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B7V3T9

- PCKA_PSEA8

UniProt

B7V3T9 - PCKA_PSEA8

Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

    Catalytic activityi

    ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451SubstrateUniRule annotation
    Binding sitei179 – 1791SubstrateUniRule annotation
    Metal bindingi185 – 1851ManganeseUniRule annotation
    Binding sitei185 – 1851ATPUniRule annotation
    Binding sitei185 – 1851SubstrateUniRule annotation
    Metal bindingi204 – 2041Manganese; via tele nitrogenUniRule annotation
    Binding sitei204 – 2041ATPUniRule annotation
    Metal bindingi241 – 2411ManganeseUniRule annotation
    Binding sitei269 – 2691ATPUniRule annotation
    Binding sitei305 – 3051ATPUniRule annotation
    Binding sitei305 – 3051SubstrateUniRule annotation
    Binding sitei431 – 4311ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi220 – 2289ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (ATP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPAER557722:GHJW-5677-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
    Short name:
    PCKUniRule annotation
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckAUniRule annotation
    Ordered Locus Names:PLES_55861
    OrganismiPseudomonas aeruginosa (strain LESB58)
    Taxonomic identifieri557722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000001527: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 513513Phosphoenolpyruvate carboxykinase [ATP]PRO_1000125081Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi557722.PLES_55861.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1866.
    HOGENOMiHOG000271471.
    KOiK01610.
    OMAiGADPEHY.
    OrthoDBiEOG6DG2RK.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00453. PEPCK_ATP.
    InterProiIPR001272. PEP_carboxykinase_ATP.
    IPR013035. PEP_carboxykinase_C.
    IPR008210. PEP_carboxykinase_N.
    IPR015994. PEPCK_ATP_CS.
    [Graphical view]
    PfamiPF01293. PEPCK_ATP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    TIGRFAMsiTIGR00224. pckA. 1 hit.
    PROSITEiPS00532. PEPCK_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7V3T9-1 [UniParc]FASTAAdd to Basket

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    MTQANNAVYT DISAAQLVEE AIRRGEGELA ANGSLVVRTG HRTGRSPVDR    50
    FIVEEPSTKD AIAWGNINRP FPADKFDALW ARVEAFNNAQ DHFISHVHVG 100
    SAEAYYLPVK MTTATAWQNL FGRCLFIEPE QYNPAGKDEW QVLNVANFEC 150
    VPERDGTNSD GCVILNFAQK KVLIAGMRYA GEMKKAMFSV QNFLLPERDV 200
    LPMHCAANIG EAGDVTLFFG LSGTGKTTLS ADESRYLIGD DEHGWGEGVV 250
    FNVEGGCYAK CIDLSEKNEP VIWKAIKFGA VLENVVLDEE RVPNYADDSL 300
    TQNSRAAYPL EHVEKRSEKN LGGEPNAVIF LTCDLTGVLP PVSILNNEQA 350
    AYHFLSGYTA LVGSTEMGSG GGIKSTFSTC FGAPFFPRPA GVYAELLIKR 400
    IKAFGSKVYL VNTGWTGGGY GVGKRFNIPT TRGVIAAIQS GALIGAETEH 450
    LDIINLDVPK AVPGVETNLL NPRNTWADKA AYDEAAKGLA KQFIENFKKF 500
    EVSDAIKAAG PQL 513
    Length:513
    Mass (Da):55,732
    Last modified:February 10, 2009 - v1
    Checksum:iBFE7F109710B124C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM209186 Genomic DNA. Translation: CAW30340.1.
    RefSeqiYP_002443164.1. NC_011770.1.

    Genome annotation databases

    EnsemblBacteriaiCAW30340; CAW30340; PLES_55861.
    GeneIDi7179271.
    KEGGipag:PLES_55861.
    PATRICi19821099. VBIPseAer113719_5741.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM209186 Genomic DNA. Translation: CAW30340.1 .
    RefSeqi YP_002443164.1. NC_011770.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 557722.PLES_55861.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAW30340 ; CAW30340 ; PLES_55861 .
    GeneIDi 7179271.
    KEGGi pag:PLES_55861.
    PATRICi 19821099. VBIPseAer113719_5741.

    Phylogenomic databases

    eggNOGi COG1866.
    HOGENOMi HOG000271471.
    KOi K01610.
    OMAi GADPEHY.
    OrthoDBi EOG6DG2RK.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci PAER557722:GHJW-5677-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00453. PEPCK_ATP.
    InterProi IPR001272. PEP_carboxykinase_ATP.
    IPR013035. PEP_carboxykinase_C.
    IPR008210. PEP_carboxykinase_N.
    IPR015994. PEPCK_ATP_CS.
    [Graphical view ]
    Pfami PF01293. PEPCK_ATP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006294. PEP_crbxkin. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    TIGRFAMsi TIGR00224. pckA. 1 hit.
    PROSITEi PS00532. PEPCK_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
      Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
      Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LESB58.

    Entry informationi

    Entry nameiPCKA_PSEA8
    AccessioniPrimary (citable) accession number: B7V3T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3