Reviewed, UniProtKB/Swiss-Prot B7V594 (ARGA_PSEA8)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	PLES_55981

Organism

Pseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]

Taxonomic identifier

557722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000137052
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	B7V594-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 725FAD778FD44A4E	FASTA	432	47,859
	10 20 30 40 50 60 MPDYVNWLRH ASPYINSHRD RTFVVMLPGE GVEHPNFGNI VHDLVLLHSL GARLVLVHGS 70 80 90 100 110 120 RPQIEARLAA RGLAPRYHRD LRVTDAPTLE CVIDAVGSLR IAIEARLSMD MAASPMQGAR 130 140 150 160 170 180 LRVAGGNLVT ARPIGVVEGV DYHHTGEVRR IDRKGIGRLL DERSIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVAMR AAIDLEAEKL ILYGAEQGLL DASGKLVREL RPQQVPAHLQ RLGNSYQAEL 250 260 270 280 290 300 LDAAAQACRA GVKRSHIVSY TEDGALLSEL FTRTGNGTLV AQEQFEQLRE AGIEDVGGLI 310 320 330 340 350 360 ELIRPLEEQG ILVRRSREVL EREIEQFSIV EREGLIIACA ALYPIADSEA GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIEERAR GLGLKTLFVL TTRTAHWFRE RGFQPSSVER LPAARASLYN 430 FQRNSQVFEK SL

References

[1]

"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa." Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C. Genome Res. 19:12-23(2009) [PubMed: 19047519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM209186 Genomic DNA. Translation: CAW30352.1.
RefSeq	YP_002443176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7180098.
GenomeReviews	Gene locus PLES_55981 in contig FM209186_GR.
KEGG	pag:PLES_55981.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSEA8

Accession

Primary (citable) accession number: B7V594

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)