B7VI85 (CYSI_VIBSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-component
Short name=SiR-HP
Short name=SiRHP
EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	VS_0300

Organism

Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]

Taxonomic identifier

575788 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio splendidus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP-Rule MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP-Rule MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP-Rule MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP-Rule MF_01540		PRO_0000388526
Sites
	Metal binding	438	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	444	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	484	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	488	1	Iron (siroheme axial ligand) By similarity
	Metal binding	488	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7VI85 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 60F39AC340FFFCEF	FASTA	575	64,122
	10 20 30 40 50 60 MSKQVIEQEV LGQVLGPLAD NERLKRESKN LRGTIEQDLE DRITGGFTAD NFQLIRFHGM 70 80 90 100 110 120 YQQDDRDIRN ERTKQKLEPL HNVMLRARMP GGIITPKQWL AIDKFADEST SYGSIRLTTR 130 140 150 160 170 180 QTFQFHGVLK PNIKLMHQTL NSIGIDSIAT AGDVNRNVLC TTNPVESELH QEAYEWAKKI 190 200 210 220 230 240 SEHLLPKTRA YAEIWLDGEK LATTDEEPIL GSNYLPRKFK TTVVIPPQND VDVHANDLNF 250 260 270 280 290 300 IAIAKDGKLV GFNVLVGGGL AMTHGDTSTY ARKADDFGFV PLEKTLDVAA AVVTTQRDWG 310 320 330 340 350 360 NRSNRKNAKT KYTLDRVGID VFKAEVEKRA GVEFAESRPY EFTGRGDRIG WAEGIDGKHH 370 380 390 400 410 420 LALFIENGRL LDFPGKALKT GVAEIAKIHK GDFRMTANQN LIVAGVLKSQ KAQIEKLARQ 430 440 450 460 470 480 YGLMDDAVSE QRKNSMACVA FPTCPLAMAE AERFLPEFVT DVEDILKKHG LPEEDNIILR 490 500 510 520 530 540 ITGCPNGCGR AMLAELGLVG KAPGRYNMHL GGNKAGTRIP KMYKENITSA QILEEIDSLV 550 560 570 GRWATERTDN EGFGDFTIRA GIIEEVIISK RDLHA

References

[1]	"Vibrio splendidus str. LGP32 complete genome." Mazel D., Le Roux F. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LGP32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM954972 Genomic DNA. Translation: CAV17322.1.
RefSeq	YP_002415974.1. NC_011753.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	575788.VS_0300.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAV17322; CAV17322; VS_0300.
GeneID	7159951.
KEGG	vsp:VS_0300.
PATRIC	20151318. VBIVibSpl48387_1679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HOG000218418.
KO	K00381.
OMA	RNVMAPA.
ProtClustDB	PRK13504.
Enzyme and pathway databases
UniPathway	UPA00140; UER00207.
Family and domain databases
Gene3D	3.90.480.10. 2 hits.
HAMAP	MF_01540. CysI.
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 2 hits.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_VIBSL

Accession

Primary (citable) accession number: B7VI85

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program