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B8A031

- LIAS_MAIZE

UniProt

B8A031 - LIAS_MAIZE

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Protein
Lipoyl synthase, mitochondrial
Gene
LIP1
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi121 – 1211Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi145 – 1451Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi148 – 1481Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiB8A031.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 383Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398846
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Structurei

3D structure databases

ProteinModelPortaliB8A031.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.
OMAiEWLRRPI.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8A031-1 [UniParc]FASTAAdd to Basket

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MHGRRHLAAS LTRALIQAPS RSISSTPSLL QTLDPSVPSP PAAGAGRLAE    50
LRRRLQADAP SLGDFTYSVE VGTRQRPLPK PKWMKETVPG GAKYAAIKAK 100
LRELKLHTVC EEARCPNLGE CWSGGETGTA TATIMILGDT CTRGCRFCNV 150
KTSRTPPPPD PDEPSNVAQA IASWGLEYIV ITSVDRDDLP DQGSGHFAET 200
VQKLKALKPE MLIEALVPDF RGDPSCVEKV ATSGLHVFAH NIETVEELQR 250
NVRDYRANFK QSIDVLEMAK EYAPPGTLTK TSIMLGCGET PDQVIRTMEK 300
VRAADVDVIT FGQYMRPSKR HMPVSEYVTP EAFEKYRALG VEMGFRYVAS 350
GPMVRSSYKA GEFYIKAMIE AERAKGTAAD SSA 383
Length:383
Mass (Da):41,886
Last modified:March 3, 2009 - v1
Checksum:iD049717212C2F537
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT054923 mRNA. Translation: ACL53530.1.
BT061114 mRNA. Translation: ACN25811.1.
BT086520 mRNA. Translation: ACR36873.1.
RefSeqiNP_001146235.1. NM_001152763.1.
UniGeneiZm.6442.

Genome annotation databases

GeneIDi100279807.
KEGGizma:100279807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT054923 mRNA. Translation: ACL53530.1 .
BT061114 mRNA. Translation: ACN25811.1 .
BT086520 mRNA. Translation: ACR36873.1 .
RefSeqi NP_001146235.1. NM_001152763.1.
UniGenei Zm.6442.

3D structure databases

ProteinModelPortali B8A031.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100279807.
KEGGi zma:100279807.

Organism-specific databases

Gramenei B8A031.

Phylogenomic databases

HOGENOMi HOG000235998.
KOi K03644.
OMAi EWLRRPI.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: B73.

Entry informationi

Entry nameiLIAS_MAIZE
AccessioniPrimary (citable) accession number: B8A031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 3, 2009
Last modified: July 9, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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