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B7V5R4 (BETB_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenase

Short name=BADH
EC=1.2.1.8
Gene names
Name:betB
Ordered Locus Names:PLES_57681
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid By similarity. HAMAP-Rule MF_00804

Catalytic activity

Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP-Rule MF_00804

Cofactor

Binds 2 potassium ions per subunit By similarity. HAMAP-Rule MF_00804

Pathway

Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP-Rule MF_00804

Subunit structure

Dimer of dimers By similarity. HAMAP-Rule MF_00804

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   LigandMetal-binding
NAD
NADP
Potassium
   Molecular functionOxidoreductase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine betaine biosynthetic process from choline

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionbetaine-aldehyde dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490NAD/NADP-dependent betaine aldehyde dehydrogenase HAMAP-Rule MF_00804
PRO_1000133955

Regions

Nucleotide binding150 – 1534NAD/NADP By similarity
Nucleotide binding176 – 1794NAD/NADP By similarity
Nucleotide binding229 – 2346NAD/NADP By similarity

Sites

Active site1621Charge relay system By similarity
Active site2521Proton acceptor By similarity
Active site4641Charge relay system By similarity
Metal binding261Potassium 1 By similarity
Metal binding271Potassium 1; via carbonyl oxygen By similarity
Metal binding931Potassium 1 By similarity
Metal binding1801Potassium 1; via carbonyl oxygen By similarity
Metal binding2461Potassium 2; via carbonyl oxygen By similarity
Metal binding4571Potassium 2; via carbonyl oxygen By similarity
Metal binding4601Potassium 2; via carbonyl oxygen By similarity
Binding site2091NAD/NADP; via amide nitrogen By similarity
Binding site2861NAD/NADP By similarity
Binding site3871NAD/NADP By similarity
Site2481Seems to be a necessary countercharge to the potassium cations By similarity

Amino acid modifications

Modified residue2861Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
B7V5R4 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: F48249A085E3A970

FASTA49053,332
        10         20         30         40         50         60 
MARFEEQKLY IGGRYVEASS GATFETINPA NGEVLAKVQR ASREDVERAV QSAVEGQKVW 

        70         80         90        100        110        120 
AAMTAMQRSR ILRRAVDILR ERNDELAALE TLDTGKPLAE TRSVDIVTGA DVLEYYAGLV 

       130        140        150        160        170        180 
PAIEGEQIPL RETSFVYTRR EPLGVVAGIG AWNYPVQIAL WKSAPALAAG NAMIFKPSEV 

       190        200        210        220        230        240 
TPLTALKLAE IYTEAGVPDG VFNVLTGSGR EVGQWLTEHP LIEKISFTGG TSTGKKVMAS 

       250        260        270        280        290        300 
ASSSSLKEVT MELGGKSPLI IFPDADLDRA ADIAVMANFF SSGQVCTNGT RVFIHRSQQA 

       310        320        330        340        350        360 
RFEAKVLERV QRIRLGDPQD ENTNFGPLVS FPHMESVLGY IESGKAQKAR LLCGGERVTD 

       370        380        390        400        410        420 
GAFGKGAYVA PTVFTDCRDD MTIVREEIFG PVMSILVYDD EDEAIRRAND TEYGLAAGVV 

       430        440        450        460        470        480 
TQDLARAHRA IHRLEAGICW INTWGESPAE MPVGGYKQSG VGRENGLTTL AHYTRIKSVQ 

       490 
VELGDYASVF 

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM209186 Genomic DNA. Translation: CAW30522.1.
RefSeqYP_002443346.1. NC_011770.1.

3D structure databases

ProteinModelPortalB7V5R4.
SMRB7V5R4. Positions 2-490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557722.PLES_57681.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW30522; CAW30522; PLES_57681.
GeneID7179672.
KEGGpag:PLES_57681.
PATRIC19821487. VBIPseAer113719_5930.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHOG000271505.
KOK00130.
OMACTDEMTI.
OrthoDBEOG6BS8QW.

Enzyme and pathway databases

BioCycPAER557722:GHJW-5864-MONOMER.
UniPathwayUPA00529; UER00386.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPMF_00804. BADH.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01804. BADH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBETB_PSEA8
AccessionPrimary (citable) accession number: B7V5R4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways