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Protein

Outer envelope protein 61

Gene

OEP61

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in protein import into the endoplasmic reticulum (ER). May function as chaperone docking protein during post-translational protein translocation into the ER. Chaperone receptor mediating Hsp70-dependent protein targeting to chloroplasts. Interacts specifically with some chloroplast precursors, but not with mitochondrial precursors. Able to select precursors for delivery to the chloroplast translocase independently of Hsp70.2 Publications

GO - Biological processi

  • cytosol to ER transport Source: TAIR
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Outer envelope protein 61
Alternative name(s):
Tetratricopeptide repeat domain-containing protein 7
Gene namesi
Name:OEP61
Synonyms:TPR7
Ordered Locus Names:At5g21990
ORF Names:T6G21.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G21990
TAIRilocus:504956437 AT5G21990

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 525Cytoplasmic1 PublicationAdd BLAST525
Transmembranei526 – 546HelicalSequence analysisAdd BLAST21
Topological domaini547 – 554LumenalSequence analysis8

Keywords - Cellular componenti

Chloroplast, Endoplasmic reticulum, Membrane, Plastid, Plastid outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181K → E: Weak binding to HSP70-1 and HSP90-2. 1 Publication1
Mutagenesisi185R → A: Loss of binding to HSP70-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004140211 – 554Outer envelope protein 61Add BLAST554

Proteomic databases

PaxDbiB7ZWR6
PRIDEiB7ZWR6

PTM databases

iPTMnetiB7ZWR6

Expressioni

Tissue specificityi

Ubiquitous. Highest expression in leaves and lowest in roots.1 Publication

Gene expression databases

ExpressionAtlasiB7ZWR6 baseline and differential

Interactioni

Subunit structurei

Interacts (via TPR region) with HSP70-1, but not with HSP90-2. Interacts with ERDJ2A and ERDJ2B. In the ER membrane, associates with ERDJ2 in membrane complexes of 140 and 200 kDa and specifically interacts with the HSP70 and HSP90 chaperones via its TPR domain.2 Publications

Protein-protein interaction databases

BioGridi17534, 12 interactors
IntActiB7ZWR6, 1 interactor
MINTiB7ZWR6
STRINGi3702.AT5G21990.1

Structurei

3D structure databases

ProteinModelPortaliB7ZWR6
SMRiB7ZWR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 136TPR 1Add BLAST34
Repeati180 – 213TPR 2Add BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 50Met-richAdd BLAST50
Compositional biasi447 – 481Met-richAdd BLAST35

Domaini

The TPR region (103-213) is necessary for interaction with HSP70-1 while the linker domain (214-530) facilitates selective recognition of chloroplast precursor complexes.

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQSZ Eukaryota
ENOG410ZI5X LUCA
HOGENOMiHOG000240818
InParanoidiB7ZWR6
OMAiMANPELM
OrthoDBiEOG093606KF
PhylomeDBiB7ZWR6

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR023566 PPIase_FKBP
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PANTHERiPTHR43811 PTHR43811, 1 hit
PfamiView protein in Pfam
PF00515 TPR_1, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 3 hits
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 2 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

B7ZWR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNGLMDPEM IRLAQDQMSR MTPADFARIQ QQMMSNPDLM NMATESMKNM
60 70 80 90 100
RPEDLKQAAE QLKHTRPEDM AEISEKMAKA SPEDIAAMRA HADAQFTYQI
110 120 130 140 150
NAAQMLKKQG NELHSRGNFS DAAEKYLRAK NNLKEIPSSK GGAILLACSL
160 170 180 190 200
NLMSCYLKTN QHEECIKEGS EVLGYDARNV KALYRRGQAY RDLGLFEDAV
210 220 230 240 250
SDLSKAHEVS PEDETIADVL RDVKERLAVE GPGKASRGVV IEDITEENNV
260 270 280 290 300
TSGENKKPSK EANGHAQGVK TDVDGLQALR DNPEAIRTFQ NFISKTDPDT
310 320 330 340 350
LAALSGGKAG DMSPDMFKTA SSMIGKMSPE EIQKMVQTAS SFKGDNPFAP
360 370 380 390 400
TAPSTENGFT PTPDMLKLAS DMMGKMSPEE RERMFNMASS LKANAPASTS
410 420 430 440 450
YGNAEASEPR ESLGASGSSS GNSFVAPRSG FEPSIPSAPP ADLQEQMRNQ
460 470 480 490 500
MKDPAMRQMF TSMIKNMNPE MMASMSEQFG MKLSQEDAAK AQQAMASLSP
510 520 530 540 550
DALEKMMRWA DRAQTGMEKA KKAKKWLFGK GGLIFAILML VLAMVLHRLG

YIGN
Length:554
Mass (Da):60,757
Last modified:March 3, 2009 - v1
Checksum:iA72209F5DF86C3E4
GO

Sequence cautioni

The sequence CAC34492 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248N → K in BAC43348 (PubMed:11910074).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL589883 Genomic DNA Translation: CAC34492.1 Different initiation.
CP002688 Genomic DNA Translation: AED92964.1
AK118755 mRNA Translation: BAC43348.1
BT053758 mRNA Translation: ACL13985.1
RefSeqiNP_680187.2, NM_147882.3
UniGeneiAt.20053

Genome annotation databases

EnsemblPlantsiAT5G21990.1; AT5G21990.1; AT5G21990
GeneIDi832259
GrameneiAT5G21990.1; AT5G21990.1; AT5G21990
KEGGiath:AT5G21990

Similar proteinsi

Entry informationi

Entry nameiOEP61_ARATH
AccessioniPrimary (citable) accession number: B7ZWR6
Secondary accession number(s): Q8GWM6, Q9C588
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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