ID   B8A5H6_DANRE            Unreviewed;       919 AA.
AC   B8A5H6; A0A8M1NG49;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   27-MAR-2024, entry version 99.
DE   RecName: Full=hexokinase {ECO:0000256|ARBA:ARBA00012324};
DE            EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
GN   Name=hkdc1 {ECO:0000313|Ensembl:ENSDARP00000056522,
GN   ECO:0000313|RefSeq:NP_001108597.1,
GN   ECO:0000313|ZFIN:ZDB-GENE-030131-9801};
GN   Synonyms=cb370 {ECO:0000313|RefSeq:NP_001108597.1}, sb:cb370
GN   {ECO:0000313|RefSeq:NP_001108597.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000056522};
RN   [1] {ECO:0000313|RefSeq:NP_001108597.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RX   PubMed=19087890;
RA   Gonzalez-Alvarez R., Ortega-Cuellar D., Hernandez-Mendoza A.,
RA   Moreno-Arriola E., Villasenor-Mendoza K., Galvez-Mariscal A.,
RA   Perez-Cruz M.E., Morales-Salas I., Velazquez-Arellano A.;
RT   "The hexokinase gene family in the zebrafish: structure, expression,
RT   functional and phylogenetic analysis.";
RL   Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 152:189-195(2009).
RN   [2] {ECO:0000313|Ensembl:ENSDARP00000056522, ECO:0000313|Proteomes:UP000000437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000056522};
RX   PubMed=23594743; DOI=10.1038/nature12111;
RG   Genome Reference Consortium Zebrafish;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA   Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA   Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA   Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA   Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA   Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA   Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA   Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA   Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA   Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA   Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA   Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA   Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA   Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA   Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA   Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA   Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA   Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA   Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA   Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA   Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA   Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA   Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3] {ECO:0000313|Ensembl:ENSDARP00000056522}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000056522};
RG   Ensembl;
RL   Submitted (AUG-2013) to UniProtKB.
RN   [4] {ECO:0000313|RefSeq:NP_001108597.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RX   PubMed=25759395;
RA   Dai Z., Wang H., Jin X., Wang H., He J., Liu M., Yin Z., Sun Y., Lou Q.;
RT   "Depletion of suppressor of cytokine signaling-1a causes hepatic steatosis
RT   and insulin resistance in zebrafish.";
RL   Am. J. Physiol. Endocrinol. Metab. 308:E849-E859(2015).
RN   [5] {ECO:0000313|RefSeq:NP_001108597.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RX   PubMed=27189481;
RA   Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA   Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA   Bobe J.;
RT   "Gene evolution and gene expression after whole genome duplication in fish:
RT   the PhyloFish database.";
RL   BMC Genomics 17:368-368(2016).
RN   [6] {ECO:0000313|RefSeq:NP_001108597.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RX   PubMed=27458428;
RA   Gao Y., Dai Z., Shi C., Zhai G., Jin X., He J., Lou Q., Yin Z.;
RT   "Depletion of Myostatin b Promotes Somatic Growth and Lipid Metabolism in
RT   Zebrafish.";
RL   Front. Endocrinol. 7:88-88(2016).
RN   [7] {ECO:0000313|RefSeq:NP_001108597.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RX   PubMed=28252024;
RA   Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA   Choudhary J.S., Emes R.D., Grant S.G.;
RT   "Evolution of complexity in the zebrafish synapse proteome.";
RL   Nat. Commun. 8:14613-14613(2017).
RN   [8] {ECO:0000313|RefSeq:NP_001108597.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RX   PubMed=29351486;
RA   Yang B.Y., Zhai G., Gong Y.L., Su J.Z., Peng X.Y., Shang G.H., Han D.,
RA   Jin J.Y., Liu H.K., Du Z.Y., Yin Z., Xie S.Q.;
RT   "Different physiological roles of insulin receptors in mediating nutrient
RT   metabolism in zebrafish.";
RL   Am. J. Physiol. Endocrinol. Metab. 315:E38-E51(2018).
RN   [9] {ECO:0000313|RefSeq:NP_001108597.1}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001108597.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 1/4.
CC       {ECO:0000256|ARBA:ARBA00004888}.
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC       {ECO:0000256|ARBA:ARBA00005028}.
CC   -!- SIMILARITY: Belongs to the hexokinase family.
CC       {ECO:0000256|ARBA:ARBA00009225}.
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DR   EMBL; BX546482; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001108597.1; NM_001115125.1.
DR   STRING; 7955.ENSDARP00000056522; -.
DR   PaxDb; 7955-ENSDARP00000056522; -.
DR   PeptideAtlas; B8A5H6; -.
DR   Ensembl; ENSDART00000056523; ENSDARP00000056522; ENSDARG00000038703.
