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B7VIS0

- GLND_VIBTL

UniProt

B7VIS0 - GLND_VIBTL

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio tasmaniensis (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciVSPL575788:GH64-2314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:VS_2355
OrganismiVibrio tasmaniensis (strain LGP32) (Vibrio splendidus (strain Mel32))
Taxonomic identifieri575788 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000009100: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000132531Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi575788.VS_2355.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini451 – 584134HDUniRule annotationAdd
BLAST
Domaini693 – 77785ACT 1UniRule annotationAdd
BLAST
Domaini800 – 87374ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseAdd
BLAST
Regioni333 – 692360Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7VIS0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPYQCPITFN DEQLEICELK NQLEIFTQYQ KSEFLNHHPV TDLVLLRSEY
60 70 80 90 100
MDLLLNRLWE HFGFSKLPHI ALVAVGGYGR GELHPLSDID ILIVSQRTLP
110 120 130 140 150
SALGEKVSQF ITLLWDLKLE VGHAVRTIAE CIEIGSDDLT VATNLQESRL
160 170 180 190 200
LCGSEDTFQE LKLKIHSDSF WPSETFYKAK IQEQRERHAR YHDTTYNLEP
210 220 230 240 250
DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSKYGFLTDA EYRELVECQD
260 270 280 290 300
FLWRVRFALH IELRRYDNRL TFAHQAQVAE HLGYTGEGNR GVEMMMKEFY
310 320 330 340 350
RTLRRVAELN KMLLKLFDQA IINGGQTQEA EILDNDFQRR GSLIEARKPA
360 370 380 390 400
LFQARPETIL DMFIHIANDS TIEGVSPPTL RQLRTARRRL NRFLHTIPEA
410 420 430 440 450
RDKFMDLVRH PNALHKAFSL MHKLGVLSAY LPQWSQIVGQ MQFDLFHVYT
460 470 480 490 500
VDEHSIRLLK HINRFSQVEN HDKHPICCEV YPRVQKKELL ILAAIFHDIG
510 520 530 540 550
KGRGGDHSEI GAVEAYSFCI EHGLSKPEAK QVAWLVQNHL LMSVTAQRRD
560 570 580 590 600
IYDPDVITEF AKKVRDEESL ELLVCLTVAD ICATNPELWN SWKRTLLAEL
610 620 630 640 650
FHSTQRALRR GLENPVDVRD RIRHNQQMAS ALLRKEGFSA REIEVLWQRF
660 670 680 690 700
KADYFLRHTH TQIAWHCEHL LRLEDPSQPL VLISKKATRG GTEVFVYCKD
710 720 730 740 750
QAALFATVVA ELDRRNFNVH DAQVMVSKDG HVLDTFIVLD QHGEAIDEAR
760 770 780 790 800
HKAVAKHLTH VLADGRPTKI KTRRTPRNLQ HFKVKTRVEF LPTKSKKRTL
810 820 830 840 850
MELRALDTPG LLAQVGATFA ELDINLHGAK ITTIGERAED LFILTSDAGG
860 870
RLSEEQEQAL RERLTEHVSE LAP
Length:873
Mass (Da):100,638
Last modified:February 10, 2009 - v1
Checksum:iF87E5B8C89D7356D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM954972 Genomic DNA. Translation: CAV19516.1.
RefSeqiWP_012604606.1. NC_011753.2.
YP_002417941.1. NC_011753.2.

Genome annotation databases

EnsemblBacteriaiCAV19516; CAV19516; VS_2355.
GeneIDi7161894.
KEGGivsp:VS_2355.
PATRICi20155290. VBIVibSpl48387_3618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM954972 Genomic DNA. Translation: CAV19516.1 .
RefSeqi WP_012604606.1. NC_011753.2.
YP_002417941.1. NC_011753.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 575788.VS_2355.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAV19516 ; CAV19516 ; VS_2355 .
GeneIDi 7161894.
KEGGi vsp:VS_2355.
PATRICi 20155290. VBIVibSpl48387_3618.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci VSPL575788:GH64-2314-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Vibrio splendidus str. LGP32 complete genome."
    Mazel D., Le Roux F.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LGP32.

Entry informationi

Entry nameiGLND_VIBTL
AccessioniPrimary (citable) accession number: B7VIS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3