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B7V9C9

- SYL_PSEA8

UniProt

B7V9C9 - SYL_PSEA8

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei635 – 6351ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPAER557722:GHJW-1003-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:PLES_09881
    OrganismiPseudomonas aeruginosa (strain LESB58)
    Taxonomic identifieri557722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000001527: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 873873Leucine--tRNA ligasePRO_1000199219Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi557722.PLES_09881.

    Structurei

    3D structure databases

    ProteinModelPortaliB7V9C9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi632 – 6365"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7V9C9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHEQYQPLEI ETQAQNYWKE HQSFLVRELP DKEKFYCLSM FPYPSGKLHM    50
    GHVRNYTIGD VISRYHRMQG RNVLQPMGWD AFGMPAENAA MKNNVAPAAW 100
    TYDNIAYMKS QLDSLGLAID WSREVTTCKP DYYRWEQWLF TRLFEKGVIY 150
    RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAE 200
    ELLESLDNLP GWPEQVKTMQ RNWIGKSRGM EIGFPYDQAS IGHAGQLKVF 250
    TTRPDTLMGA TYVAVAAEHP LATQAAQNDP QLQAFIDECK RGGVAEADIA 300
    TQEKKGMATS LFVEHPLTGD KLPVWVANYV LMNYGEGAVM AVPGHDERDF 350
    EFANKYGLPI RQVIAKVEGD NDFESSVWKE WYGAKDESVL TVNSGKYDNL 400
    GYQAAFDAIG ADLEAKGLGQ ARTQFRLRDW GISRQRYWGC PIPIIHCEAC 450
    GDVPVPADQL PVVLPEDVVP DGSGSPLAKM PEFYECNCPK CGQPAKRETD 500
    TMDTFVESSW YFARYACPQF EGGMLDRKAA DYWLPVDQYI GGIEHAILHL 550
    LYARFFHKLM RDEGLVGSDE PFKNLLTQGM VVADTYYRTT ANGGKDWFNP 600
    ADVEVERDAK AKVVGARLKS DGQPVEIGGT EKMSKSKNNG VDPQSMIDQY 650
    GADTCRLFMM FASPPDMSLE WSDAGVEGAN RFLRRVWRLA HAHVSAGLPG 700
    ALDVATLSDA QKQVRRAIHL AIRQASQDVG QHHKFNTAIA AVMTLMNVLE 750
    KAPNQDAQDR ALIQEGLETV VLLLAPITPH ICHVLWGQLG HAEAVIDARW 800
    PSVDESALVQ DTLQLVVQVN GKLRGHIDVA ASASREDVEA AARANENVLR 850
    FTEGLSIRKV IVVPGKLVNI VAN 873
    Length:873
    Mass (Da):97,678
    Last modified:February 10, 2009 - v1
    Checksum:i4CBC246D189F69D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM209186 Genomic DNA. Translation: CAW25715.1.
    RefSeqiWP_012613615.1. NC_011770.1.
    YP_002438594.1. NC_011770.1.

    Genome annotation databases

    EnsemblBacteriaiCAW25715; CAW25715; PLES_09881.
    GeneIDi7175858.
    KEGGipag:PLES_09881.
    PATRICi19811532. VBIPseAer113719_1027.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM209186 Genomic DNA. Translation: CAW25715.1 .
    RefSeqi WP_012613615.1. NC_011770.1.
    YP_002438594.1. NC_011770.1.

    3D structure databases

    ProteinModelPortali B7V9C9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 557722.PLES_09881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAW25715 ; CAW25715 ; PLES_09881 .
    GeneIDi 7175858.
    KEGGi pag:PLES_09881.
    PATRICi 19811532. VBIPseAer113719_1027.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci PAER557722:GHJW-1003-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
      Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
      Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LESB58.

    Entry informationi

    Entry nameiSYL_PSEA8
    AccessioniPrimary (citable) accession number: B7V9C9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3