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B7VI85

- CYSI_VIBSL

UniProt

B7VI85 - CYSI_VIBSL

Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

    Catalytic activityi

    H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

    Cofactori

    Binds 1 siroheme per subunit.UniRule annotation
    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi438 – 4381Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi444 – 4441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi484 – 4841Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi488 – 4881Iron (siroheme axial ligand)UniRule annotation
    Metal bindingi488 – 4881Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. heme binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. NADP binding Source: InterPro
    5. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
    3. sulfate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciVSPL575788:GH64-289-MONOMER.
    UniPathwayiUPA00140; UER00207.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
    Short name:
    SiR-HPUniRule annotation
    Short name:
    SiRHPUniRule annotation
    Gene namesi
    Name:cysIUniRule annotation
    Ordered Locus Names:VS_0300
    OrganismiVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32))
    Taxonomic identifieri575788 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000009100: Chromosome 1

    Subcellular locationi

    GO - Cellular componenti

    1. sulfite reductase complex (NADPH) Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575Sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000388526Add
    BLAST

    Interactioni

    Subunit structurei

    Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

    Protein-protein interaction databases

    STRINGi575788.VS_0300.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0155.
    HOGENOMiHOG000218418.
    KOiK00381.
    OMAiELHQEAY.
    OrthoDBiEOG6W9XFB.

    Family and domain databases

    Gene3Di3.90.480.10. 2 hits.
    HAMAPiMF_01540. CysI.
    InterProiIPR011786. CysI.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    [Graphical view]
    PfamiPF01077. NIR_SIR. 2 hits.
    PF03460. NIR_SIR_ferr. 2 hits.
    [Graphical view]
    PRINTSiPR00397. SIROHAEM.
    SUPFAMiSSF55124. SSF55124. 2 hits.
    TIGRFAMsiTIGR02041. CysI. 1 hit.
    PROSITEiPS00365. NIR_SIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7VI85-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKQVIEQEV LGQVLGPLAD NERLKRESKN LRGTIEQDLE DRITGGFTAD    50
    NFQLIRFHGM YQQDDRDIRN ERTKQKLEPL HNVMLRARMP GGIITPKQWL 100
    AIDKFADEST SYGSIRLTTR QTFQFHGVLK PNIKLMHQTL NSIGIDSIAT 150
    AGDVNRNVLC TTNPVESELH QEAYEWAKKI SEHLLPKTRA YAEIWLDGEK 200
    LATTDEEPIL GSNYLPRKFK TTVVIPPQND VDVHANDLNF IAIAKDGKLV 250
    GFNVLVGGGL AMTHGDTSTY ARKADDFGFV PLEKTLDVAA AVVTTQRDWG 300
    NRSNRKNAKT KYTLDRVGID VFKAEVEKRA GVEFAESRPY EFTGRGDRIG 350
    WAEGIDGKHH LALFIENGRL LDFPGKALKT GVAEIAKIHK GDFRMTANQN 400
    LIVAGVLKSQ KAQIEKLARQ YGLMDDAVSE QRKNSMACVA FPTCPLAMAE 450
    AERFLPEFVT DVEDILKKHG LPEEDNIILR ITGCPNGCGR AMLAELGLVG 500
    KAPGRYNMHL GGNKAGTRIP KMYKENITSA QILEEIDSLV GRWATERTDN 550
    EGFGDFTIRA GIIEEVIISK RDLHA 575
    Length:575
    Mass (Da):64,122
    Last modified:February 10, 2009 - v1
    Checksum:i60F39AC340FFFCEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM954972 Genomic DNA. Translation: CAV17322.1.
    RefSeqiWP_012603121.1. NC_011753.2.
    YP_002415974.1. NC_011753.2.

    Genome annotation databases

    EnsemblBacteriaiCAV17322; CAV17322; VS_0300.
    GeneIDi7159951.
    KEGGivsp:VS_0300.
    PATRICi20151318. VBIVibSpl48387_1679.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM954972 Genomic DNA. Translation: CAV17322.1 .
    RefSeqi WP_012603121.1. NC_011753.2.
    YP_002415974.1. NC_011753.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 575788.VS_0300.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAV17322 ; CAV17322 ; VS_0300 .
    GeneIDi 7159951.
    KEGGi vsp:VS_0300.
    PATRICi 20151318. VBIVibSpl48387_1679.

    Phylogenomic databases

    eggNOGi COG0155.
    HOGENOMi HOG000218418.
    KOi K00381.
    OMAi ELHQEAY.
    OrthoDBi EOG6W9XFB.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00207 .
    BioCyci VSPL575788:GH64-289-MONOMER.

    Family and domain databases

    Gene3Di 3.90.480.10. 2 hits.
    HAMAPi MF_01540. CysI.
    InterProi IPR011786. CysI.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    [Graphical view ]
    Pfami PF01077. NIR_SIR. 2 hits.
    PF03460. NIR_SIR_ferr. 2 hits.
    [Graphical view ]
    PRINTSi PR00397. SIROHAEM.
    SUPFAMi SSF55124. SSF55124. 2 hits.
    TIGRFAMsi TIGR02041. CysI. 1 hit.
    PROSITEi PS00365. NIR_SIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vibrio splendidus str. LGP32 complete genome."
      Mazel D., Le Roux F.
      Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LGP32.

    Entry informationi

    Entry nameiCYSI_VIBSL
    AccessioniPrimary (citable) accession number: B7VI85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3