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B7VRL0

- NAPA_VIBSL

UniProt

B7VRL0 - NAPA_VIBSL

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Protein
Periplasmic nitrate reductase
Gene
napA, VS_II0598
Organism
Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciVSPL575788:GH64-3680-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:VS_II0598
OrganismiVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
Taxonomic identifieri575788 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000009100: Chromosome 2

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal Reviewed prediction
Add
BLAST
Chaini30 – 829800Periplasmic nitrate reductaseUniRule annotation
PRO_1000186379Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi575788.VS_II0598.

Structurei

3D structure databases

ProteinModelPortaliB7VRL0.
SMRiB7VRL0. Positions 41-827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiEYRGKTM.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B7VRL0-1 [UniParc]FASTAAdd to Basket

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MKMTRRAFVK ANAAASAAAV AGVTLPATAT NLIASSDQTK ITWDKAPCRF    50
CGTGCSVLVG TQNGKVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGKDRLE 100
QPLLRMKDGE FHKDGDFAPV SWDKAFDVMA EKWKAALKKN GPTGVGMFGS 150
GQWTVMEGYA AVKLMKAGFR SNNIDPNARH CMASAVGAFM RTFGIDEPMG 200
CYDDFEHADA FVLWGSNMAE MHPVLWTRIT DRRLSHPHVK VNVLSTYYHR 250
SFELADTGYI FEPQSDLAIA NFIANYIIQN DAVNWEFVNK HTNFKQATTD 300
IGYGLRDNDP LQKAAANPNS GKMTDISFED YKASVAEYTV EKASEMSGVS 350
QDDLIKLAKQ YADPNIKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIS 400
TPGNSPFSLT GQPSACGTAR EVGTFSHRLP ADMVVANPKH RKIAEDIWKL 450
PEGTIPPKPG KHAVAQDRAL KDGTINAYWV MCNNNMQAGP NINTERLPGY 500
RNPDNFIVCS DPYPTATAQA SDLILPTAMW IEKEGAYGNA ERRTQAWYQQ 550
VKTVGEAKSD LWQIMEFSKR FTVEEVWGEE LLAKAPEYRG KTMYDVLYKN 600
GNVDAFPLSE AQELNDDAQA QGFYVQKGLF EEYAAFGRDH GHDLAPYDTY 650
HKVRGLRWPV VDGKETLWRF KEGSDPYAKK GSGWDFYGKP DGKALIINAP 700
YEAPPEVPSD EYDMWLCTGR VLEHWHTGTM TRRVPELYKA VPDALCYIHP 750
ADAKARGLRR GDEVLIENKR GEVRVRVETR GRNRPPQGLV FVPFFDARIL 800
INKLILDATD PLSKQTDYKK CPVKITKVS 829
Length:829
Mass (Da):92,614
Last modified:February 10, 2009 - v1
Checksum:i0CA48A08E83D13C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM954973 Genomic DNA. Translation: CAV26209.1.
RefSeqiYP_002395192.1. NC_011744.2.

Genome annotation databases

EnsemblBacteriaiCAV26209; CAV26209; VS_II0598.
GeneIDi7137902.
KEGGivsp:VS_II0598.
PATRICi20149040. VBIVibSpl48387_0579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM954973 Genomic DNA. Translation: CAV26209.1 .
RefSeqi YP_002395192.1. NC_011744.2.

3D structure databases

ProteinModelPortali B7VRL0.
SMRi B7VRL0. Positions 41-827.
ModBasei Search...

Protein-protein interaction databases

STRINGi 575788.VS_II0598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAV26209 ; CAV26209 ; VS_II0598 .
GeneIDi 7137902.
KEGGi vsp:VS_II0598.
PATRICi 20149040. VBIVibSpl48387_0579.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi EYRGKTM.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci VSPL575788:GH64-3680-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Vibrio splendidus str. LGP32 complete genome."
    Mazel D., Le Roux F.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LGP32.

Entry informationi

Entry nameiNAPA_VIBSL
AccessioniPrimary (citable) accession number: B7VRL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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