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Protein

POU domain, class 5, transcription factor 1.2

Gene

pou5f1.2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi312 – 371HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • gastrulation Source: UniProtKB

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.2
Alternative name(s):
POU class V protein oct-911 Publication
Short name:
XlPOU91
Short name:
Xoct-91Imported
Gene namesi
Name:pou5f1.2
Synonyms:oct-91Imported
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252587. pou5f3.1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904971 – 445POU domain, class 5, transcription factor 1.2Add BLAST445

Expressioni

Tissue specificityi

Initially (stage 9) expressed in all regions of the embryo, becoming localized to the ventroposterior regions by early neurula stages. In adults, expressed at a low level in the brain.2 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed at low levels in oocytes, with expression increasing rapidly during gastrulation and peaking during late gastrulation before declining through neurulation.2 Publications

Interactioni

Subunit structurei

Interacts with the transcription factors tcf7l1/tcf3 and vegt.1 Publication

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

IntActiB7ZQA9. 2 interactors.
MINTiMINT-4979404.

Structurei

3D structure databases

SMRiB7ZQA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini218 – 292POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi167 – 216Ser-richSequence analysisAdd BLAST50

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence analysis

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053782.
KOiK09367.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR015585. POU_dom_5.
PANTHERiPTHR11636:SF100. PTHR11636:SF100. 1 hit.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.

Sequencei

Sequence statusi: Complete.

B7ZQA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNQQTYPSF THNPALMPDG SGQYNLGTYT GMARHPHQAQ AFFPFSGVKS
60 70 80 90 100
DYGDLGGQTT SVGDTSAWNP LTSLDSANQL GISGQGNPFK NLKREREDDE
110 120 130 140 150
EKSESPEPKC SPPSLPPAYY THAWNPTTTF WSQVSSSGTT VVSKPLPTPL
160 170 180 190 200
QPGDKCDPVE ANKIFTSSPD KSGESGISSL DNSRCSSATS SSSGGTNVGT
210 220 230 240 250
PRSLSRGASD GLSSDSEEEA PNSGEMEQFA KDLKHKRITM GYTQADVGYA
260 270 280 290 300
LGVLFGKTFS QTTICRFESL QLSFKNMCKL KPLLRSWLHE VENNENLQEI
310 320 330 340 350
ISRGQIIPQV QKRKHRTSIE NNVKCTLENY FMQCSKPSAQ EIAQIARELN
360 370 380 390 400
MEKDVVRVWF CNRRQKGKRQ VYPYIRENGG EPYDAPQTLT PPSQGPFPLP
410 420 430 440
QVMPSQVFPT VPLGANPTIY VPTYHKNDMF PQAMHHGIGM GNQGN
Length:445
Mass (Da):49,036
Last modified:March 3, 2009 - v1
Checksum:iC981C1F3A2EDA93C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248G → A (PubMed:1358592).Curated1
Sequence conflicti295E → K in AAA49999 (PubMed:1732736).Curated1
Sequence conflicti333Q → G (PubMed:1358592).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60077 mRNA. Translation: AAA49999.1.
BC169741 mRNA. Translation: AAI69741.1.
BC169743 mRNA. Translation: AAI69743.1.
PIRiC42022.
RefSeqiNP_001081342.1. NM_001087873.1.
UniGeneiXl.871.

Genome annotation databases

GeneIDi397784.
KEGGixla:397784.

Similar proteinsi

Entry informationi

Entry nameiP5F12_XENLA
AccessioniPrimary (citable) accession number: B7ZQA9
Secondary accession number(s): Q03917, Q9PSI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 3, 2009
Last modified: September 27, 2017
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families