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B7VIS0

- GLND_VIBSL

UniProt

B7VIS0 - GLND_VIBSL

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciVSPL575788:GH64-2314-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:VS_2355
    OrganismiVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
    Taxonomic identifieri575788 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000009100: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000132531Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi575788.VS_2355.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini451 – 584134HDUniRule annotationAdd
    BLAST
    Domaini693 – 77785ACT 1UniRule annotationAdd
    BLAST
    Domaini800 – 87374ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 332332UridylyltransferaseAdd
    BLAST
    Regioni333 – 692360Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7VIS0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPYQCPITFN DEQLEICELK NQLEIFTQYQ KSEFLNHHPV TDLVLLRSEY    50
    MDLLLNRLWE HFGFSKLPHI ALVAVGGYGR GELHPLSDID ILIVSQRTLP 100
    SALGEKVSQF ITLLWDLKLE VGHAVRTIAE CIEIGSDDLT VATNLQESRL 150
    LCGSEDTFQE LKLKIHSDSF WPSETFYKAK IQEQRERHAR YHDTTYNLEP 200
    DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSKYGFLTDA EYRELVECQD 250
    FLWRVRFALH IELRRYDNRL TFAHQAQVAE HLGYTGEGNR GVEMMMKEFY 300
    RTLRRVAELN KMLLKLFDQA IINGGQTQEA EILDNDFQRR GSLIEARKPA 350
    LFQARPETIL DMFIHIANDS TIEGVSPPTL RQLRTARRRL NRFLHTIPEA 400
    RDKFMDLVRH PNALHKAFSL MHKLGVLSAY LPQWSQIVGQ MQFDLFHVYT 450
    VDEHSIRLLK HINRFSQVEN HDKHPICCEV YPRVQKKELL ILAAIFHDIG 500
    KGRGGDHSEI GAVEAYSFCI EHGLSKPEAK QVAWLVQNHL LMSVTAQRRD 550
    IYDPDVITEF AKKVRDEESL ELLVCLTVAD ICATNPELWN SWKRTLLAEL 600
    FHSTQRALRR GLENPVDVRD RIRHNQQMAS ALLRKEGFSA REIEVLWQRF 650
    KADYFLRHTH TQIAWHCEHL LRLEDPSQPL VLISKKATRG GTEVFVYCKD 700
    QAALFATVVA ELDRRNFNVH DAQVMVSKDG HVLDTFIVLD QHGEAIDEAR 750
    HKAVAKHLTH VLADGRPTKI KTRRTPRNLQ HFKVKTRVEF LPTKSKKRTL 800
    MELRALDTPG LLAQVGATFA ELDINLHGAK ITTIGERAED LFILTSDAGG 850
    RLSEEQEQAL RERLTEHVSE LAP 873
    Length:873
    Mass (Da):100,638
    Last modified:February 10, 2009 - v1
    Checksum:iF87E5B8C89D7356D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM954972 Genomic DNA. Translation: CAV19516.1.
    RefSeqiYP_002417941.1. NC_011753.2.

    Genome annotation databases

    EnsemblBacteriaiCAV19516; CAV19516; VS_2355.
    GeneIDi7161894.
    KEGGivsp:VS_2355.
    PATRICi20155290. VBIVibSpl48387_3618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM954972 Genomic DNA. Translation: CAV19516.1 .
    RefSeqi YP_002417941.1. NC_011753.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 575788.VS_2355.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAV19516 ; CAV19516 ; VS_2355 .
    GeneIDi 7161894.
    KEGGi vsp:VS_2355.
    PATRICi 20155290. VBIVibSpl48387_3618.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci VSPL575788:GH64-2314-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vibrio splendidus str. LGP32 complete genome."
      Mazel D., Le Roux F.
      Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LGP32.

    Entry informationi

    Entry nameiGLND_VIBSL
    AccessioniPrimary (citable) accession number: B7VIS0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3