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B7ZQA9

- P5F12_XENLA

UniProt

B7ZQA9 - P5F12_XENLA

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Protein

POU domain, class 5, transcription factor 1.2

Gene

pou5f1.2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi312 – 37160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. gastrulation Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.2
Alternative name(s):
POU class V protein oct-911 Publication
Short name:
XlPOU91
Short name:
Xoct-91Imported
Gene namesi
Name:pou5f1.2
Synonyms:oct-91Imported
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252587. pou5f3.1.

Subcellular locationi

Nucleus By similarityPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445POU domain, class 5, transcription factor 1.2PRO_0000390497Add
BLAST

Expressioni

Tissue specificityi

Initially (stage 9) expressed in all regions of the embryo, becoming localized to the ventroposterior regions by early neurula stages. In adults, expressed at a low level in the brain.2 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed at low levels in oocytes, with expression increasing rapidly during gastrulation and peaking during late gastrulation before declining through neurulation.2 Publications

Interactioni

Subunit structurei

Interacts with the transcription factors tcf7l1/tcf3 and vegt.1 Publication

Protein-protein interaction databases

IntActiB7ZQA9. 2 interactions.
MINTiMINT-4979404.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini218 – 29275POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi167 – 21650Ser-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053782.
KOiK09367.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7ZQA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNQQTYPSF THNPALMPDG SGQYNLGTYT GMARHPHQAQ AFFPFSGVKS
60 70 80 90 100
DYGDLGGQTT SVGDTSAWNP LTSLDSANQL GISGQGNPFK NLKREREDDE
110 120 130 140 150
EKSESPEPKC SPPSLPPAYY THAWNPTTTF WSQVSSSGTT VVSKPLPTPL
160 170 180 190 200
QPGDKCDPVE ANKIFTSSPD KSGESGISSL DNSRCSSATS SSSGGTNVGT
210 220 230 240 250
PRSLSRGASD GLSSDSEEEA PNSGEMEQFA KDLKHKRITM GYTQADVGYA
260 270 280 290 300
LGVLFGKTFS QTTICRFESL QLSFKNMCKL KPLLRSWLHE VENNENLQEI
310 320 330 340 350
ISRGQIIPQV QKRKHRTSIE NNVKCTLENY FMQCSKPSAQ EIAQIARELN
360 370 380 390 400
MEKDVVRVWF CNRRQKGKRQ VYPYIRENGG EPYDAPQTLT PPSQGPFPLP
410 420 430 440
QVMPSQVFPT VPLGANPTIY VPTYHKNDMF PQAMHHGIGM GNQGN
Length:445
Mass (Da):49,036
Last modified:March 3, 2009 - v1
Checksum:iC981C1F3A2EDA93C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti248 – 2481G → A(PubMed:1358592)Curated
Sequence conflicti295 – 2951E → K in AAA49999. (PubMed:1732736)Curated
Sequence conflicti333 – 3331Q → G(PubMed:1358592)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60077 mRNA. Translation: AAA49999.1.
BC169741 mRNA. Translation: AAI69741.1.
BC169743 mRNA. Translation: AAI69743.1.
PIRiC42022.
RefSeqiNP_001081342.1. NM_001087873.1.
UniGeneiXl.870.
Xl.871.

Genome annotation databases

GeneIDi397784.
KEGGixla:397784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60077 mRNA. Translation: AAA49999.1 .
BC169741 mRNA. Translation: AAI69741.1 .
BC169743 mRNA. Translation: AAI69743.1 .
PIRi C42022.
RefSeqi NP_001081342.1. NM_001087873.1.
UniGenei Xl.870.
Xl.871.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi B7ZQA9. 2 interactions.
MINTi MINT-4979404.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397784.
KEGGi xla:397784.

Organism-specific databases

CTDi 397784.
Xenbasei XB-GENE-6252587. pou5f3.1.

Phylogenomic databases

HOVERGENi HBG053782.
KOi K09367.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequential expression of multiple POU proteins during amphibian early development."
    Hinkley C.S., Martin J.F., Leibham D., Perry M.
    Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Gastrula1 Publication.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: GastrulaImported.
  3. "Localized expression of a Xenopus POU gene depends on cell-autonomous transcriptional activation and induction-dependent inactivation."
    Frank D., Harland R.M.
    Development 115:439-448(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 236-362, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Gastrula1 Publication.
  4. "Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
    Cao Y., Siegel D., Knochel W.
    Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
    Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
    EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TCF7L1 AND VEGT.

Entry informationi

Entry nameiP5F12_XENLA
AccessioniPrimary (citable) accession number: B7ZQA9
Secondary accession number(s): Q03917, Q9PSI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3