B7VI86 (CYSH_VIBSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	VS_0301

Organism

Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]

Taxonomic identifier

575788 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite By similarity. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000117933

Sequences

	Sequence		Length	Mass (Da)
	B7VI86 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 6CEEB0C13121E45C	FASTA	258	29,522
	10 20 30 40 50 60 MHNSVASKLK LAELLALTKT EQILRLGQIN AELEQLTALE RVKWALENLE GTHVVSSSFG 70 80 90 100 110 120 IQAALMLHLV TQAKSDIPVI LTDTGYLFPE TYRFIDELSQ KLTLNLQVFR AQQSSNWQEA 130 140 150 160 170 180 QYGKLWDQGI EGIEKYNKLN KVEPMRRALD ELEAGTWFSG LRREQSQSRA NLPILSIQNG 190 200 210 220 230 240 VFKFLPVIDW TNKDVHYYLE EHGLSYHPLR EQGYLSVGDT HTTKKWEPGM TEEETRFNGL 250 KRECGLHEDD GEQYGSGI

References

[1]	"Vibrio splendidus str. LGP32 complete genome." Mazel D., Le Roux F. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LGP32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM954972 Genomic DNA. Translation: CAV17323.1.
RefSeq	YP_002415975.1. NC_011753.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B7VI86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7159952.
GenomeReviews	Gene locus VS_0301 in contig FM954972_GR.
KEGG	vsp:VS_0301.
PATRIC	20151320. VBIVibSpl48387_1680.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	TNFRPYE.
ProtClustDB	PRK02090.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBSL

Accession

Primary (citable) accession number: B7VI86

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program