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Protein

Kinesin-like protein KIF7

Gene

Kif7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for hedgehog signaling regulation: acts as both a negative and positive regulator of sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO, through both SUFU-dependent and -independent mechanisms. Involved in the regulation of microtubular dynamics. Required for proper organization of the ciliary tip and control of ciliary localization of SUFU-GLI2 complexes. Required for localization of GLI3 to cilia in response to Shh. Negatively regulates Shh signaling by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates Shh signaling by preventing the processing of the transcription factor GLI3 into its repressor form. In keratinocytes, promotes the dissociation of SUFU-GLI2 complexes, GLI2 nuclear translocation and Shh signaling activation. Involved in the regulation of epidermal differentiation and chondrocyte development.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi94 – 1018ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • aorta development Source: MGI
  • cardiac septum development Source: MGI
  • coronary vasculature development Source: MGI
  • microtubule-based movement Source: GO_Central
  • negative regulation of smoothened signaling pathway Source: UniProtKB
  • positive regulation of smoothened signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Repressor

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF7
Gene namesi
Name:Kif7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1098239. Kif7.

Subcellular locationi

GO - Cellular componenti

  • cilium Source: UniProtKB
  • kinesin complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

Pathology & Biotechi

Disruption phenotypei

Mutations appear to be the cause of the matariki (maki) phenotype, characterized by expanded motor neuron domain in the embryonic day 10.5 (E10.5) neural tube. Motor neurons are increased in number and expand dorsally. Mutants die at the end of gestation, when other phenotypes characteristic of elevated Shh signaling are apparent, including preaxial polydactyly. Knockout mice have defects in developmental patterning and die at birth with severe malformations, including exencephaly and polydactyly.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi130 – 1301L → P in maki; expanded motor neuron domain in the E10.5 neural tube. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13481348Kinesin-like protein KIF7PRO_0000405978Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei903 – 9031PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiB7ZNG0.
PaxDbiB7ZNG0.
PRIDEiB7ZNG0.

PTM databases

iPTMnetiB7ZNG0.
PhosphoSiteiB7ZNG0.

Expressioni

Tissue specificityi

Expressed in heart, lung, liver, kidney, testis, spleen and cerebellum.1 Publication

Interactioni

Subunit structurei

Can form homodimers and interacts with microtubules. Interacts with GLI1 and SMO (By similarity). Interacts with GLI2, GLI3 and SUFU. Interacts with NPHP1 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-61761N.
STRINGi10090.ENSMUSP00000061806.

Structurei

3D structure databases

ProteinModelPortaliB7ZNG0.
SMRiB7ZNG0. Positions 12-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 349335Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili480 – 54263Sequence analysisAdd
BLAST
Coiled coili698 – 1057360Sequence analysisAdd
BLAST
Coiled coili1109 – 1211103Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi624 – 6318Poly-Glu

