Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B7ZAQ6 (GPHRA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi pH regulator A
Alternative name(s):
Protein GPR89A
Putative MAPK-activating protein PM01
Putative NF-kappa-B-activating protein 90
Gene names
Name:GPR89A
Synonyms:GPHRA, GPR89, SH120
ORF Names:CGI-13, UNQ192/PRO218
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance By similarity. Ref.3 Ref.9

Subunit structure

Homotrimer By similarity. Ref.9

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein By similarity Ref.9.

Tissue specificity

Ubiquitous. Ref.9

Miscellaneous

Does not seem to be able to bind GTP.

Sequence similarities

Belongs to the Golgi pH regulator (TC 1.A.38) family. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B7ZAQ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B7ZAQ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.
Isoform 3 (identifier: B7ZAQ6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 455455Golgi pH regulator A
PRO_0000223260

Regions

Transmembrane5 – 2521Helical; Potential
Transmembrane46 – 6621Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane114 – 13421Helical; Potential
Transmembrane150 – 17021Helical; Potential
Transmembrane290 – 31021Helical; Potential
Transmembrane343 – 36321Helical; Potential
Transmembrane378 – 39821Helical; Potential
Transmembrane425 – 44521Helical; Potential

Amino acid modifications

Glycosylation1801N-linked (GlcNAc...) Potential
Glycosylation2431N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 120120Missing in isoform 2.
VSP_017247
Alternative sequence1 – 2525Missing in isoform 3.
VSP_039346

Experimental info

Sequence conflict1001S → G in BAG63359. Ref.5
Sequence conflict2431N → MPD in BAD96943. Ref.6
Sequence conflict3791I → T in BAD96943. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: D831F66E682643F9

FASTA45552,917
        10         20         30         40         50         60 
MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEIRQYVV QVIFSVTFAF SCTMFELIIF 

        70         80         90        100        110        120 
EILGVLNSSS RYFHWKMNLC VILLILVFMV PFYIGYFIVS NIRLLHKQRL LFSCLLWLTF 

       130        140        150        160        170        180 
MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN 

       190        200        210        220        230        240 
VTDTDILALE RRLLQTMDMI ISKKKRMAMA RRTMFQKGEV HNKPSGFWGM IKSVTTSASG 

       250        260        270        280        290        300 
SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK 

       310        320        330        340        350        360 
IFMATINIVF DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL 

       370        380        390        400        410        420 
ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPLEYRTII TEVLGELQFN 

       430        440        450 
FYHRWFDVIF LVSALSSILF LYLAHKQAPE KQMAP 

« Hide

Isoform 2 [UniParc].

Checksum: AC5F09C2DA129937
Show »

FASTA33538,484
Isoform 3 [UniParc].

Checksum: 60065DCCB3B1A31B
Show »

FASTA43049,986

References

« Hide 'large scale' references
[1]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), FUNCTION.
Tissue: Lung fibroblast.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Embryo and Testis.
[6]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Gastric mucosa.
[7]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain, Lung and Testis.
[9]"GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus."
Maeda Y., Ide T., Koike M., Uchiyama Y., Kinoshita T.
Nat. Cell Biol. 10:1135-1145(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
[10]"Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis."
Pandey S., Nelson D.C., Assmann S.M.
Cell 136:136-148(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF GTP-BINDING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF132947 mRNA. Translation: AAD27722.1.
AY358966 mRNA. Translation: AAQ89325.1.
AB097024 mRNA. Translation: BAC77377.1.
AB097025 mRNA. Translation: BAC77378.1.
BT006679 mRNA. Translation: AAP35325.1.
AK021758 mRNA. Translation: BAG51045.1.
AK301939 mRNA. Translation: BAG63359.1.
AK316371 mRNA. Translation: BAH14742.1.
AK223223 mRNA. Translation: BAD96943.1.
AL390725, BX511042, CR628408 Genomic DNA. Translation: CAI13712.2.
BX511042, AL390725, CR628408 Genomic DNA. Translation: CAI13224.2.
CR628408, AL390725, BX511042 Genomic DNA. Translation: CAM28366.1.
BC003187 mRNA. Translation: AAH03187.1.
BC067816 mRNA. Translation: AAH67816.1.
BC146880 mRNA. Translation: AAI46881.1.
CCDSCCDS41377.1. [B7ZAQ6-1]
CCDS44207.2. [B7ZAQ6-3]
RefSeqNP_001091081.1. NM_001097612.1. [B7ZAQ6-1]
NP_001091082.2. NM_001097613.2. [B7ZAQ6-3]
NP_057418.1. NM_016334.3. [B7ZAQ6-1]
XP_005277458.1. XM_005277401.2. [B7ZAQ6-1]
XP_005277459.1. XM_005277402.2. [B7ZAQ6-3]
XP_005277461.1. XM_005277404.2. [B7ZAQ6-2]
XP_006711440.1. XM_006711377.1. [B7ZAQ6-3]
XP_006711441.1. XM_006711378.1. [B7ZAQ6-2]
XP_006711555.1. XM_006711492.1. [B7ZAQ6-1]
XP_006711556.1. XM_006711493.1. [B7ZAQ6-2]
UniGeneHs.504320.
Hs.559115.
Hs.645432.

3D structure databases

ProteinModelPortalB7ZAQ6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119554. 1 interaction.
575849. 1 interaction.
MINTMINT-4723292.
STRING9606.ENSP00000319673.

PTM databases

PhosphoSiteB7ZAQ6.

Proteomic databases

MaxQBB7ZAQ6.
PaxDbB7ZAQ6.
PRIDEB7ZAQ6.

Protocols and materials databases

DNASU51463.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000313835; ENSP00000319673; ENSG00000117262. [B7ZAQ6-1]
ENST00000454423; ENSP00000410203; ENSG00000117262. [B7ZAQ6-2]
ENST00000462900; ENSP00000432248; ENSG00000117262. [B7ZAQ6-3]
ENST00000534502; ENSP00000434495; ENSG00000117262. [B7ZAQ6-3]
ENST00000577475; ENSP00000462369; ENSG00000264337. [B7ZAQ6-3]
ENST00000582339; ENSP00000461974; ENSG00000264337. [B7ZAQ6-1]
ENST00000582624; ENSP00000463679; ENSG00000264337. [B7ZAQ6-2]
ENST00000583232; ENSP00000463147; ENSG00000264337. [B7ZAQ6-3]
GeneID51463.
653519.
KEGGhsa:51463.
hsa:653519.
UCSCuc001eos.2. human. [B7ZAQ6-1]

Organism-specific databases

CTD51463.
653519.
GeneCardsGC01M145764.
HGNCHGNC:31984. GPR89A.
MIM612821. gene.
neXtProtNX_B7ZAQ6.
PharmGKBPA134986137.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG295277.
HOVERGENHBG054909.
PhylomeDBB7ZAQ6.
TreeFamTF313484.

Gene expression databases

BgeeB7ZAQ6.

Family and domain databases

InterProIPR025969. ABA_GPCR_dom.
IPR022535. Golgi_pH-regulator_cons_dom.
IPR015672. GPHR.
[Graphical view]
PANTHERPTHR15948. PTHR15948. 1 hit.
PfamPF12430. ABA_GPCR. 1 hit.
PF12537. DUF3735. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGPR89A. human.
GeneWikiGPR89B.
NextBio55087.
PROB7ZAQ6.
SOURCESearch...

Entry information

Entry nameGPHRA_HUMAN
AccessionPrimary (citable) accession number: B7ZAQ6
Secondary accession number(s): A6NN37 expand/collapse secondary AC list , B2RUV3, B3KMN3, B4DXE7, Q53FQ9, Q5T2V8, Q5T5P5, Q659E2, Q6NVY5, Q9P0S4, Q9Y302
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: July 9, 2014
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM