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B7V3T9 (PCKA_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckA
Ordered Locus Names:PLES_55861
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

phosphoenolpyruvate carboxykinase (ATP) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453
PRO_1000125081

Regions

Nucleotide binding220 – 2278ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7V3T9 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: BFE7F109710B124C

FASTA51355,732
        10         20         30         40         50         60 
MTQANNAVYT DISAAQLVEE AIRRGEGELA ANGSLVVRTG HRTGRSPVDR FIVEEPSTKD 

        70         80         90        100        110        120 
AIAWGNINRP FPADKFDALW ARVEAFNNAQ DHFISHVHVG SAEAYYLPVK MTTATAWQNL 

       130        140        150        160        170        180 
FGRCLFIEPE QYNPAGKDEW QVLNVANFEC VPERDGTNSD GCVILNFAQK KVLIAGMRYA 

       190        200        210        220        230        240 
GEMKKAMFSV QNFLLPERDV LPMHCAANIG EAGDVTLFFG LSGTGKTTLS ADESRYLIGD 

       250        260        270        280        290        300 
DEHGWGEGVV FNVEGGCYAK CIDLSEKNEP VIWKAIKFGA VLENVVLDEE RVPNYADDSL 

       310        320        330        340        350        360 
TQNSRAAYPL EHVEKRSEKN LGGEPNAVIF LTCDLTGVLP PVSILNNEQA AYHFLSGYTA 

       370        380        390        400        410        420 
LVGSTEMGSG GGIKSTFSTC FGAPFFPRPA GVYAELLIKR IKAFGSKVYL VNTGWTGGGY 

       430        440        450        460        470        480 
GVGKRFNIPT TRGVIAAIQS GALIGAETEH LDIINLDVPK AVPGVETNLL NPRNTWADKA 

       490        500        510 
AYDEAAKGLA KQFIENFKKF EVSDAIKAAG PQL

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM209186 Genomic DNA. Translation: CAW30340.1.
RefSeqYP_002443164.1. NC_011770.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING557722.PLES_55861.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW30340; CAW30340; PLES_55861.
GeneID7179271.
KEGGpag:PLES_55861.
PATRIC19821099. VBIPseAer113719_5741.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1866.
HOGENOMHOG000271471.
KOK01610.
OMATRCAYPI.
ProtClustDBPRK09344.

Enzyme and pathway databases

BioCycPAER557722:GHJW-5394-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00453. PEPCK_ATP.
InterProIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMsTIGR00224. pckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKA_PSEA8
AccessionPrimary (citable) accession number: B7V3T9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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