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
HOGENOMiHOG000068072.
HOVERGENiHBG054858.
InParanoidiB7ZNG0.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 5 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B7ZNG0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLEAQRLPG AEEAPVRVAL RVRPLLPKEL LHGHQSCLRV EPERGRITLG
60 70 80 90 100
RDRHFGFHVV LGEDTGQEAV YQACVQPLLE AFFEGFNATV FAYGQTGSGK
110 120 130 140 150
TYTMGEASVA SLHEDEQGII PRAMAEAFKL IDENDLLDCL VHVSYLELYK
160 170 180 190 200
EEFRDLLEVG TASRDIQLRE DDRGNVVLCG VKEVDVEGLD EVLSLLEMGN
210 220 230 240 250
AARHTGATHF NRLSSRSHTV FTVTLEQRGR TPSRLPRPAA GHLLVSKFHF
260 270 280 290 300
VDLAGSERVL KTGSTGERLK ESIQINSTLL ALGNVISALG DPQRRGSHIP
310 320 330 340 350
YRDSKITRIL KDSLGGNAKT VMIACVSPSS SDFDETLNTL NYASRAQNIR
360 370 380 390 400
NRATVNWHPE AERVPEEQAA GARGPPRHRS ETRIIHRGRR VPCPAVGSAA
410 420 430 440 450
VAAGLGAECA RCRARTSAAY SLLRELQAEP GLPGAAARKV RDWLCAVEGE
460 470 480 490 500
RSTLSSASGP DSGIESAPAE DQAAQGTSGR KGDEGTQQLL TLQSQVARLE
510 520 530 540 550
EENRDFLAAL EDAMEQYKLQ SDRLREQQEE MVELRLRLEL AQPGWGAPGL
560 570 580 590 600
LQGLPPGSFV PRPHTAPLGG AHTHMLGMMP STCLPGEEVS SEQQVVSGKE
610 620 630 640 650
VKAEVLAQAD KLRSASSTTS EEEGEEEEEE EEEEEEPPRR TLYLRRNGIS
660 670 680 690 700
NWSQRAGLSP GSPPDRKGPE VCPEEPAAAI PAPQAVGSGK VPVQTRQAPA
710 720 730 740 750
AMASEWRLAQ AQQKIRELAI NIRMKEELIG ELVRTGKAAQ ALNRQHSQRI
760 770 780 790 800
RELEQEAERV RAELCEGQRQ LRELEGREPQ DASERSRLQE FRKRVAAAQS
810 820 830 840 850
QVQVLKEKKQ ATERLVSLSA QSETRLQELE RNVQLMRRQQ GQLQRRLREE
860 870 880 890 900
TEQKRRLETE MNKRQHRVKE LELKHEQQQK ILKIKTEEIA AFQRKRRSGS
910 920 930 940 950
NGSVVSLEQQ QKIEEQKKWL DQEMEKVLQQ RRALEELGEE LRKREVILAK
960 970 980 990 1000
KEALMQEKTG LESKRLRSSQ ALNEDIVRVS SRLEHLEKEL SEKSGQLRQG
1010 1020 1030 1040 1050
SAQNQQQIRG EIDTLRQEKD SLLKQRLEID SKLRQGSLLS PEEERTLFQL
1060 1070 1080 1090 1100
DEAIEALDAA IEYKNEAITC RQRVLRASAS LLSQCEMNLM AKLSYLSSSE
1110 1120 1130 1140 1150
TRALLCKYFD KVVTLREEQH QQQIAFSELE MQLEEQQRLV YWLEVALERQ
1160 1170 1180 1190 1200
RLEMDRQLTL QQKEHEQNVQ LLLQQGRDHL GEGLADSKRQ YEARIHALEK
1210 1220 1230 1240 1250
ELGRHMWINQ ELKQKLSAGS TAGQSQGCER RSLCLENRQC LGNEDGLHPA
1260 1270 1280 1290 1300
APEPLWQSSL LEGVSRVWDE SRDLVHAPLP LTWKRSSLCS EQGSSEESRV
1310 1320 1330 1340
RETTEPPVGR VLPMGEVGLS WNFGPLPKPR WEPRRTSPGM IDVRKNPL
Length:1,348
Mass (Da):151,624
Last modified:March 3, 2009 - v1
Checksum:i76D4B7387965C17C
GO
Isoform 2 (identifier: B7ZNG0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     912-912: Missing.

Show »
Length:1,347
Mass (Da):151,496
Checksum:iB1CE4178EC01961D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1226 – 12261Q → R in AAI41046 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei912 – 9121Missing in isoform 2. 1 PublicationVSP_040736

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC141045 mRNA. Translation: AAI41046.1.
BC145222 mRNA. Translation: AAI45223.1.
CCDSiCCDS52278.1. [B7ZNG0-2]
CCDS80739.1. [B7ZNG0-1]
UniGeneiMm.101633.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC141045 mRNA. Translation: AAI41046.1.
BC145222 mRNA. Translation: AAI45223.1.
CCDSiCCDS52278.1. [B7ZNG0-2]
CCDS80739.1. [B7ZNG0-1]
UniGeneiMm.101633.

3D structure databases

ProteinModelPortaliB7ZNG0.
SMRiB7ZNG0. Positions 12-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61761N.
STRINGi10090.ENSMUSP00000061806.

PTM databases

iPTMnetiB7ZNG0.
PhosphoSiteiB7ZNG0.

Proteomic databases

MaxQBiB7ZNG0.
PaxDbiB7ZNG0.
PRIDEiB7ZNG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1098239. Kif7.

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
HOGENOMiHOG000068072.
HOVERGENiHBG054858.
InParanoidiB7ZNG0.

Miscellaneous databases

PROiB7ZNG0.
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 5 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF7_MOUSE
AccessioniPrimary (citable) accession number: B7ZNG0
Secondary accession number(s): B2RUB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 3, 2009
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.