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B7V5L2

- DUT_PSEA8

UniProt

B7V5L2 - DUT_PSEA8

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Protein
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Gene
dut, PLES_57161
Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831Substrate By similarity
Binding sitei97 – 971Substrate; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPAER557722:GHJW-5807-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:PLES_57161
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001527: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 151151Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation
PRO_1000117572Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi557722.PLES_57161.

Structurei

3D structure databases

ProteinModelPortaliB7V5L2.
SMRiB7V5L2. Positions 1-135.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 723Substrate binding By similarity
Regioni87 – 893Substrate binding By similarity

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028968.
KOiK01520.
OMAiSIYIGDP.
OrthoDBiEOG689HXK.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

B7V5L2-1 [UniParc]FASTAAdd to Basket

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MHSLQAKILD PRLGSDFPLP QYATPGSAGL DLRAMLKEDS VLGPGQTLLI    50
PTGLSIYIAD PGLAALVLPR SGLGHKHGIV LGNLVGLIDS DYQGELMVSC 100
WNRGESPFTI AVGERIAQLV LVPVVQAHFE LVEAFDESQR GAGGFGHSGS 150
H 151
Length:151
Mass (Da):15,920
Last modified:February 10, 2009 - v1
Checksum:i7F87AD5DEF031A48
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM209186 Genomic DNA. Translation: CAW30470.1.
RefSeqiYP_002443294.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW30470; CAW30470; PLES_57161.
GeneIDi7179743.
KEGGipag:PLES_57161.
PATRICi19821369. VBIPseAer113719_5876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM209186 Genomic DNA. Translation: CAW30470.1 .
RefSeqi YP_002443294.1. NC_011770.1.

3D structure databases

ProteinModelPortali B7V5L2.
SMRi B7V5L2. Positions 1-135.
ModBasei Search...

Protein-protein interaction databases

STRINGi 557722.PLES_57161.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAW30470 ; CAW30470 ; PLES_57161 .
GeneIDi 7179743.
KEGGi pag:PLES_57161.
PATRICi 19821369. VBIPseAer113719_5876.

Phylogenomic databases

eggNOGi COG0756.
HOGENOMi HOG000028968.
KOi K01520.
OMAi SIYIGDP.
OrthoDBi EOG689HXK.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
BioCyci PAER557722:GHJW-5807-MONOMER.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
HAMAPi MF_00116. dUTPase_bact.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58.

Entry informationi

Entry nameiDUT_PSEA8
AccessioniPrimary (citable) accession number: B7V5L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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