Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B7V467

- MASZ_PSEA8

UniProt

B7V467 - MASZ_PSEA8

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi432 – 4321MagnesiumUniRule annotation
Binding sitei432 – 4321GlyoxylateUniRule annotation
Metal bindingi460 – 4601MagnesiumUniRule annotation
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei631 – 6311Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPAER557722:GHJW-479-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:PLES_04781
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001527: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GPRO_1000130894Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557722.PLES_04781.

Structurei

3D structure databases

ProteinModelPortaliB7V467.
SMRiB7V467. Positions 2-722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni457 – 4604Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

B7V467-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN
60 70 80 90 100
KALLAKRDEL QAKIDGWHQA RAGQAHDAAA YKAFLEEIGY LLPEAEDFQA
110 120 130 140 150
GTQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDVISE
160 170 180 190 200
EGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLESGSHVDA TSYSVKNGAL
210 220 230 240 250
VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF EIQIDPSSPV
260 270 280 290 300
GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL
310 320 330 340 350
AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA
360 370 380 390 400
ILDKDGNEVP EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG
410 420 430 440 450
PEEAAFTNEL FGRVEDVLGL PRNTLKVGIM DEERRTTVNL KACIKAAKDR
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKSEKWIGAY ENNNVDVGLA
510 520 530 540 550
TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV PSPTAATLHA
560 570 580 590 600
LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV
660 670 680 690 700
ISQEQVVESL KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG
710 720
TRQPNGYTEP VLHRRRREFK AKNGL
Length:725
Mass (Da):78,632
Last modified:February 10, 2009 - v1
Checksum:iCEC503219946E5CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW25205.1.
RefSeqiYP_002438086.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW25205; CAW25205; PLES_04781.
GeneIDi7175500.
KEGGipag:PLES_04781.
PATRICi19810454. VBIPseAer113719_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW25205.1 .
RefSeqi YP_002438086.1. NC_011770.1.

3D structure databases

ProteinModelPortali B7V467.
SMRi B7V467. Positions 2-722.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557722.PLES_04781.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAW25205 ; CAW25205 ; PLES_04781 .
GeneIDi 7175500.
KEGGi pag:PLES_04781.
PATRICi 19810454. VBIPseAer113719_0500.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci PAER557722:GHJW-479-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58.

Entry informationi

Entry nameiMASZ_PSEA8
AccessioniPrimary (citable) accession number: B7V467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 26, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3