Reviewed, UniProtKB/Swiss-Prot B7VA96 (HGD_PSEA8)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	PLES_33141

Organism

Pseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]

Taxonomic identifier

557722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_1000119845
Sites
	Metal binding	330	1	Iron By similarity
	Metal binding	336	1	Iron By similarity
	Metal binding	366	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7VA96-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 44B17EC2A0811E2A	FASTA	432	47,841
	10 20 30 40 50 60 MNLDSTALAY QSGFGNEFSS EALPGALPVG QNSPQKAPYG LYAELLSGTA FTMARSEARR 70 80 90 100 110 120 TWLYRITPSA KHPPFRRLER QIAGAELDAP TPNRLRWDPL ALPEQPTDFL DGLLRMAANA 130 140 150 160 170 180 PGDKPAGVSI YQYLANRSME RCFYDADGEL LLVPQLGRLR LCTELGALQV EPLEIAVIPR 190 200 210 220 230 240 GMKFRVELLD GEARGYIAEN HGAPLRLPDL GPIGSNGLAN PRDFLTPVAR YEDSRQPLQL 250 260 270 280 290 300 VQKYLGELWA CELDHSPLDV VAWHGNNVPY KYDLRRFNTI GTVSFDHPDP SIFTVLTSPT 310 320 330 340 350 360 SVPGLANIDF VIFPPRWMVA ENTFRPPWFH RNLMNEFMGL IQGAYDAKAG GFVPGGASLH 370 380 390 400 410 420 SCMSAHGPDA ESCDKAIAAD LKPHRIDQTM AFMFETSQVL RPSRAALETP ALQNDYDACW 430 ASLVSTFNPQ RR

References

[1]

"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa." Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C. Genome Res. 19:12-23(2009) [PubMed: 19047519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM209186 Genomic DNA. Translation: CAW28041.1.
RefSeq	YP_002440900.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7177639.
GenomeReviews	Gene locus PLES_33141 in contig FM209186_GR.
KEGG	pag:PLES_33141.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEA8

Accession

Primary (citable) accession number: B7VA96

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)