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B7VRL0 (NAPA_VIBSL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:VS_II0598
OrganismVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]
Taxonomic identifier575788 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 829800Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186379

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B7VRL0 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 0CA48A08E83D13C5

FASTA82992,614
        10         20         30         40         50         60 
MKMTRRAFVK ANAAASAAAV AGVTLPATAT NLIASSDQTK ITWDKAPCRF CGTGCSVLVG 

        70         80         90        100        110        120 
TQNGKVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGKDRLE QPLLRMKDGE FHKDGDFAPV 

       130        140        150        160        170        180 
SWDKAFDVMA EKWKAALKKN GPTGVGMFGS GQWTVMEGYA AVKLMKAGFR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVGAFM RTFGIDEPMG CYDDFEHADA FVLWGSNMAE MHPVLWTRIT DRRLSHPHVK 

       250        260        270        280        290        300 
VNVLSTYYHR SFELADTGYI FEPQSDLAIA NFIANYIIQN DAVNWEFVNK HTNFKQATTD 

       310        320        330        340        350        360 
IGYGLRDNDP LQKAAANPNS GKMTDISFED YKASVAEYTV EKASEMSGVS QDDLIKLAKQ 

       370        380        390        400        410        420 
YADPNIKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIS TPGNSPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFSHRLP ADMVVANPKH RKIAEDIWKL PEGTIPPKPG KHAVAQDRAL KDGTINAYWV 

       490        500        510        520        530        540 
MCNNNMQAGP NINTERLPGY RNPDNFIVCS DPYPTATAQA SDLILPTAMW IEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQAWYQQ VKTVGEAKSD LWQIMEFSKR FTVEEVWGEE LLAKAPEYRG KTMYDVLYKN 

       610        620        630        640        650        660 
GNVDAFPLSE AQELNDDAQA QGFYVQKGLF EEYAAFGRDH GHDLAPYDTY HKVRGLRWPV 

       670        680        690        700        710        720 
VDGKETLWRF KEGSDPYAKK GSGWDFYGKP DGKALIINAP YEAPPEVPSD EYDMWLCTGR 

       730        740        750        760        770        780 
VLEHWHTGTM TRRVPELYKA VPDALCYIHP ADAKARGLRR GDEVLIENKR GEVRVRVETR 

       790        800        810        820 
GRNRPPQGLV FVPFFDARIL INKLILDATD PLSKQTDYKK CPVKITKVS 

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References

[1]"Vibrio splendidus str. LGP32 complete genome."
Mazel D., Le Roux F.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LGP32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM954973 Genomic DNA. Translation: CAV26209.1.
RefSeqYP_002395192.1. NC_011744.2.

3D structure databases

ProteinModelPortalB7VRL0.
SMRB7VRL0. Positions 41-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING575788.VS_II0598.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAV26209; CAV26209; VS_II0598.
GeneID7137902.
KEGGvsp:VS_II0598.
PATRIC20149040. VBIVibSpl48387_0579.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAEYRGKTM.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycVSPL575788:GH64-3680-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_VIBSL
AccessionPrimary (citable) accession number: B7VRL0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families