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B7VB85 (SYQ_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:PLES_35351
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000199102

Regions

Motif35 – 4511"HIGH" region HAMAP-Rule MF_00126
Motif269 – 2735"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2721ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7VB85 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 2851975F982926F0

FASTA55662,957
        10         20         30         40         50         60 
MSKPETTAAP NFLRQIVQAD LDAGKHAKIV TRFPPEPNGY LHIGHAKSIC LNFGLAQEFA 

        70         80         90        100        110        120 
GDCHLRFDDT NPAKEDQEYI DAIEADIKWL GFQWSGEVCY ASNYFDQLHA WAIELIKAGK 

       130        140        150        160        170        180 
AFVCDLGPEE MREYRGTLTE PGRNSPYRDR SVEENLDLFA RMKAGEFPDG ARSLRAKIDM 

       190        200        210        220        230        240 
GSPNMNLRDP ILYRIRHAHH HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP 

       250        260        270        280        290        300 
LYEWFLANLP VPAQPRQYEF SRLNLNYTVT SKRKLKQLVD EGHVSGWDDP RMSTLSGYRR 

       310        320        330        340        350        360 
RGYTPESIRN FCEMIGVNRA SGVVDIGMLE FSIRDHLDAT APRAMCVLKP LKVVITNYPE 

       370        380        390        400        410        420 
GQVENLELPR HPKEDMGVRV LPFGRELFID AGDFEEVPPA GYKRLIPGGE VRLRGSYVIR 

       430        440        450        460        470        480 
ADEAIKDADG NIVELRCSYD PDTLGKNPEG RKVKGVIHWV PAEGSVECEV RLYDRLFRSA 

       490        500        510        520        530        540 
NPEKAEEGGS FLDNINADSL QVLTGCRAEP SLGQANPEDR FQFEREGYFV ADLKDSRPGK 

       550 
PVFNRTVTLR DSWGQG 

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM209186 Genomic DNA. Translation: CAW28262.1.
RefSeqYP_002441121.1. NC_011770.1.

3D structure databases

ProteinModelPortalB7VB85.
SMRB7VB85. Positions 11-552.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557722.PLES_35351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW28262; CAW28262; PLES_35351.
GeneID7179819.
KEGGpag:PLES_35351.
PATRIC19816833. VBIPseAer113719_3642.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycPAER557722:GHJW-3583-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_PSEA8
AccessionPrimary (citable) accession number: B7VB85
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries