B7VKI9 (B7VKI9_VIBSL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022

Gene names

Name:	gltX HAMAP MF_00022
Ordered Locus Names:	VS_0769

Organism

Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]

Taxonomic identifier

575788 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022 RuleBase RU003489
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 RuleBase RU003489 EMBL CAV17750.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	9 – 19	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B7VKI9 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 88305138929A139F	FASTA	475	53,270
	10 20 30 40 50 60 MTVKTRFAPS PTGYLHVGGA RTALYSWLFA KNQGGEFVLR IEDTDLERNS QEAVDAILEG 70 80 90 100 110 120 MQWMGMEWDE GPYYQSKRFD RYNEMVDKLL AEDKAFKCYA SKELLDEIRA EQEENKEMAR 130 140 150 160 170 180 YDANHPKIVA ANEAAKEGDA CVIRFRNPKE GSVVFDDQIR GRIEISNSQL DDLIIRRTDG 190 200 210 220 230 240 APTYNFVVVV DDWDMGITHV VRGEDHINNT PRQINIYEAL GAPVPTFAHC AMILGDDGAK 250 260 270 280 290 300 LSKRHGAVSV MQYRDEGYLP NALNNYLVRL GWSHGDQEIF SQEEMIEFFS LNAISKSASA 310 320 330 340 350 360 FNTDKLLWLN NHYIKTSEPE YVAKYLQWHL DAQKIDTTNG PAITEVITLV GERCNTLIEL 370 380 390 400 410 420 AEQSRYFYED FSEFEAGAAK KHLRGVAKGP LELALAKVEA LEDFTTANIK DGVIAAVCEE 430 440 450 460 470 LEVGMGKIGM PLRVAVTGGG QSPSVDAVME LVGKERVIAR IKMALEFIAE REANA

References

[1]	"Vibrio splendidus str. LGP32 complete genome." Mazel D., Le Roux F. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM954972 Genomic DNA. Translation: CAV17750.1.
RefSeq	YP_002416392.1. NC_011753.2.
3D structure databases
ProteinModelPortal	B7VKI9.
ModBase	Search...
Protein-protein interaction databases
STRING	B7VKI9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7160369.
GenomeReviews	Gene locus VS_0769 in contig FM954972_GR.
KEGG	vsp:VS_0769.
PATRIC	20152216. VBIVibSpl48387_2099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B7VKI9_VIBSL

Accession

Primary (citable) accession number: B7VKI9

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)