ID   FTHS_VIBA3              Reviewed;         582 AA.
AC   B7VSJ2;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   27-MAR-2024, entry version 67.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=VS_II0809;
OS   Vibrio atlanticus (strain LGP32) (Vibrio splendidus (strain Mel32)).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=575788;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LGP32;
RA   Mazel D., Le Roux F.;
RT   "Vibrio splendidus str. LGP32 complete genome.";
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; FM954973; CAV26568.1; -; Genomic_DNA.
DR   AlphaFoldDB; B7VSJ2; -.
DR   SMR; B7VSJ2; -.
DR   STRING; 575788.VS_II0809; -.
DR   KEGG; vsp:VS_II0809; -.
DR   PATRIC; fig|575788.5.peg.764; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000009100; Chromosome 2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..582
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000185271"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   582 AA;  62374 MW;  742337700BC834BA CRC64;
     MLSDIDICRS TPLKNISEVA KQAGLHHNEH QPLGQYKSKV SLTSLERLAS QQDGKLVVVT
     AITPTPLGEG KTVTTIGLAQ GLAKINQSAM ACIRQPSMGP VFGVKGGAAG GGYSQVAPME
     QLNLHLTGDI HAVTVAHNLA SAAIDARLYH EQREGLEAFE ARSGLKALDI DPRRIVWRRV
     LDHNDRALRM ITVGKNEADK TINGFEREDG FDISAASELM AILALTDDLQ DLRKRIGRVV
     LAYNNQGLPL TADDFNVAGA MTVTMKDSIE PTLMQTLEGV PTLIHAGPFA NIAHGNSSII
     ADKIALKLSD FVVTEGGFGS DMGFEKACNI KVKASNKKPD CAVIVATLRG LKANSGLYDL
     RPGTPLPDSI FNDDQDALIA GFENLKWHIN NVKQYQVPTV VAINRFPQDS AQELNALKQM
     ITDFDPSVSV EVSEAFGQGG EGATQLAHAV VKACQKQSEF KPLYHSEQSL EEKLMAVAEV
     GYGAASISLS PLAKKQLAEF KLHGYSDLSV CLAKTPLSIS TEAHIKGAPS QFDVPVRELK
     LCAGAGFIYA LCGNVMTMPG LPDKPAFMSL DIDSKGNIVG LS
//