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B7XK16 (SYIC_ENTBH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
ORF Names:EBI_22873
OrganismEnterocytozoon bieneusi (strain H348) (Microsporidian parasite) [Complete proteome]
Taxonomic identifier481877 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaEnterocytozoonidaeEnterocytozoon

Protein attributes

Sequence length953 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 953953Probable isoleucine--tRNA ligase, cytoplasmic
PRO_0000388379

Regions

Motif45 – 5511"HIGH" region By similarity
Motif634 – 6385"KMSKS" region By similarity

Sites

Binding site6371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7XK16 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 821531819140DFB2

FASTA953112,077
        10         20         30         40         50         60 
MSDKIIDFSK IEKDILKFWT ENKIFELCNK LSQGREEYVF FDGPPFATGL PHYGHILSGT 

        70         80         90        100        110        120 
IKDIIGRFYY QKGFHVERRF GWDCHGLPVE YEIDKKFNIT DRNQILKMGI DVYNHECRKI 

       130        140        150        160        170        180 
VQKYTNEWEE TVIRMGRWVD FKGSYKTMDL EFMESIWFIF KELFKRNKIY RGFKIMPFST 

       190        200        210        220        230        240 
ACKTALSNFE ASQNYKNVSD PSVLVTFKII NPKDVLHNYE NNDIEIFFVA WTTTPWTLPA 

       250        260        270        280        290        300 
NCALVINPDY EYAIFYINNN SKKLYIMLKN RIEVYFKQFC ITTVILGKEL IGVSYATLFN 

       310        320        330        340        350        360 
YYENYITRGF FKTIGGNFVT ITDGSGIVHT APAFGEEDYN IFLKLGLLKP DEIPPCHIDE 

       370        380        390        400        410        420 
NGKFTIAMEK YVNVPINNDI SLNGVYFKDA DKIILQILKP HLIYNSRIVH SYPFCWRSDT 

       430        440        450        460        470        480 
PLLYKLIPNW FVKITDIREE LLTQNKKINW IPETIKYKRF QNWLSNAKDW AISRNRFWGT 

       490        500        510        520        530        540 
PLPIWARYEN EIYNYTDLIC VGSIKELEDL TNTKITDLHR EHIDQLLIEH NGKTYKRIED 

       550        560        570        580        590        600 
VLDCWFESGS MPYAQNHLKG IKVNNSLIDK LDNINLNNAY LIKENFPADF IGEGLDQTRG 

       610        620        630        640        650        660 
WFYTLHVIST ILFNSPAFKN VIVNGIVLAE DGKKMSKRLK NYPDPMNIFN KYGADALRMY 

       670        680        690        700        710        720 
LISSPVVEAE NLRFNENGVK EILKTLLIPW YNTLVFYKEC QIQNHNHTLL LDAWIESELN 

       730        740        750        760        770        780 
ILINKVNIDM SEYKLNNILN YALDFIENLN NWYIRINRKE LKNNGLYLKT IIKRFSIVMS 

       790        800        810        820        830        840 
AFVPFFSDYS YQIVCSDKIS VHLEMIPEYK SINSDFNFIK CIIDGIRHLR EKYKLKLKKT 

       850        860        870        880        890        900 
LKEVIIVLDN ININKNLFEK YEAVIKSECN TLDIILNDIN DYSISTIIKP NFKELNKNKN 

       910        920        930        940        950 
VIKEKLDIIS KLTVNDIENI KNGTHDININ ELLIETYIKD IEYSGVFITL ELF 

« Hide

References

[1]"Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi."
Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J.
PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H348.
[2]"Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi."
Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.
PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H348.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ABGB01000039 Genomic DNA. Translation: EED43719.1.
RefSeqXP_002650344.1. XM_002650298.1.

3D structure databases

ProteinModelPortalB7XK16.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEB7XK16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8641969.

Organism-specific databases

EuPathDBMicrosporidiaDB:EBI_22873.

Phylogenomic databases

OrthoDBEOG7FNCGS.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYIC_ENTBH
AccessionPrimary (citable) accession number: B7XK16
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries