B7ZAQ6 (GPHRA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi pH regulator A

Alternative name(s):
Protein GPR89A
Putative MAPK-activating protein PM01
Putative NF-kappa-B-activating protein 90

Gene names

Name:	GPR89A
Synonyms:	GPHRA, GPR89, SH120
ORF Names:	CGI-13, UNQ192/PRO218

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance By similarity. Ref.3 Ref.9
Subunit structure	Homotrimer By similarity. Ref.9
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein By similarity Ref.9.
Tissue specificity	Ubiquitous. Ref.9
Miscellaneous	Does not seem to be able to bind GTP.
Sequence similarities	Belongs to the Golgi pH regulator (TC 1.A.38) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Protein transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular pH reduction Inferred from direct assay Ref.9. Source: UniProtKB positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from mutant phenotype Ref.3. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi cisterna membrane Inferred from direct assay Ref.9. Source: UniProtKB Golgi-associated vesicle membrane Inferred from direct assay Ref.9. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	signal transducer activity Inferred from mutant phenotype Ref.3. Source: UniProtKB voltage-gated anion channel activity Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B7ZAQ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B7ZAQ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.

Isoform 3 (identifier: B7ZAQ6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Golgi pH regulator A		PRO_0000223260
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	290 – 310	21	Helical; Potential
	Transmembrane	343 – 363	21	Helical; Potential
	Transmembrane	378 – 398	21	Helical; Potential
	Transmembrane	425 – 445	21	Helical; Potential
Amino acid modifications
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	243	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 120	120	Missing in isoform 2.		VSP_017247
	Alternative sequence	1 – 25	25	Missing in isoform 3.		VSP_039346
Experimental info
	Sequence conflict	100	1	S → G in BAG63359. Ref.5
	Sequence conflict	243	1	N → MPD in BAD96943. Ref.6
	Sequence conflict	379	1	I → T in BAD96943. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: D831F66E682643F9	FASTA	455	52,917
	10 20 30 40 50 60 MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEIRQYVV QVIFSVTFAF SCTMFELIIF 70 80 90 100 110 120 EILGVLNSSS RYFHWKMNLC VILLILVFMV PFYIGYFIVS NIRLLHKQRL LFSCLLWLTF 130 140 150 160 170 180 MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN 190 200 210 220 230 240 VTDTDILALE RRLLQTMDMI ISKKKRMAMA RRTMFQKGEV HNKPSGFWGM IKSVTTSASG 250 260 270 280 290 300 SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK 310 320 330 340 350 360 IFMATINIVF DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL 370 380 390 400 410 420 ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPLEYRTII TEVLGELQFN 430 440 450 FYHRWFDVIF LVSALSSILF LYLAHKQAPE KQMAP
	Isoform 2 [UniParc]. Checksum: AC5F09C2DA129937	FASTA	335	38,484
	10 20 30 40 50 60 MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN 70 80 90 100 110 120 VTDTDILALE RRLLQTMDMI ISKKKRMAMA RRTMFQKGEV HNKPSGFWGM IKSVTTSASG 130 140 150 160 170 180 SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK 190 200 210 220 230 240 IFMATINIVF DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL 250 260 270 280 290 300 ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPLEYRTII TEVLGELQFN 310 320 330 FYHRWFDVIF LVSALSSILF LYLAHKQAPE KQMAP
	Isoform 3 [UniParc]. Checksum: 60065DCCB3B1A31B	FASTA	430	49,986
	10 20 30 40 50 60 MRQLFKDYEI RQYVVQVIFS VTFAFSCTMF ELIIFEILGV LNSSSRYFHW KMNLCVILLI 70 80 90 100 110 120 LVFMVPFYIG YFIVSNIRLL HKQRLLFSCL LWLTFMYFFW KLGDPFPILS PKHGILSIEQ 130 140 150 160 170 180 LISRVGVIGV TLMALLSGFG AVNCPYTYMS YFLRNVTDTD ILALERRLLQ TMDMIISKKK 190 200 210 220 230 240 RMAMARRTMF QKGEVHNKPS GFWGMIKSVT TSASGSENLT LIQQEVDALE ELSRQLFLET 250 260 270 280 290 300 ADLYATKERI EYSKTFKGKY FNFLGYFFSI YCVWKIFMAT INIVFDRVGK TDPVTRGIEI 310 320 330 340 350 360 TVNYLGIQFD VKFWSQHISF ILVGIIIVTS IRGLLITLTK FFYAISSSKS SNVIVLLLAQ 370 380 390 400 410 420 IMGMYFVSSV LLIRMSMPLE YRTIITEVLG ELQFNFYHRW FDVIFLVSAL SSILFLYLAH 430 KQAPEKQMAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), FUNCTION. Tissue: Lung fibroblast.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Embryo and Testis.
[6]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Gastric mucosa.
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Lung and Testis.
[9]	"GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." Maeda Y., Ide T., Koike M., Uchiyama Y., Kinoshita T. Nat. Cell Biol. 10:1135-1145(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
[10]	"Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis." Pandey S., Nelson D.C., Assmann S.M. Cell 136:136-148(2009) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF GTP-BINDING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF132947 mRNA. Translation: AAD27722.1. AY358966 mRNA. Translation: AAQ89325.1. AB097024 mRNA. Translation: BAC77377.1. AB097025 mRNA. Translation: BAC77378.1. BT006679 mRNA. Translation: AAP35325.1. AK021758 mRNA. Translation: BAG51045.1. AK301939 mRNA. Translation: BAG63359.1. AK316371 mRNA. Translation: BAH14742.1. AK223223 mRNA. Translation: BAD96943.1. AL390725, BX511042, CR628408 Genomic DNA. Translation: CAI13712.2. BX511042, AL390725, CR628408 Genomic DNA. Translation: CAI13224.2. CR628408, AL390725, BX511042 Genomic DNA. Translation: CAM28366.1. BC003187 mRNA. Translation: AAH03187.1. BC067816 mRNA. Translation: AAH67816.1. BC146880 mRNA. Translation: AAI46881.1.
IPI	IPI00472858. IPI00654550. IPI00967342.
RefSeq	NP_001091081.1. NM_001097612.1. NP_001091082.2. NM_001097613.2. NP_057418.1. NM_016334.3.
UniGene	Hs.504320. Hs.559115. Hs.645432.
3D structure databases
ProteinModelPortal	B7ZAQ6.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000319673.
PTM databases
PhosphoSite	B7ZAQ6.
Proteomic databases
PaxDb	B7ZAQ6.
PRIDE	B7ZAQ6.
Protocols and materials databases
DNASU	51463.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000313835; ENSP00000319673; ENSG00000117262. ENST00000454423; ENSP00000410203; ENSG00000117262. ENST00000462900; ENSP00000432248; ENSG00000117262. ENST00000534502; ENSP00000434495; ENSG00000117262. ENST00000577475; ENSP00000462369; ENSG00000264337. ENST00000582339; ENSP00000461974; ENSG00000264337. ENST00000582624; ENSP00000463679; ENSG00000264337. ENST00000583232; ENSP00000463147; ENSG00000264337.
GeneID	51463. 653519.
KEGG	hsa:51463. hsa:653519.
UCSC	uc001eos.2. human.
Organism-specific databases
CTD	51463. 653519.
GeneCards	GC01M145764.
HGNC	HGNC:31984. GPR89A.
MIM	612821. gene.
neXtProt	NX_B7ZAQ6.
PharmGKB	PA134986137.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG295277.
HOVERGEN	HBG054909.
OrthoDB	EOG476K05.
PhylomeDB	B7ZAQ6.
Gene expression databases
ArrayExpress	B7ZAQ6.
Bgee	B7ZAQ6.
GermOnline	ENSG00000117262. Homo sapiens.
Family and domain databases
InterPro	IPR025969. ABA_GPCR_dom. IPR022535. Golgi_pH-regulator_cons_dom. IPR015672. GPCR89-rel. [Graphical view]
PANTHER	PTHR15948. PTHR15948. 1 hit.
Pfam	PF12430. ABA_GPCR. 1 hit. PF12537. DUF3735. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GPR89A. human.
NextBio	55087.
SOURCE	Search...

Entry information

Entry name

GPHRA_HUMAN

Accession

Primary (citable) accession number: B7ZAQ6
Secondary accession number(s): A6NN37 , B2RUV3, B3KMN3, B4DXE7, Q53FQ9, Q5T2V8, Q5T5P5, Q659E2, Q6NVY5, Q9P0S4, Q9Y302

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	April 3, 2013
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.