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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei287 – 2871Proton acceptorUniRule annotation
Metal bindingi330 – 3301IronUniRule annotation
Metal bindingi336 – 3361IronUniRule annotation
Binding sitei345 – 3451homogentisateUniRule annotation
Metal bindingi366 – 3661IronUniRule annotation
Binding sitei366 – 3661homogentisateUniRule annotation

GO - Molecular functioni

  1. homogentisate 1,2-dioxygenase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-phenylalanine catabolic process Source: UniProtKB-HAMAP
  2. tyrosine catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciPAER557722:GHJW-3357-MONOMER.
UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:PLES_33141
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001527: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Homogentisate 1,2-dioxygenasePRO_1000119845Add
BLAST

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi557722.PLES_33141.

Structurei

3D structure databases

ProteinModelPortaliB7VA96.
SMRiB7VA96. Positions 8-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

B7VA96-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLDSTALAY QSGFGNEFSS EALPGALPVG QNSPQKAPYG LYAELLSGTA
60 70 80 90 100
FTMARSEARR TWLYRITPSA KHPPFRRLER QIAGAELDAP TPNRLRWDPL
110 120 130 140 150
ALPEQPTDFL DGLLRMAANA PGDKPAGVSI YQYLANRSME RCFYDADGEL
160 170 180 190 200
LLVPQLGRLR LCTELGALQV EPLEIAVIPR GMKFRVELLD GEARGYIAEN
210 220 230 240 250
HGAPLRLPDL GPIGSNGLAN PRDFLTPVAR YEDSRQPLQL VQKYLGELWA
260 270 280 290 300
CELDHSPLDV VAWHGNNVPY KYDLRRFNTI GTVSFDHPDP SIFTVLTSPT
310 320 330 340 350
SVPGLANIDF VIFPPRWMVA ENTFRPPWFH RNLMNEFMGL IQGAYDAKAG
360 370 380 390 400
GFVPGGASLH SCMSAHGPDA ESCDKAIAAD LKPHRIDQTM AFMFETSQVL
410 420 430
RPSRAALETP ALQNDYDACW ASLVSTFNPQ RR
Length:432
Mass (Da):47,841
Last modified:February 10, 2009 - v1
Checksum:i44B17EC2A0811E2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW28041.1.
RefSeqiYP_002440900.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW28041; CAW28041; PLES_33141.
GeneIDi7177639.
KEGGipag:PLES_33141.
PATRICi19816369. VBIPseAer113719_3415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW28041.1.
RefSeqiYP_002440900.1. NC_011770.1.

3D structure databases

ProteinModelPortaliB7VA96.
SMRiB7VA96. Positions 8-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi557722.PLES_33141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAW28041; CAW28041; PLES_33141.
GeneIDi7177639.
KEGGipag:PLES_33141.
PATRICi19816369. VBIPseAer113719_3415.

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.
BioCyciPAER557722:GHJW-3357-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58.

Entry informationi

Entry nameiHGD_PSEA8
AccessioniPrimary (citable) accession number: B7VA96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.