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B7VKF7 (SYL_VIBSL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:VS_0718
OrganismVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]
Taxonomic identifier575788 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199233

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7VKF7 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: B3F6DA3F0E43E9A9

FASTA85896,921
        10         20         30         40         50         60 
MQEQYNPQEI EQKVQQHWDD SETFVVSEDP NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRFQRLQG KNVMQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WKREFATCTP EYYRWEQEFF TKLYEQGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKKIPQ WFIKITEYAQ ELLDDLDNLE GWPEMVKTMQ RNWIGRSEGV ELSFAVNGEE 

       250        260        270        280        290        300 
APLEVYTTRP DTLMGVSYVG IAAGHPLAEK ASKNNPELAA FVEECRNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMDTGLTAI HPLNGRVVPV YVANFVLMDY GTGAVMAVPA HDQRDYEFAT KYGIDIIPVI 

       370        380        390        400        410        420 
KPEDGSELDV SEAAYTEKGV LFDSGEFDGL AFQEAFDAIA AKLEAEGKGK KTVNFRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMVTTEDG EVHPVPADQL PVILPEDVVM DGVTSPIKAD KSWAETTFNG 

       490        500        510        520        530        540 
EPALRETDTF DTFMESSWYY ARYCSPQADD ILDPEKANYW LPVDQYVGGI EHACMHLLYS 

       550        560        570        580        590        600 
RFFHKLLRDA GYVTSDEPFK QLLCQGMVLA DAFYHENEKG TKEWIAPTDV TVERDGKGRI 

       610        620        630        640        650        660 
EKAVDDQGRE VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP ADMTLEWQES 

       670        680        690        700        710        720 
GVEGANRFLK RVWKLVHAHS SKGAAETVDA SALSGNQKAL RRDIHKTIAK VTDDIGRRQT 

       730        740        750        760        770        780 
FNTAIAAIME LMNKLAKAPQ ESVQDRAILD EALKAVVRML YPMTPHISYE MWIALGESNV 

       790        800        810        820        830        840 
DSATWPTFDE KALVEDEKTI VVMINGKLRA KLTVAADATE EQVRELGLND ENAKKFLDGL 

       850 
TIRKVIFVPG KLLNIVAN 

« Hide

References

[1]"Vibrio splendidus str. LGP32 complete genome."
Mazel D., Le Roux F.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LGP32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM954972 Genomic DNA. Translation: CAV17713.1.
RefSeqYP_002416360.1. NC_011753.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING575788.VS_0718.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAV17713; CAV17713; VS_0718.
GeneID7160337.
KEGGvsp:VS_0718.
PATRIC20152114. VBIVibSpl48387_2066.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycVSPL575788:GH64-692-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VIBSL
AccessionPrimary (citable) accession number: B7VKF7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries