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B7V6A5 (B7V6A5_PSEA8) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL CAW25453.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:PLES_07261
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. SAAS SAAS005905 HAMAP-Rule MF_00047

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain138 – 332195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding164 – 21754ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2861Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3011Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
B7V6A5 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: A66A99C9B74958E7

FASTA34636,476
        10         20         30         40         50         60 
MGQDKLKVAV LFGGSSEERD VSIASGAQVI QALRSAGHQV LAVDTASGLL GAEEERRLLA 

        70         80         90        100        110        120 
SKVKEVPPDS DSLAIIRSGK QSLLSAGELA GVDVFFLALH GGTGEDGTLQ ALLDAGGFAY 

       130        140        150        160        170        180 
TGSGHLASAM AMDKDVAKRL FLAAGVETAS WLMAPASEEA VREQLGFPLV VKPNSQGSTV 

       190        200        210        220        230        240 
GLSIVHSQAE LQPAIELAGR YGDEVMLERF VAGREVTVGV LDDQALPVGE ILLGGQEVFD 

       250        260        270        280        290        300 
YEHKYQAGAV REVFPADLPP AIAAEAQRLA LKVHRALKLS GYSRTDFRLD EQGRLWCLEV 

       310        320        330        340 
NTLPGMTATS LLPQAAAAAG IGFAELCERI CRLGIERCKG ARKARS

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM209186 Genomic DNA. Translation: CAW25453.1.
RefSeqYP_002438334.1. NC_011770.1.

3D structure databases

ProteinModelPortalB7V6A5.
ModBaseSearch...

Protein-protein interaction databases

STRING557722.PLES_07261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW25453; CAW25453; PLES_07261.
GeneID7175648.
KEGGpag:PLES_07261.
PATRIC19810987. VBIPseAer113719_0756.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAVEDASCD.
ProtClustDBCLSK868521.

Enzyme and pathway databases

BioCycPAER557722:GHJW-4687-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7V6A5_PSEA8
AccessionPrimary (citable) accession number: B7V6A5
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info