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Protein

Elongation factor Tu

Gene

tufB

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotationImported

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER557722:GHJW-662-MONOMER.
PAER557722:GHJW-677-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufBImported
Synonyms:tufUniRule annotation, tufAImported
Ordered Locus Names:PLES_06511Imported, PLES_06631Imported
OrganismiPseudomonas aeruginosa (strain LESB58)Imported
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001527 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiB7V630.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557722.PLES_06631.

Structurei

3D structure databases

ProteinModelPortaliB7V630.
SMRiB7V630. Positions 11-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7V630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEKFERNK PHVNVGTIGH VDHGKTTLTA ALTKVCSDTW GGSARAFDQI
60 70 80 90 100
DNAPEEKARG ITINTSHVEY DSAVRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVCSAAD GPMPQTREHI LLSRQVGVPY IVVFLNKADM VDDAELLELV
160 170 180 190 200
EMEVRDLLNT YDFPGDDTPI IIGSALMALE GKDDNGIGVS AVQKLVETLD
210 220 230 240 250
SYIPEPVRAI DQPFLMPIED VFSISGRGTV VTGRVERGII KVQEEVEIVG
260 270 280 290 300
IKATTKTTCT GVEMFRKLLD EGRAGENVGI LLRGTKREDV ERGQVLAKPG
310 320 330 340 350
TIKPHTKFEC EVYVLSKEEG GRHTPFFKGY RPQFYFRTTD VTGNCELPEG
360 370 380 390
VEMVMPGDNI KMVVTLIAPI AMEDGLRFAI REGGRTVGAG VVAKIIE
Length:397
Mass (Da):43,370
Last modified:February 10, 2009 - v1
Checksum:iA019D5BF8EBAB942
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW25378.1.
FM209186 Genomic DNA. Translation: CAW25390.1.
RefSeqiYP_002438259.1. NC_011770.1.
YP_002438271.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW25378; CAW25378; PLES_06511.
CAW25390; CAW25390; PLES_06631.
KEGGipag:PLES_06511.
pag:PLES_06631.
PATRICi19810830. VBIPseAer113719_0679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW25378.1.
FM209186 Genomic DNA. Translation: CAW25390.1.
RefSeqiYP_002438259.1. NC_011770.1.
YP_002438271.1. NC_011770.1.

3D structure databases

ProteinModelPortaliB7V630.
SMRiB7V630. Positions 11-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi557722.PLES_06631.

Proteomic databases

PRIDEiB7V630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAW25378; CAW25378; PLES_06511.
CAW25390; CAW25390; PLES_06631.
KEGGipag:PLES_06511.
pag:PLES_06631.
PATRICi19810830. VBIPseAer113719_0679.

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Enzyme and pathway databases

BioCyciPAER557722:GHJW-662-MONOMER.
PAER557722:GHJW-677-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E., Brinkman F.S., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58Imported.

Entry informationi

Entry nameiB7V630_PSEA8
AccessioniPrimary (citable) accession number: B7V630
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: April 29, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.