DR   Ensembl; ENSDART00000056523.6; ENSDARP00000056522.5; ENSDARG00000038703.6.
DR   GeneID; 321224; -.
DR   KEGG; dre:321224; -.
DR   AGR; ZFIN:ZDB-GENE-030131-9801; -.
DR   CTD; 80201; -.
DR   ZFIN; ZDB-GENE-030131-9801; hkdc1.
DR   eggNOG; KOG1369; Eukaryota.
DR   HOGENOM; CLU_014393_1_0_1; -.
DR   OMA; PNEITRG; -.
DR   OrthoDB; 5481886at2759; -.
DR   PhylomeDB; B8A5H6; -.
DR   TreeFam; TF314238; -.
DR   UniPathway; UPA00109; UER00180.
DR   UniPathway; UPA00242; -.
DR   Proteomes; UP000000437; Alternate scaffold 13.
DR   Proteomes; UP000000437; Chromosome 13.
DR   Bgee; ENSDARG00000038703; Expressed in intestine and 13 other cell types or tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR   GO; GO:0004340; F:glucokinase activity; IBA:GO_Central.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:0046835; P:carbohydrate phosphorylation; IBA:GO_Central.
DR   GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR   GO; GO:0006006; P:glucose metabolic process; IBA:GO_Central.
DR   GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR   GO; GO:0001678; P:intracellular glucose homeostasis; IBA:GO_Central.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR   Gene3D; 3.30.420.40; -; 2.
DR   Gene3D; 3.40.367.20; -; 2.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR001312; Hexokinase.
DR   InterPro; IPR019807; Hexokinase_BS.
DR   InterPro; IPR022673; Hexokinase_C.
DR   InterPro; IPR022672; Hexokinase_N.
DR   PANTHER; PTHR19443; HEXOKINASE; 1.
DR   PANTHER; PTHR19443:SF28; HEXOKINASE HKDC1; 1.
DR   Pfam; PF00349; Hexokinase_1; 2.
DR   Pfam; PF03727; Hexokinase_2; 2.
DR   PRINTS; PR00475; HEXOKINASE.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR   PROSITE; PS00378; HEXOKINASE_1; 1.
DR   PROSITE; PS51748; HEXOKINASE_2; 2.
PE   1: Evidence at protein level;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:B8A5H6};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          22..219
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          226..459
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
FT   DOMAIN          470..667
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          673..907
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
SQ   SEQUENCE   919 AA;  102548 MW;  E06A9CAC14BF6FDB CRC64;
     MFAVHLLSFY FSKLQEDQIK KVDRFLYAMR LSEDQLKDIS ARFRLEMEKG LSSESNAAAA
     VKMLPTHVYS TPDGSEKGEF LALDLGGSKF KVLQVKVSED GKGKVEMESE TFPIPEEIVN
     GRGTELFEHV AESLKSFLQK HHINHTRKPL GFTFSFPCAQ SKIDEGVLLS WSKNFKVRGV
     QGTNVVQSLR KAIRKVGDLD VDVLAMVNDT VGAMMTCGYD DQNCEVGVIV GTGTNACYME
     EMRHIDLVEG DEGRMCINTE LGAFGDDGVL DDFITSFDRE IDAASINPGK QLFEKMVSGM
     YMGELVRLIL LRMAKKGLLF RGQISDALRT KGTFHTNHMC LIEQYKSGLE NTKEILEDLG
     LNPSDDDCIA VQHVCTIVSF RSANLVAAAL AAILTRIREN KKLKTLRTTV GVDGTVYRTH
     PQYPKRLHKV VRHLVPDCHV RFVLSESGSA KGAAMVTAVA QRLASQRKEI DDTLAAFALT
     STQLQEVKQK MHVELERGLK KETHPTASVK MLPTYVYRTP DGTERGKYLA LDLGGTNFRV
     LVVKIRTGMR NSVRMYNKIY AIPLEIMQGT GEELFDHIVQ CISDFLDYMG MKNTRLPLGF
     TFSFPCNQTG IDKGDLVCWT KGFKATDCEG YDVVDMLREA IKRRNEFDLD IVAIVNDTVG
     TMMTCAYEDP KCQIGLIAGT GSNVCYMEEM KNIEIVEGDE GQMCVNTEWG GFGENDNIED
     IRTRYDREVD SGSLNAGKQR FEKMTSGMYL GEIVRQILID LTKRGFLFRG RITERLKTKG
     IFETKFLSQI ESDRLALLQV RCILQSLGLD STCDDSIIVK EVCGAVSRRA AQLCGAGMAA
     IVDKIRENRG LDRLDITVGV DGTLYKLHPH FSTILKETVR ELAPNCKVDF ILSEDGSGKG
     AALITAVARQ HHVQKQEQN
//