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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13SubstrateUniRule annotation1
Binding sitei35SubstrateUniRule annotation1
Active sitei64Proton donorUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei182SubstrateUniRule annotation1
Binding sitei191SubstrateUniRule annotation1
Binding sitei211Substrate; via amide nitrogenUniRule annotation1
Binding sitei212SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:PLES_21881
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001385481 – 232Orotidine 5'-phosphate decarboxylaseAdd BLAST232

Proteomic databases

PRIDEiB7V800

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7V800
SMRiB7V800
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 71Substrate bindingUniRule annotation10

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226070
KOiK01591
OMAiNFKIFLD

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01200_B OMPdecase_type1_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014732 OMPdecase
IPR018089 OMPdecase_AS
IPR001754 OMPdeCOase_dom
IPR011060 RibuloseP-bd_barrel
PANTHERiPTHR32119:SF2 PTHR32119:SF2, 1 hit
PfamiView protein in Pfam
PF00215 OMPdecase, 1 hit
SMARTiView protein in SMART
SM00934 OMPdecase, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01740 pyrF, 1 hit
PROSITEiView protein in PROSITE
PS00156 OMPDECASE, 1 hit

Sequencei

Sequence statusi: Complete.

B7V800-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSACQSPIIV ALDFPTREAA LALADQLDPK LCRVKVGKEL FTSCAAGIVE
60 70 80 90 100
TLRGKGFEVF LDLKFHDIPN TTAMAVKAAA EMGVWMVNVH CSGGLRMMAA
110 120 130 140 150
CRETLEAFSG PRPLLIGVTV LTSMEREDLA GIGLDIEPQE QVLRLAALAQ
160 170 180 190 200
KAGMDGLVCS AQEAPALKAA HPGLQLVTPG IRPAGSAQDD QRRILTPRQA
210 220 230
LDAGSDYLVI GRPISQAADP AKALAAIVAE LG
Length:232
Mass (Da):24,394
Last modified:February 10, 2009 - v1
Checksum:iEE7218004DA944B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA Translation: CAW26915.1
RefSeqiWP_003090975.1, NC_011770.1

Genome annotation databases

EnsemblBacteriaiCAW26915; CAW26915; PLES_21881
KEGGipag:PLES_21881

Similar proteinsi

Entry informationi

Entry nameiPYRF_PSEA8
AccessioniPrimary (citable) accession number: B7V800
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health