526 Lipoyl_synth_chlpt
IPR007197 rSAM
PANTHERiPTHR10949:SF14 PTHR10949:SF14, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8AA76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQSSLARPL PRPPIRPACG NPVCRSRPGS VSVARCRAEA APPAPAPAAR
60 70 80 90 100
RAAGPYTGRD PEVKKPAWLR QRAAQGEKYA RLRESIGELK LNTVCVEAQC
110 120 130 140 150
PNIGECWNGG GGAGGEGDGI ATATIMVLGD TCTRGCRFCA VKTSNKPPPP
160 170 180 190 200
DPLEPLNTAL AVASWGVDYV VLTSVDRDDL PDGGSSHFAQ TVRALKELKP
210 220 230 240 250
GILVECLTSD FRGDLEAVSA LANSGLDVFA HNIETVRSLQ RIVRDPRAGY
260 270 280 290 300
DQSLAVLKHA KSCKEGMITK SSIMLGLGET DEEVKQAMID LRAIGVDILT
310 320 330 340 350
LGQYLQPTER HLTVREYVTP EKFQFWKEYG ESVGFRYVAS GPLVRSSYRA
360 370
GELFVQNLVR NNKPKLPASS
Length:370
Mass (Da):39,940
Last modified:October 5, 2010 - v2
Checksum:i650FF00DCC0060AB
GO

Sequence cautioni

The sequence EEC71548 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000126 Genomic DNA Translation: EEC71548.1 Sequence problems.

Similar proteinsi

Entry informationi

Entry nameiLISC1_ORYSI
AccessioniPrimary (citable) accession number: B8AA76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: May 23, 2018
This is version 48 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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