B7XK16 (SYIC_ENTBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable isoleucine--tRNA ligase, cytoplasmic
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

ORF Names:

EBI_22873

Organism

Enterocytozoon bieneusi (strain H348) (Microsporidian parasite) [Complete proteome]

Taxonomic identifier

481877 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Enterocytozoonidae › Enterocytozoon › Enterocytozoon bieneusi

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Probable isoleucine--tRNA ligase, cytoplasmic		PRO_0000388379
Regions
	Motif	45 – 55	11	"HIGH" region By similarity
	Motif	634 – 638	5	"KMSKS" region By similarity
Sites
	Binding site	637	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7XK16 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 821531819140DFB2	FASTA	953	112,077
	10 20 30 40 50 60 MSDKIIDFSK IEKDILKFWT ENKIFELCNK LSQGREEYVF FDGPPFATGL PHYGHILSGT 70 80 90 100 110 120 IKDIIGRFYY QKGFHVERRF GWDCHGLPVE YEIDKKFNIT DRNQILKMGI DVYNHECRKI 130 140 150 160 170 180 VQKYTNEWEE TVIRMGRWVD FKGSYKTMDL EFMESIWFIF KELFKRNKIY RGFKIMPFST 190 200 210 220 230 240 ACKTALSNFE ASQNYKNVSD PSVLVTFKII NPKDVLHNYE NNDIEIFFVA WTTTPWTLPA 250 260 270 280 290 300 NCALVINPDY EYAIFYINNN SKKLYIMLKN RIEVYFKQFC ITTVILGKEL IGVSYATLFN 310 320 330 340 350 360 YYENYITRGF FKTIGGNFVT ITDGSGIVHT APAFGEEDYN IFLKLGLLKP DEIPPCHIDE 370 380 390 400 410 420 NGKFTIAMEK YVNVPINNDI SLNGVYFKDA DKIILQILKP HLIYNSRIVH SYPFCWRSDT 430 440 450 460 470 480 PLLYKLIPNW FVKITDIREE LLTQNKKINW IPETIKYKRF QNWLSNAKDW AISRNRFWGT 490 500 510 520 530 540 PLPIWARYEN EIYNYTDLIC VGSIKELEDL TNTKITDLHR EHIDQLLIEH NGKTYKRIED 550 560 570 580 590 600 VLDCWFESGS MPYAQNHLKG IKVNNSLIDK LDNINLNNAY LIKENFPADF IGEGLDQTRG 610 620 630 640 650 660 WFYTLHVIST ILFNSPAFKN VIVNGIVLAE DGKKMSKRLK NYPDPMNIFN KYGADALRMY 670 680 690 700 710 720 LISSPVVEAE NLRFNENGVK EILKTLLIPW YNTLVFYKEC QIQNHNHTLL LDAWIESELN 730 740 750 760 770 780 ILINKVNIDM SEYKLNNILN YALDFIENLN NWYIRINRKE LKNNGLYLKT IIKRFSIVMS 790 800 810 820 830 840 AFVPFFSDYS YQIVCSDKIS VHLEMIPEYK SINSDFNFIK CIIDGIRHLR EKYKLKLKKT 850 860 870 880 890 900 LKEVIIVLDN ININKNLFEK YEAVIKSECN TLDIILNDIN DYSISTIIKP NFKELNKNKN 910 920 930 940 950 VIKEKLDIIS KLTVNDIENI KNGTHDININ ELLIETYIKD IEYSGVFITL ELF

References

[1]	"Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi." Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J. PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H348.
[2]	"Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi." Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S. PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABGB01000039 Genomic DNA. Translation: EED43719.1.
RefSeq	XP_002650344.1. XM_002650298.1.
3D structure databases
ProteinModelPortal	B7XK16.
ModBase	Search...
Proteomic databases
PRIDE	B7XK16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8641969.
Organism-specific databases
EuPathDB	MicrosporidiaDB:EBI_22873.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIC_ENTBH

Accession

Primary (citable) accession number: B7XK16

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	March 3, 2009
Last modified:	March 6, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program