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B7VIS0

- GLND_VIBSL

UniProt

B7VIS0 - GLND_VIBSL

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, VS_2355
Organism
Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciVSPL575788:GH64-2314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:VS_2355
OrganismiVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
Taxonomic identifieri575788 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000009100: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000132531Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi575788.VS_2355.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini451 – 584134HDAdd
BLAST
Domaini693 – 77785ACT 1Add
BLAST
Domaini800 – 87374ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseUniRule annotationAdd
BLAST
Regioni333 – 692360Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7VIS0-1 [UniParc]FASTAAdd to Basket

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MPYQCPITFN DEQLEICELK NQLEIFTQYQ KSEFLNHHPV TDLVLLRSEY    50
MDLLLNRLWE HFGFSKLPHI ALVAVGGYGR GELHPLSDID ILIVSQRTLP 100
SALGEKVSQF ITLLWDLKLE VGHAVRTIAE CIEIGSDDLT VATNLQESRL 150
LCGSEDTFQE LKLKIHSDSF WPSETFYKAK IQEQRERHAR YHDTTYNLEP 200
DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSKYGFLTDA EYRELVECQD 250
FLWRVRFALH IELRRYDNRL TFAHQAQVAE HLGYTGEGNR GVEMMMKEFY 300
RTLRRVAELN KMLLKLFDQA IINGGQTQEA EILDNDFQRR GSLIEARKPA 350
LFQARPETIL DMFIHIANDS TIEGVSPPTL RQLRTARRRL NRFLHTIPEA 400
RDKFMDLVRH PNALHKAFSL MHKLGVLSAY LPQWSQIVGQ MQFDLFHVYT 450
VDEHSIRLLK HINRFSQVEN HDKHPICCEV YPRVQKKELL ILAAIFHDIG 500
KGRGGDHSEI GAVEAYSFCI EHGLSKPEAK QVAWLVQNHL LMSVTAQRRD 550
IYDPDVITEF AKKVRDEESL ELLVCLTVAD ICATNPELWN SWKRTLLAEL 600
FHSTQRALRR GLENPVDVRD RIRHNQQMAS ALLRKEGFSA REIEVLWQRF 650
KADYFLRHTH TQIAWHCEHL LRLEDPSQPL VLISKKATRG GTEVFVYCKD 700
QAALFATVVA ELDRRNFNVH DAQVMVSKDG HVLDTFIVLD QHGEAIDEAR 750
HKAVAKHLTH VLADGRPTKI KTRRTPRNLQ HFKVKTRVEF LPTKSKKRTL 800
MELRALDTPG LLAQVGATFA ELDINLHGAK ITTIGERAED LFILTSDAGG 850
RLSEEQEQAL RERLTEHVSE LAP 873
Length:873
Mass (Da):100,638
Last modified:February 10, 2009 - v1
Checksum:iF87E5B8C89D7356D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM954972 Genomic DNA. Translation: CAV19516.1.
RefSeqiYP_002417941.1. NC_011753.2.

Genome annotation databases

EnsemblBacteriaiCAV19516; CAV19516; VS_2355.
GeneIDi7161894.
KEGGivsp:VS_2355.
PATRICi20155290. VBIVibSpl48387_3618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM954972 Genomic DNA. Translation: CAV19516.1 .
RefSeqi YP_002417941.1. NC_011753.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 575788.VS_2355.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAV19516 ; CAV19516 ; VS_2355 .
GeneIDi 7161894.
KEGGi vsp:VS_2355.
PATRICi 20155290. VBIVibSpl48387_3618.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci VSPL575788:GH64-2314-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Vibrio splendidus str. LGP32 complete genome."
    Mazel D., Le Roux F.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LGP32.

Entry informationi

Entry nameiGLND_VIBSL
AccessioniPrimary (citable) accession number: B7VIS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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