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B7VGL4 (FADB_VIBSL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fatty acid oxidation complex subunit alpha

Including the following 2 domains:

  1. Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
    EC=4.2.1.17
    EC=5.1.2.3
    EC=5.3.3.8
  2. 3-hydroxyacyl-CoA dehydrogenase
    EC=1.1.1.35
Gene names
Name:fadB
Ordered Locus Names:VS_0041
OrganismVibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) [Complete proteome] [HAMAP]
Taxonomic identifier575788 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length723 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate By similarity. HAMAP-Rule MF_01621

Catalytic activity

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP-Rule MF_01621

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP-Rule MF_01621

(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP-Rule MF_01621

(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. HAMAP-Rule MF_01621

Pathway

Lipid metabolism; fatty acid beta-oxidation. HAMAP-Rule MF_01621

Subunit structure

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA) By similarity.

Sequence similarities

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 723723Fatty acid oxidation complex subunit alpha HAMAP-Rule MF_01621
PRO_1000186059

Regions

Nucleotide binding401 – 4033NAD By similarity
Nucleotide binding428 – 4303NAD By similarity
Region1 – 189189Enoyl-CoA hydratase/isomerase By similarity
Region311 – 7234133-hydroxyacyl-CoA dehydrogenase By similarity

Sites

Active site4511For 3-hydroxyacyl-CoA dehydrogenase activity By similarity
Binding site2961Substrate By similarity
Binding site3251NAD; via amide nitrogen By similarity
Binding site3441NAD By similarity
Binding site4081NAD By similarity
Binding site4541NAD By similarity
Binding site5011Substrate By similarity
Binding site6611Substrate By similarity
Site1191Important for catalytic activity By similarity
Site1391Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B7VGL4 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 9268B0DFE76F707D

FASTA72378,363
        10         20         30         40         50         60 
MIYQANTLQV KELQDGIAEL SFCAPASVNK LDLATLESLD KALDALNAHA GLRGLILTSN 

        70         80         90        100        110        120 
KDAFIVGADI TEFLGLFAKP EAELDEWLRF ANSIFSKLED LPVPTLSMMR GHALGGGCEC 

       130        140        150        160        170        180 
VLATDFRIGD KTTSIGLPET KLGIMPGFGG CVRLPRVIGA DSAMEIITQG KACRADEALK 

       190        200        210        220        230        240 
VGLLDAIVET DQLLESAINT VSLAANEKLD WQLRRKQKTS ALSLSKLEAM MSFTMAKGLV 

       250        260        270        280        290        300 
AQKAGPHYPA PITSVIAIEE AARSDRDAAL DIERKHFVKL AKSEEAKALV GLFLNDQYIK 

       310        320        330        340        350        360 
GLAKHAGKSA NKATERAAVL GAGIMGGGIA YQSALKGVPV MMKDIAQASL ELGMNEASKL 

       370        380        390        400        410        420 
LNKRLSRGRL DGFKMAGILS SITPSLHYAG VEQSDVIVEA VVENPKVKAA VLSEVEGLVG 

       430        440        450        460        470        480 
EDTVLTSNTS TIPINLLAKS LKRPENFCGM HFFNPVHRMP LVEIIRGEHT SEETINRVVA 

       490        500        510        520        530        540 
YAAKMGKSPI VVNDCPGFFV NRVLFPYFGG FSMLLRDGAD FTKIDKVMER KFGWPMGPAY 

       550        560        570        580        590        600 
LLDVVGLDTA HHAQAVMAQG FPERMGKEGR DAIDALYVAE KYGQKNGSGF YTYSVDKRGR 

       610        620        630        640        650        660 
PKKTFSEDIL PILADVCQQP QDFDDQTIIQ RVMIPMINEV VLCLEEGIIA TPQEADMALV 

       670        680        690        700        710        720 
YGLGFPPFRG GVFRYLDSVG IGNFVEMAKS YQDLGAMYQV PQLLLDMAAK GESFYDGQQA 


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References

[1]"Vibrio splendidus str. LGP32 complete genome."
Mazel D., Le Roux F.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LGP32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM954972 Genomic DNA. Translation: CAV17097.1.
RefSeqYP_002415749.1. NC_011753.2.

3D structure databases

ProteinModelPortalB7VGL4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING575788.VS_0041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAV17097; CAV17097; VS_0041.
GeneID7159737.
KEGGvsp:VS_0041.
PATRIC20150830. VBIVibSpl48387_1455.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1250.
HOGENOMHOG000261344.
KOK01825.
OMANPIVVND.
OrthoDBEOG6M9F0M.
ProtClustDBPRK11730.

Enzyme and pathway databases

BioCycVSPL575788:GH64-41-MONOMER.
UniPathwayUPA00659.

Family and domain databases

Gene3D1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
HAMAPMF_01621. FadB.
InterProIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 2 hits.
TIGRFAMsTIGR02437. FadB. 1 hit.
PROSITEPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFADB_VIBSL
AccessionPrimary (citable) accession number: B7VGL4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways