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B7V3T9

- PCKA_PSEA8

UniProt

B7V3T9 - PCKA_PSEA8

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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451SubstrateUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Metal bindingi185 – 1851ManganeseUniRule annotation
Binding sitei185 – 1851ATPUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation
Metal bindingi204 – 2041Manganese; via tele nitrogenUniRule annotation
Binding sitei204 – 2041ATPUniRule annotation
Metal bindingi241 – 2411ManganeseUniRule annotation
Binding sitei269 – 2691ATPUniRule annotation
Binding sitei305 – 3051ATPUniRule annotation
Binding sitei305 – 3051SubstrateUniRule annotation
Binding sitei431 – 4311ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2289ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (ATP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER557722:GHJW-5677-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:PLES_55861
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001527: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Phosphoenolpyruvate carboxykinase [ATP]PRO_1000125081Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557722.PLES_55861.

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1866.
HOGENOMiHOG000271471.
KOiK01610.
OMAiGADPEHY.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7V3T9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQANNAVYT DISAAQLVEE AIRRGEGELA ANGSLVVRTG HRTGRSPVDR
60 70 80 90 100
FIVEEPSTKD AIAWGNINRP FPADKFDALW ARVEAFNNAQ DHFISHVHVG
110 120 130 140 150
SAEAYYLPVK MTTATAWQNL FGRCLFIEPE QYNPAGKDEW QVLNVANFEC
160 170 180 190 200
VPERDGTNSD GCVILNFAQK KVLIAGMRYA GEMKKAMFSV QNFLLPERDV
210 220 230 240 250
LPMHCAANIG EAGDVTLFFG LSGTGKTTLS ADESRYLIGD DEHGWGEGVV
260 270 280 290 300
FNVEGGCYAK CIDLSEKNEP VIWKAIKFGA VLENVVLDEE RVPNYADDSL
310 320 330 340 350
TQNSRAAYPL EHVEKRSEKN LGGEPNAVIF LTCDLTGVLP PVSILNNEQA
360 370 380 390 400
AYHFLSGYTA LVGSTEMGSG GGIKSTFSTC FGAPFFPRPA GVYAELLIKR
410 420 430 440 450
IKAFGSKVYL VNTGWTGGGY GVGKRFNIPT TRGVIAAIQS GALIGAETEH
460 470 480 490 500
LDIINLDVPK AVPGVETNLL NPRNTWADKA AYDEAAKGLA KQFIENFKKF
510
EVSDAIKAAG PQL
Length:513
Mass (Da):55,732
Last modified:February 10, 2009 - v1
Checksum:iBFE7F109710B124C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM209186 Genomic DNA. Translation: CAW30340.1.
RefSeqiYP_002443164.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW30340; CAW30340; PLES_55861.
GeneIDi7179271.
KEGGipag:PLES_55861.
PATRICi19821099. VBIPseAer113719_5741.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM209186 Genomic DNA. Translation: CAW30340.1 .
RefSeqi YP_002443164.1. NC_011770.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557722.PLES_55861.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAW30340 ; CAW30340 ; PLES_55861 .
GeneIDi 7179271.
KEGGi pag:PLES_55861.
PATRICi 19821099. VBIPseAer113719_5741.

Phylogenomic databases

eggNOGi COG1866.
HOGENOMi HOG000271471.
KOi K01610.
OMAi GADPEHY.
OrthoDBi EOG6DG2RK.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci PAER557722:GHJW-5677-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00453. PEPCK_ATP.
InterProi IPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view ]
Pfami PF01293. PEPCK_ATP. 1 hit.
[Graphical view ]
PIRSFi PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
TIGRFAMsi TIGR00224. pckA. 1 hit.
PROSITEi PS00532. PEPCK_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58.

Entry informationi

Entry nameiPCKA_PSEA8
AccessioniPrimary (citable) accession number: B7V3T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3