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B7VJ87

- SYI_VIBTL

UniProt

B7VJ87 - SYI_VIBTL

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Vibrio tasmaniensis (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761Aminoacyl-adenylateUniRule annotation
Binding sitei620 – 6201ATPUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi935 – 9351ZincUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciVSPL575788:GH64-517-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:VS_0536
OrganismiVibrio tasmaniensis (strain LGP32) (Vibrio splendidus (strain Mel32))
Taxonomic identifieri575788 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000009100: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Isoleucine--tRNA ligasePRO_1000189215Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi575788.VS_0536.

Structurei

3D structure databases

ProteinModelPortaliB7VJ87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi617 – 6215"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7VJ87-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKDTLNL PETGFPMRGN LANREPEMLK RWYKEDLYGE IRKAKKGKKS
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YIPGWDCHGL
110 120 130 140 150
PIELMVEKKK GKPGQKISAA EFREECRKYA AGQVEGQKES FKRLGIMGEW
160 170 180 190 200
DKPYRTMDFG TEANIIRSLG KIADKGHLLK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYKDKVSPSI DVKFTAADEA ALLEKFTLAE GHAGQGEISI VIWTTTPWTL
260 270 280 290 300
PANRAVCLRD DLEYVLIQVE ANGDQPAQRI VVASELAKDV MDRAGIEHFH
310 320 330 340 350
NLGFATGADL ELSQFNHPFY NFTVPAVLGD HVTTDSGTGV VHTAPGHGQE
360 370 380 390 400
DFVVGKKYNL EIANPVGSNG VYLPDTELFA GQHVFKANDS VLEVLKEKGA
410 420 430 440 450
LLHHHAYEHS YPHCWRHKTP IIFRATPQWF ISMDQAGLRA KALESTKSVE
460 470 480 490 500
WMPEWGQSRI EGMIEGRPEW CISRQRTWGV PIALFVHKET SELHPDSPAL
510 520 530 540 550
IEKVAKLVEE KGIQAWWDVD AAELMGAEDA DKYEKVLDTL DVWFDSGVTH
560 570 580 590 600
FSVVDSREEY NFPNEERTHS ADLYLEGSDQ HRGWFQSSLI SSIAMKDEAP
610 620 630 640 650
YKQVLTHGFV VDGNGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT
660 670 680 690 700
NEVAVSDEIL KRSADAYRRI RNTARFFLAN LNGFNPETDL VPAEEMVALD
710 720 730 740 750
RWAVGRAQAA QEEIVKAYGE YNTHGVTQRL MQFCSIEMGS FYLDVIKDRQ
760 770 780 790 800
YTAKQGSHAQ RSCQTALYYI VEALVRWMAP IMSFTADEIW NEMPGERDTF
810 820 830 840 850
VFTGEWFEGL FGLADDEELS NEFWTEIQSV RGAVNKLLED ARKEKTIGGA
860 870 880 890 900
LQAEVTLYAD DALAAKINKL EDELRFVLIT SAAVVKPLSD KSDTAQATDV
910 920 930 940 950
EGLYVEVAAT EAEKCDRCWH HTPDVGTIEG HEKICGRCVS NIDGEGEVRK

FA
Length:952
Mass (Da):106,624
Last modified:February 10, 2009 - v1
Checksum:i845A0CA06B4F3C80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM954972 Genomic DNA. Translation: CAV17541.1.
RefSeqiYP_002416191.1. NC_011753.2.

Genome annotation databases

EnsemblBacteriaiCAV17541; CAV17541; VS_0536.
GeneIDi7160168.
KEGGivsp:VS_0536.
PATRICi20151772. VBIVibSpl48387_1900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM954972 Genomic DNA. Translation: CAV17541.1 .
RefSeqi YP_002416191.1. NC_011753.2.

3D structure databases

ProteinModelPortali B7VJ87.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 575788.VS_0536.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAV17541 ; CAV17541 ; VS_0536 .
GeneIDi 7160168.
KEGGi vsp:VS_0536.
PATRICi 20151772. VBIVibSpl48387_1900.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci VSPL575788:GH64-517-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Vibrio splendidus str. LGP32 complete genome."
    Mazel D., Le Roux F.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LGP32.

Entry informationi

Entry nameiSYI_VIBTL
AccessioniPrimary (citable) accession number: B7VJ87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3