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Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Vibrio tasmaniensis (strain LGP32) (Vibrio splendidus (strain Mel32))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • sirohemeUniRule annotationNote: Binds 1 siroheme per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathway:ihydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] hemoprotein beta-component (cysI), Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi438 – 4381Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi444 – 4441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi484 – 4841Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi488 – 4881Iron (siroheme axial ligand)UniRule annotation
Metal bindingi488 – 4881Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciVSPL575788:GH64-289-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-HPUniRule annotation
Short name:
SiRHPUniRule annotation
Gene namesi
Name:cysIUniRule annotation
Ordered Locus Names:VS_0300
OrganismiVibrio tasmaniensis (strain LGP32) (Vibrio splendidus (strain Mel32))
Taxonomic identifieri575788 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000009100 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000388526Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi575788.VS_0300.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0155.
HOGENOMiHOG000218418.
KOiK00381.
OrthoDBiEOG6W9XFB.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7VI85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQVIEQEV LGQVLGPLAD NERLKRESKN LRGTIEQDLE DRITGGFTAD
60 70 80 90 100
NFQLIRFHGM YQQDDRDIRN ERTKQKLEPL HNVMLRARMP GGIITPKQWL
110 120 130 140 150
AIDKFADEST SYGSIRLTTR QTFQFHGVLK PNIKLMHQTL NSIGIDSIAT
160 170 180 190 200
AGDVNRNVLC TTNPVESELH QEAYEWAKKI SEHLLPKTRA YAEIWLDGEK
210 220 230 240 250
LATTDEEPIL GSNYLPRKFK TTVVIPPQND VDVHANDLNF IAIAKDGKLV
260 270 280 290 300
GFNVLVGGGL AMTHGDTSTY ARKADDFGFV PLEKTLDVAA AVVTTQRDWG
310 320 330 340 350
NRSNRKNAKT KYTLDRVGID VFKAEVEKRA GVEFAESRPY EFTGRGDRIG
360 370 380 390 400
WAEGIDGKHH LALFIENGRL LDFPGKALKT GVAEIAKIHK GDFRMTANQN
410 420 430 440 450
LIVAGVLKSQ KAQIEKLARQ YGLMDDAVSE QRKNSMACVA FPTCPLAMAE
460 470 480 490 500
AERFLPEFVT DVEDILKKHG LPEEDNIILR ITGCPNGCGR AMLAELGLVG
510 520 530 540 550
KAPGRYNMHL GGNKAGTRIP KMYKENITSA QILEEIDSLV GRWATERTDN
560 570
EGFGDFTIRA GIIEEVIISK RDLHA
Length:575
Mass (Da):64,122
Last modified:February 10, 2009 - v1
Checksum:i60F39AC340FFFCEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM954972 Genomic DNA. Translation: CAV17322.1.
RefSeqiWP_012603121.1. NC_011753.2.

Genome annotation databases

EnsemblBacteriaiCAV17322; CAV17322; VS_0300.
GeneIDi7159951.
KEGGivsp:VS_0300.
PATRICi20151318. VBIVibSpl48387_1679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM954972 Genomic DNA. Translation: CAV17322.1.
RefSeqiWP_012603121.1. NC_011753.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi575788.VS_0300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAV17322; CAV17322; VS_0300.
GeneIDi7159951.
KEGGivsp:VS_0300.
PATRICi20151318. VBIVibSpl48387_1679.

Phylogenomic databases

eggNOGiCOG0155.
HOGENOMiHOG000218418.
KOiK00381.
OrthoDBiEOG6W9XFB.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciVSPL575788:GH64-289-MONOMER.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Vibrio splendidus str. LGP32 complete genome."
    Mazel D., Le Roux F.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LGP32.

Entry informationi

Entry nameiCYSI_VIBTL
AccessioniPrimary (citable) accession number: B7VI85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: February 10, 2009
Last modified: July 22, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.