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B7ZQA9

- P5F12_XENLA

UniProt

B7ZQA9 - P5F12_XENLA

Protein

POU domain, class 5, transcription factor 1.2

Gene

pou5f1.2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi312 – 37160HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: UniProtKB
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. gastrulation Source: UniProtKB

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1.2
    Alternative name(s):
    POU class V protein oct-911 Publication
    Short name:
    XlPOU91
    Short name:
    Xoct-91Imported
    Gene namesi
    Name:pou5f1.2
    Synonyms:oct-91Imported
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6252587. pou5f3.1.

    Subcellular locationi

    Nucleus By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445POU domain, class 5, transcription factor 1.2PRO_0000390497Add
    BLAST

    Expressioni

    Tissue specificityi

    Initially (stage 9) expressed in all regions of the embryo, becoming localized to the ventroposterior regions by early neurula stages. In adults, expressed at a low level in the brain.2 Publications

    Developmental stagei

    Expressed both maternally and zygotically. Expressed at low levels in oocytes, with expression increasing rapidly during gastrulation and peaking during late gastrulation before declining through neurulation.2 Publications

    Interactioni

    Subunit structurei

    Interacts with the transcription factors tcf7l1/tcf3 and vegt.1 Publication

    Protein-protein interaction databases

    IntActiB7ZQA9. 2 interactions.
    MINTiMINT-4979404.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini218 – 29275POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi167 – 21650Ser-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    HOVERGENiHBG053782.
    KOiK09367.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7ZQA9-1 [UniParc]FASTAAdd to Basket

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    MYNQQTYPSF THNPALMPDG SGQYNLGTYT GMARHPHQAQ AFFPFSGVKS    50
    DYGDLGGQTT SVGDTSAWNP LTSLDSANQL GISGQGNPFK NLKREREDDE 100
    EKSESPEPKC SPPSLPPAYY THAWNPTTTF WSQVSSSGTT VVSKPLPTPL 150
    QPGDKCDPVE ANKIFTSSPD KSGESGISSL DNSRCSSATS SSSGGTNVGT 200
    PRSLSRGASD GLSSDSEEEA PNSGEMEQFA KDLKHKRITM GYTQADVGYA 250
    LGVLFGKTFS QTTICRFESL QLSFKNMCKL KPLLRSWLHE VENNENLQEI 300
    ISRGQIIPQV QKRKHRTSIE NNVKCTLENY FMQCSKPSAQ EIAQIARELN 350
    MEKDVVRVWF CNRRQKGKRQ VYPYIRENGG EPYDAPQTLT PPSQGPFPLP 400
    QVMPSQVFPT VPLGANPTIY VPTYHKNDMF PQAMHHGIGM GNQGN 445
    Length:445
    Mass (Da):49,036
    Last modified:March 3, 2009 - v1
    Checksum:iC981C1F3A2EDA93C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti248 – 2481G → A(PubMed:1358592)Curated
    Sequence conflicti295 – 2951E → K in AAA49999. (PubMed:1732736)Curated
    Sequence conflicti333 – 3331Q → G(PubMed:1358592)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60077 mRNA. Translation: AAA49999.1.
    BC169741 mRNA. Translation: AAI69741.1.
    BC169743 mRNA. Translation: AAI69743.1.
    PIRiC42022.
    RefSeqiNP_001081342.1. NM_001087873.1.
    UniGeneiXl.870.
    Xl.871.

    Genome annotation databases

    GeneIDi397784.
    KEGGixla:397784.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60077 mRNA. Translation: AAA49999.1 .
    BC169741 mRNA. Translation: AAI69741.1 .
    BC169743 mRNA. Translation: AAI69743.1 .
    PIRi C42022.
    RefSeqi NP_001081342.1. NM_001087873.1.
    UniGenei Xl.870.
    Xl.871.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi B7ZQA9. 2 interactions.
    MINTi MINT-4979404.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397784.
    KEGGi xla:397784.

    Organism-specific databases

    CTDi 397784.
    Xenbasei XB-GENE-6252587. pou5f3.1.

    Phylogenomic databases

    HOVERGENi HBG053782.
    KOi K09367.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequential expression of multiple POU proteins during amphibian early development."
      Hinkley C.S., Martin J.F., Leibham D., Perry M.
      Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Gastrula1 Publication.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: GastrulaImported.
    3. "Localized expression of a Xenopus POU gene depends on cell-autonomous transcriptional activation and induction-dependent inactivation."
      Frank D., Harland R.M.
      Development 115:439-448(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 236-362, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Gastrula1 Publication.
    4. "Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
      Cao Y., Siegel D., Knochel W.
      Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
      Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
      EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TCF7L1 AND VEGT.

    Entry informationi

    Entry nameiP5F12_XENLA
    AccessioniPrimary (citable) accession number: B7ZQA9
    Secondary accession number(s): Q03917, Q9PSI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3