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B7ZQA9 (P5F12_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1.2
Alternative name(s):
POU class V protein oct-91
Short name=XlPOU91
Short name=Xoct-91
Gene names
Name:pou5f1.2
Synonyms:oct-91
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation. Ref.1 Ref.4

Subunit structure

Interacts with the transcription factors tcf7l1/tcf3 and vegt. Ref.5

Subcellular location

Nucleus By similarity UniProtKB Q01860.

Tissue specificity

Initially (stage 9) expressed in all regions of the embryo, becoming localized to the ventroposterior regions by early neurula stages. In adults, expressed at a low level in the brain. Ref.1 Ref.3

Developmental stage

Expressed both maternally and zygotically. Expressed at low levels in oocytes, with expression increasing rapidly during gastrulation and peaking during late gastrulation before declining through neurulation. Ref.1 Ref.3

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445POU domain, class 5, transcription factor 1.2
PRO_0000390497

Regions

Domain218 – 29275POU-specific
DNA binding312 – 37160Homeobox
Compositional bias167 – 21650Ser-rich

Experimental info

Sequence conflict2481G → A Ref.3
Sequence conflict2951E → K in AAA49999. Ref.1
Sequence conflict3331Q → G Ref.3

Sequences

Sequence LengthMass (Da)Tools
B7ZQA9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: C981C1F3A2EDA93C

FASTA44549,036
        10         20         30         40         50         60 
MYNQQTYPSF THNPALMPDG SGQYNLGTYT GMARHPHQAQ AFFPFSGVKS DYGDLGGQTT 

        70         80         90        100        110        120 
SVGDTSAWNP LTSLDSANQL GISGQGNPFK NLKREREDDE EKSESPEPKC SPPSLPPAYY 

       130        140        150        160        170        180 
THAWNPTTTF WSQVSSSGTT VVSKPLPTPL QPGDKCDPVE ANKIFTSSPD KSGESGISSL 

       190        200        210        220        230        240 
DNSRCSSATS SSSGGTNVGT PRSLSRGASD GLSSDSEEEA PNSGEMEQFA KDLKHKRITM 

       250        260        270        280        290        300 
GYTQADVGYA LGVLFGKTFS QTTICRFESL QLSFKNMCKL KPLLRSWLHE VENNENLQEI 

       310        320        330        340        350        360 
ISRGQIIPQV QKRKHRTSIE NNVKCTLENY FMQCSKPSAQ EIAQIARELN MEKDVVRVWF 

       370        380        390        400        410        420 
CNRRQKGKRQ VYPYIRENGG EPYDAPQTLT PPSQGPFPLP QVMPSQVFPT VPLGANPTIY 

       430        440 
VPTYHKNDMF PQAMHHGIGM GNQGN 

« Hide

References

« Hide 'large scale' references
[1]"Sequential expression of multiple POU proteins during amphibian early development."
Hinkley C.S., Martin J.F., Leibham D., Perry M.
Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Gastrula.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Gastrula.
[3]"Localized expression of a Xenopus POU gene depends on cell-autonomous transcriptional activation and induction-dependent inactivation."
Frank D., Harland R.M.
Development 115:439-448(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 236-362, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Gastrula.
[4]"Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
Cao Y., Siegel D., Knochel W.
Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TCF7L1 AND VEGT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60077 mRNA. Translation: AAA49999.1.
BC169741 mRNA. Translation: AAI69741.1.
BC169743 mRNA. Translation: AAI69743.1.
PIRC42022.
RefSeqNP_001081342.1. NM_001087873.1.
UniGeneXl.870.
Xl.871.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActB7ZQA9. 2 interactions.
MINTMINT-4979404.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397784.
KEGGxla:397784.

Organism-specific databases

CTD397784.

Phylogenomic databases

HOVERGENHBG053782.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5F12_XENLA
AccessionPrimary (citable) accession number: B7ZQA9
Secondary accession number(s): Q03917, Q9PSI7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families