B7V9V5 (B7V9V5_PSEA8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
EC=2.4.1.18 HAMAP-Rule MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP-Rule MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP-Rule MF_00685
Glycogen branching enzyme HAMAP-Rule MF_00685

Gene names

Name:	glgB HAMAP-Rule MF_00685 EMBL CAW27900.1
Ordered Locus Names:	PLES_31731

Organism

Pseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]

Taxonomic identifier

557722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685 SAAS SAAS006048
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685 SAAS SAAS006048
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP-Rule MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP-Rule MF_00685 SAAS SAAS006048 Glycogen metabolism HAMAP-Rule MF_00685
Molecular function	Glycosyltransferase HAMAP-Rule MF_00685 SAAS SAAS006048 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	412	1	Nucleophile By similarity HAMAP-Rule MF_00685
	Active site	465	1	Proton donor By similarity HAMAP-Rule MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B7V9V5 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: C31303D6D5F929F4	FASTA	732	82,563
	10 20 30 40 50 60 MSEPMSERER WQLELDKLQR GLQGDPFAFL GPQRDPGGEG GVLRAYLPGA QRVELLDEDG 70 80 90 100 110 120 ATLAELEQSD PGSGLFQRHL ERLPPRYRLR VHWPDGVQES EDPYAFGPLL GELDLYLFAE 130 140 150 160 170 180 GNHRQLASCL GAQLTRHEGV EGVRFAVWAP NAVRVSVVGD FNGWDGRRHP MRRRYPSGVW 190 200 210 220 230 240 ELFVPRLGEG ELYKYELQGH DGLLPLKADP LALACETPPG TASKTCAALR HEWRDQDWLA 250 260 270 280 290 300 RRAERQGYAA PLSIYEVHAG SWRHRDGRPP HWSELAEELI PYVRQLGFTH IELMPVMEHP 310 320 330 340 350 360 FGGSWGYQPL GLFAPTARYG TPEDFAAFVD ACHQAGIGVI LDWVPAHFPT DAHGLGRFDG 370 380 390 400 410 420 TALYEYEHPF EGFHQDWDTY IYNLGRSEVH GFMLASALHW LRTYHVDGLR VDAVASMLYR 430 440 450 460 470 480 DYSRKEGEWL PNRHGGRENL EAIDFLHHLN QVVASETPGA LVIAEESTAW PGVSRPVAEG 490 500 510 520 530 540 GLGFSHKWNM GWMHDSLAYI GEDPLHRRYH HHQLTFGLLY AFSEHFILPI SHDEVVHGKH 550 560 570 580 590 600 SLLDKMPGDR WQKFANLRLY LSFMWSHPGK KLLFMGCEFG QWREWNHDGE LDWYLLRYPE 610 620 630 640 650 660 HQGVQDLVAA LNRLYRELPA LHARDGEALG FEWLIGDDQA NSVYAWLRHA AGEPSLLAVH 670 680 690 700 710 720 NFTPVPRQGY RIGVPQGGDW DVLLNSDAQA FAGSGAGSQG RVSSESCGAH GQAQSLVLDL 730 PPLGTLLLRP AG

References

[1]

"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa." Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C. Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LESB58.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM209186 Genomic DNA. Translation: CAW27900.1.
RefSeq	YP_002440762.1. NC_011770.1.
3D structure databases
ProteinModelPortal	B7V9V5.
SMR	B7V9V5. Positions 123-728.
ModBase	Search...
Protein-protein interaction databases
STRING	557722.PLES_31731.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAW27900; CAW27900; PLES_31731.
GeneID	7177523.
KEGG	pag:PLES_31731.
PATRIC	19816056. VBIPseAer113719_3260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	QGFHPEW.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	PAER557722:GHJW-4900-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B7V9V5_PSEA8

Accession

Primary (citable) accession number: B7V9V5

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)