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B7XHP6 (SYLC_ENTBH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable leucine--tRNA ligase, cytoplasmic

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
ORF Names:EBI_21702
OrganismEnterocytozoon bieneusi (strain H348) (Microsporidian parasite) [Complete proteome]
Taxonomic identifier481877 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaEnterocytozoonidaeEnterocytozoon

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Probable leucine--tRNA ligase, cytoplasmic
PRO_0000388414

Regions

Motif41 – 5111"HIGH" region By similarity
Motif552 – 5565"KMSKS" region By similarity

Sites

Binding site5551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7XHP6 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 2362357DA934B50A

FASTA860100,606
        10         20         30         40         50         60 
MSENNTKLKY LCDLEEKGRR QCMESEILVD KKKFFITFPY PYMNGKLHLG HLFSISKADI 

        70         80         90        100        110        120 
FSYYKEMQGF NVLFPFGFHC TGMPISASAK KLKMELANEG EIDISVVNIL KSLGFKLYCS 

       130        140        150        160        170        180 
GKCTNVECIC QFTDPFHWCR TFPKYCKASL VKYDANIDWR RSFITTDVNP YYDSFVKYQF 

       190        200        210        220        230        240 
TKLKNCNYIS FGKRYSIFCP VDNQICLDHD RRKGEGVKPI PIVLAILGNN ILVPVQEKDL 

       250        260        270        280        290        300 
NTIIDNNVEI VTQKEIQWIQ FSINNDVYVA EEDIYNNIKY QVENVYKIKT VTLDGLYPYI 

       310        320        330        340        350        360 
IYVNSNIRKS KINNKNKILT ENIYNKLIAD RHEKMELIKY DKFIVCYVPE DTVISRSGGK 

       370        380        390        400        410        420 
CTVALMDQWF LDYSNPEWKE KTKICLNKLT ASKDTKEKLE LALNWINKWG FSRNFGLGTK 

       430        440        450        460        470        480 
FPYDNSVLID SLSDSTIYMA FYTVKHLLFK DLEGKEPLFP IKYMGYAFWE YVFGNSNEIN 

       490        500        510        520        530        540 
FIDPNDLNAK KIVEDARSQF NYFYPIDLRV SGKDLINNHL IFFLMNHVAI FKENNWPKRI 

       550        560        570        580        590        600 
YTNGHLLLNG LKMSKSEGNF LSVDTALERF GTSATRMCLA ICGDTNEDAN FVESMANSFV 

       610        620        630        640        650        660 
LKLYTLSQNI FLISSNNQSV NVADYTLIDY YLISSIKINE EKTFTSYENI VYRDVIKYGF 

       670        680        690        700        710        720 
YNNLDTIETY EMFGGSNSNL KHWAYYNMMK QLYPVIPSLA RYFLNEVLTD ISIEFKNIIN 

       730        740        750        760        770        780 
QQYISGIEYI KELIHKINKF TKHDAKKVNL TLIKHYPPWK ELCMKKIDEL KLIHKNNNDL 

       790        800        810        820        830        840 
KKIVLKECSQ FIGENNRKKG ILFCMDYLLY KEKYIINFDE ELYTNTFKSI IEQETKKKII 

       850        860 
ILIKDDSTKG EPLCPEINSC 

« Hide

References

[1]"Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi."
Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J.
PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H348.
[2]"Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi."
Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.
PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H348.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ABGB01000015 Genomic DNA. Translation: EED44543.1.
RefSeqXP_002649524.1. XM_002649478.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8641125.

Organism-specific databases

EuPathDBMicrosporidiaDB:EBI_21702.

Phylogenomic databases

OrthoDBEOG7TTQH2.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYLC_ENTBH
AccessionPrimary (citable) accession number: B7XHP6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: March 3, 2009
Last modified: November 13, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries