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B7V0L5 (ISPE_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:PLES_50551
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2822824-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061
PRO_1000116933

Regions

Nucleotide binding95 – 10511ATP Potential

Sites

Active site121 By similarity
Active site1371 By similarity

Sequences

Sequence LengthMass (Da)Tools
B7V0L5 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 141EEBCC6C9438D3

FASTA28230,829
        10         20         30         40         50         60 
MSVRLSLPAP AKLNLFLHIL GRRDDGYHEL QTLFQFLDHG DELHFEARQD GQVRLHTEIA 

        70         80         90        100        110        120 
GVPHDSNLIV RAARGLQEAS GSPQGVDIWL DKRLPMGGGI GGGSSDAATT LLALNHLWQL 

       130        140        150        160        170        180 
GWDEDRIAAL GLRLGADVPV FTRGRAAFAE GVGEKLTPVD IPEPWYLVVV PQVLVSTAEI 

       190        200        210        220        230        240 
FSDPLLTRDS PAIKVRTVLE GDSRNDCQPV VERRYPEVRN ALILLNKFVS ARLTGTGGCV 

       250        260        270        280 
FGSFPNKAEA DKVSALLPDH LQRFVAKGSN VSMLHRKLET LV 

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM209186 Genomic DNA. Translation: CAW29809.1.
RefSeqYP_002442635.1. NC_011770.1.

3D structure databases

ProteinModelPortalB7V0L5.
SMRB7V0L5. Positions 4-278.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557722.PLES_50551.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW29809; CAW29809; PLES_50551.
GeneID7178911.
KEGGpag:PLES_50551.
PATRIC19819971. VBIPseAer113719_5192.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHOG000019601.
KOK00919.
OMAVSTAEIF.
OrthoDBEOG62VNQ2.

Enzyme and pathway databases

BioCycPAER557722:GHJW-5127-MONOMER.
UniPathwayUPA00056; UER00094.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_PSEA8
AccessionPrimary (citable) accession number: B7V0L5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways