B7ZMP1 (XPP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase 3
Short name=X-Pro aminopeptidase 3
EC=3.4.11.9

Alternative name(s):
Aminopeptidase P3
Short name=APP3

Gene names

Name:

Xpnpep3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Mitochondrion Ref.4.
Tissue specificity	Expressed in brain, kidney, heart, liver, skeletal muscle and testis. Ref.4
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro glomerular filtration Inferred from electronic annotation. Source: Compara protein processing Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrion Inferred from direct assay PubMed 14651853PubMed 18614015Ref.4. Source: MGI
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B7ZMP1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B7ZMP1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-386: FTAPQAELYEAVLEIQRACLTLCSPGTSLENIYS → LLENTALIMLAITSGWMSMTLQTCLGHSLCSLEW
     387-506: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Probable Xaa-Pro aminopeptidase 3		PRO_0000401208
Sites
	Metal binding	331	1	Manganese 2 By similarity
	Metal binding	342	1	Manganese 1 By similarity
	Metal binding	342	1	Manganese 2 By similarity
	Metal binding	423	1	Manganese 1 By similarity
	Metal binding	450	1	Manganese 1 By similarity
	Metal binding	474	1	Manganese 1 By similarity
	Metal binding	474	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	353 – 386	34	FTAPQ…ENIYS → LLENTALIMLAITSGWMSMT LQTCLGHSLCSLEW in isoform 2.		VSP_040145
	Alternative sequence	387 – 506	120	Missing in isoform 2.		VSP_040146

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: E1F36BDA1F92C023	FASTA	506	56,677
	10 20 30 40 50 60 MPSLLSTPKL APVLARLRGL SGCMSCLQRR YSLQPAPVKK IPNRYLGQPS PVTHPHLLRP 70 80 90 100 110 120 GEVTPGLSQV EYALRRHKLM ALVHKEAQGH SGTDHTVVVL SNPTYYMSND IPYTFHQDNN 130 140 150 160 170 180 FLYLCGFQEP DSILVLQSFS GKQLPSHKAM LFVPRRDPGR ELWDGPRSGT DGAIALTGVD 190 200 210 220 230 240 EAYPLEEFQH LLPKLRAETN MVWYDWMKPS HAQLHSDYMQ PLTEAKARSK NKVRSVQQLI 250 260 270 280 290 300 QRLRLVKSPS EIKRMQIAGK LTSEAFIETM FASKAPIDEA FLYAKFEFEC RARGADILAY 310 320 330 340 350 360 PPVVAGGNRS NTLHYVKNNQ LIKDGEMVLL DGGCESSCYV SDITRTWPVN GRFTAPQAEL 370 380 390 400 410 420 YEAVLEIQRA CLTLCSPGTS LENIYSMMLT LIGQKLKDLG ITKTSKESAF KAARKYCPHH 430 440 450 460 470 480 VGHYLGMDVH DTPDMPRSLP LQPGMVITVE PGIYIPEDDR DAPEKFRGLG VRIEDDVVVT 490 500 QDSPLILSAD CPKEMNDIEQ ICSRTS
	Isoform 2 [UniParc]. Checksum: CD47003E01441698	FASTA	386	43,367
	10 20 30 40 50 60 MPSLLSTPKL APVLARLRGL SGCMSCLQRR YSLQPAPVKK IPNRYLGQPS PVTHPHLLRP 70 80 90 100 110 120 GEVTPGLSQV EYALRRHKLM ALVHKEAQGH SGTDHTVVVL SNPTYYMSND IPYTFHQDNN 130 140 150 160 170 180 FLYLCGFQEP DSILVLQSFS GKQLPSHKAM LFVPRRDPGR ELWDGPRSGT DGAIALTGVD 190 200 210 220 230 240 EAYPLEEFQH LLPKLRAETN MVWYDWMKPS HAQLHSDYMQ PLTEAKARSK NKVRSVQQLI 250 260 270 280 290 300 QRLRLVKSPS EIKRMQIAGK LTSEAFIETM FASKAPIDEA FLYAKFEFEC RARGADILAY 310 320 330 340 350 360 PPVVAGGNRS NTLHYVKNNQ LIKDGEMVLL DGGCESSCYV SDITRTWPVN GRLLENTALI 370 380 MLAITSGWMS MTLQTCLGHS LCSLEW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: NOD. Tissue: Thymus.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy." O'Toole J.F., Liu Y., Davis E.E., Westlake C.J., Attanasio M., Otto E.A., Seelow D., Nurnberg G., Becker C., Nuutinen M., Karppa M., Ignatius J., Uusimaa J., Pakanen S., Jaakkola E., van den Heuvel L.P., Fehrenbach H., Wiggins R. , Goyal M., Zhou W., Wolf M.T., Wise E., Helou J., Allen S.J., Murga-Zamalloa C.A., Ashraf S., Chaki M., Heeringa S., Chernin G., Hoskins B.E., Chaib H., Gleeson J., Kusakabe T., Suzuki T., Isaac R.E., Quarmby L.M., Tennant B., Fujioka H., Tuominen H., Hassinen I., Lohi H., van Houten J.L., Rotig A., Sayer J.A., Rolinski B., Freisinger P., Madhavan S.M., Herzer M., Madignier F., Prokisch H., Nurnberg P., Jackson P.K., Khanna H., Katsanis N., Hildebrandt F. J. Clin. Invest. 120:791-802(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	Erratum O'Toole J.F., Liu Y., Davis E.E., Westlake C.J., Attanasio M., Otto E.A., Seelow D., Nurnberg G., Becker C., Nuutinen M., Karppa M., Ignatius J., Uusimaa J., Pakanen S., Jaakkola E., van den Heuvel L.P., Fehrenbach H., Wiggins R. , Goyal M., Zhou W., Wolf M.T., Wise E., Helou J., Allen S.J., Murga-Zamalloa C.A., Ashraf S., Chaki M., Heeringa S., Chernin G., Hoskins B.E., Chaib H., Gleeson J., Kusakabe T., Suzuki T., Isaac R.E., Quarmby L.M., Tennant B., Fujioka H., Tuominen H., Hassinen I., Lohi H., van Houten J.L., Rotig A., Sayer J.A., Rolinski B., Freisinger P., Madhavan S.M., Herzer M., Madignier F., Prokisch H., Nurnberg P., Jackson P.K., Khanna H., Katsanis N., Hildebrandt F. J. Clin. Invest. 120:1362-1362(2010)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK088324 mRNA. Translation: BAC40282.1. CH466550 Genomic DNA. Translation: EDL04573.1. CH466550 Genomic DNA. Translation: EDL04574.1. BC137569 mRNA. Translation: AAI37570.1. BC144717 mRNA. Translation: AAI44718.1.
IPI	IPI00221972. IPI00923106.
RefSeq	NP_796284.1. NM_177310.2.
UniGene	Mm.390833.
3D structure databases
ProteinModelPortal	B7ZMP1.
SMR	B7ZMP1. Positions 72-499.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4110631.
STRING	10090.ENSMUSP00000038331.
Protein family/group databases
MEROPS	M24.026.
Proteomic databases
PaxDb	B7ZMP1.
PRIDE	B7ZMP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041609; ENSMUSP00000038331; ENSMUSG00000022401. ENSMUST00000163754; ENSMUSP00000132822; ENSMUSG00000022401.
GeneID	321003.
KEGG	mmu:321003.
UCSC	uc007wwo.1. mouse. uc011zwj.1. mouse.
Organism-specific databases
CTD	63929.
MGI	MGI:2445217. Xpnpep3.
Phylogenomic databases
eggNOG	COG0006.
GeneTree	ENSGT00550000074909.
HOGENOM	HOG000008762.
HOVERGEN	HBG057305.
InParanoid	Q8BHT9.
KO	K01262.
OMA	KEVKHIE.
OrthoDB	EOG4KPT9J.
Gene expression databases
ArrayExpress	B7ZMP1.
Bgee	B7ZMP1.
Genevestigator	B7ZMP1.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
ProtoNet	Search...
Other
NextBio	397845.
SOURCE	Search...

Entry information

Entry name

XPP3_MOUSE

Accession

Primary (citable) accession number: B7ZMP1
Secondary accession number(s): Q8BHT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	March 3, 2009
Last modified:	May 29, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program