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B7V3T9 (PCKA_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [ATP]

Short name=PCK
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49
Gene names
Name:pckA
Ordered Locus Names:PLES_55861
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA By similarity. HAMAP-Rule MF_00453

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00453

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00453

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00453.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandATP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (ATP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453
PRO_1000125081

Regions

Nucleotide binding220 – 2289ATP By similarity

Sites

Metal binding1851Manganese By similarity
Metal binding2041Manganese; via tele nitrogen By similarity
Metal binding2411Manganese By similarity
Binding site451Substrate By similarity
Binding site1791Substrate By similarity
Binding site1851ATP By similarity
Binding site1851Substrate By similarity
Binding site2041ATP By similarity
Binding site2691ATP By similarity
Binding site3051ATP By similarity
Binding site3051Substrate By similarity
Binding site4311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7V3T9 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: BFE7F109710B124C

FASTA51355,732
        10         20         30         40         50         60 
MTQANNAVYT DISAAQLVEE AIRRGEGELA ANGSLVVRTG HRTGRSPVDR FIVEEPSTKD 

        70         80         90        100        110        120 
AIAWGNINRP FPADKFDALW ARVEAFNNAQ DHFISHVHVG SAEAYYLPVK MTTATAWQNL 

       130        140        150        160        170        180 
FGRCLFIEPE QYNPAGKDEW QVLNVANFEC VPERDGTNSD GCVILNFAQK KVLIAGMRYA 

       190        200        210        220        230        240 
GEMKKAMFSV QNFLLPERDV LPMHCAANIG EAGDVTLFFG LSGTGKTTLS ADESRYLIGD 

       250        260        270        280        290        300 
DEHGWGEGVV FNVEGGCYAK CIDLSEKNEP VIWKAIKFGA VLENVVLDEE RVPNYADDSL 

       310        320        330        340        350        360 
TQNSRAAYPL EHVEKRSEKN LGGEPNAVIF LTCDLTGVLP PVSILNNEQA AYHFLSGYTA 

       370        380        390        400        410        420 
LVGSTEMGSG GGIKSTFSTC FGAPFFPRPA GVYAELLIKR IKAFGSKVYL VNTGWTGGGY 

       430        440        450        460        470        480 
GVGKRFNIPT TRGVIAAIQS GALIGAETEH LDIINLDVPK AVPGVETNLL NPRNTWADKA 

       490        500        510 
AYDEAAKGLA KQFIENFKKF EVSDAIKAAG PQL 

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM209186 Genomic DNA. Translation: CAW30340.1.
RefSeqYP_002443164.1. NC_011770.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557722.PLES_55861.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW30340; CAW30340; PLES_55861.
GeneID7179271.
KEGGpag:PLES_55861.
PATRIC19821099. VBIPseAer113719_5741.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1866.
HOGENOMHOG000271471.
KOK01610.
OMARYAGEMK.
OrthoDBEOG6DG2RK.
ProtClustDBPRK09344.

Enzyme and pathway databases

BioCycPAER557722:GHJW-5677-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00453. PEPCK_ATP.
InterProIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
TIGRFAMsTIGR00224. pckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKA_PSEA8
AccessionPrimary (citable) accession number: B7V3T9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways