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B7VI85 (CYSI_VIBSL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sulfite reductase [NADPH] hemoprotein beta-component

Short name=SiR-HP
Short name=SiRHP
EC=1.8.1.2
Gene names
Name:cysI
Ordered Locus Names:VS_0300
OrganismVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]
Taxonomic identifier575788 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP-Rule MF_01540

Catalytic activity

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP-Rule MF_01540

Cofactor

Binds 1 siroheme per subunit By similarity. HAMAP-Rule MF_01540

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01540

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP-Rule MF_01540

Subunit structure

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575Sulfite reductase [NADPH] hemoprotein beta-component HAMAP-Rule MF_01540
PRO_0000388526

Sites

Metal binding4381Iron-sulfur (4Fe-4S) By similarity
Metal binding4441Iron-sulfur (4Fe-4S) By similarity
Metal binding4841Iron-sulfur (4Fe-4S) By similarity
Metal binding4881Iron (siroheme axial ligand) By similarity
Metal binding4881Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B7VI85 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 60F39AC340FFFCEF

FASTA57564,122
        10         20         30         40         50         60 
MSKQVIEQEV LGQVLGPLAD NERLKRESKN LRGTIEQDLE DRITGGFTAD NFQLIRFHGM 

        70         80         90        100        110        120 
YQQDDRDIRN ERTKQKLEPL HNVMLRARMP GGIITPKQWL AIDKFADEST SYGSIRLTTR 

       130        140        150        160        170        180 
QTFQFHGVLK PNIKLMHQTL NSIGIDSIAT AGDVNRNVLC TTNPVESELH QEAYEWAKKI 

       190        200        210        220        230        240 
SEHLLPKTRA YAEIWLDGEK LATTDEEPIL GSNYLPRKFK TTVVIPPQND VDVHANDLNF 

       250        260        270        280        290        300 
IAIAKDGKLV GFNVLVGGGL AMTHGDTSTY ARKADDFGFV PLEKTLDVAA AVVTTQRDWG 

       310        320        330        340        350        360 
NRSNRKNAKT KYTLDRVGID VFKAEVEKRA GVEFAESRPY EFTGRGDRIG WAEGIDGKHH 

       370        380        390        400        410        420 
LALFIENGRL LDFPGKALKT GVAEIAKIHK GDFRMTANQN LIVAGVLKSQ KAQIEKLARQ 

       430        440        450        460        470        480 
YGLMDDAVSE QRKNSMACVA FPTCPLAMAE AERFLPEFVT DVEDILKKHG LPEEDNIILR 

       490        500        510        520        530        540 
ITGCPNGCGR AMLAELGLVG KAPGRYNMHL GGNKAGTRIP KMYKENITSA QILEEIDSLV 

       550        560        570 
GRWATERTDN EGFGDFTIRA GIIEEVIISK RDLHA 

« Hide

References

[1]"Vibrio splendidus str. LGP32 complete genome."
Mazel D., Le Roux F.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LGP32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM954972 Genomic DNA. Translation: CAV17322.1.
RefSeqYP_002415974.1. NC_011753.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING575788.VS_0300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAV17322; CAV17322; VS_0300.
GeneID7159951.
KEGGvsp:VS_0300.
PATRIC20151318. VBIVibSpl48387_1679.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0155.
HOGENOMHOG000218418.
KOK00381.
OMAELHQEAY.
OrthoDBEOG6W9XFB.

Enzyme and pathway databases

BioCycVSPL575788:GH64-289-MONOMER.
UniPathwayUPA00140; UER00207.

Family and domain databases

Gene3D3.90.480.10. 2 hits.
HAMAPMF_01540. CysI.
InterProIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSPR00397. SIROHAEM.
SUPFAMSSF55124. SSF55124. 2 hits.
TIGRFAMsTIGR02041. CysI. 1 hit.
PROSITEPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSI_VIBSL
AccessionPrimary (citable) accession number: B7VI85
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways