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B7V0L5

- ISPE_PSEA8

UniProt

B7V0L5 - ISPE_PSEA8

Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

    Catalytic activityi

    ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei12 – 121UniRule annotation
    Active sitei137 – 1371UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi95 – 10511ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
    2. terpenoid biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Isoprene biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPAER557722:GHJW-5127-MONOMER.
    UniPathwayiUPA00056; UER00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
    Short name:
    CMKUniRule annotation
    Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
    Gene namesi
    Name:ispEUniRule annotation
    Ordered Locus Names:PLES_50551
    OrganismiPseudomonas aeruginosa (strain LESB58)
    Taxonomic identifieri557722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000001527: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2822824-diphosphocytidyl-2-C-methyl-D-erythritol kinasePRO_1000116933Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi557722.PLES_50551.

    Structurei

    3D structure databases

    ProteinModelPortaliB7V0L5.
    SMRiB7V0L5. Positions 4-278.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1947.
    HOGENOMiHOG000019601.
    KOiK00919.
    OMAiVSTAEIF.
    OrthoDBiEOG62VNQ2.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00061. IspE.
    InterProiIPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR004424. IspE.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
    PfamiPF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF010376. IspE. 1 hit.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00154. ispE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7V0L5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVRLSLPAP AKLNLFLHIL GRRDDGYHEL QTLFQFLDHG DELHFEARQD    50
    GQVRLHTEIA GVPHDSNLIV RAARGLQEAS GSPQGVDIWL DKRLPMGGGI 100
    GGGSSDAATT LLALNHLWQL GWDEDRIAAL GLRLGADVPV FTRGRAAFAE 150
    GVGEKLTPVD IPEPWYLVVV PQVLVSTAEI FSDPLLTRDS PAIKVRTVLE 200
    GDSRNDCQPV VERRYPEVRN ALILLNKFVS ARLTGTGGCV FGSFPNKAEA 250
    DKVSALLPDH LQRFVAKGSN VSMLHRKLET LV 282
    Length:282
    Mass (Da):30,829
    Last modified:February 10, 2009 - v1
    Checksum:i141EEBCC6C9438D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM209186 Genomic DNA. Translation: CAW29809.1.
    RefSeqiYP_002442635.1. NC_011770.1.

    Genome annotation databases

    EnsemblBacteriaiCAW29809; CAW29809; PLES_50551.
    GeneIDi7178911.
    KEGGipag:PLES_50551.
    PATRICi19819971. VBIPseAer113719_5192.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM209186 Genomic DNA. Translation: CAW29809.1 .
    RefSeqi YP_002442635.1. NC_011770.1.

    3D structure databases

    ProteinModelPortali B7V0L5.
    SMRi B7V0L5. Positions 4-278.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 557722.PLES_50551.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAW29809 ; CAW29809 ; PLES_50551 .
    GeneIDi 7178911.
    KEGGi pag:PLES_50551.
    PATRICi 19819971. VBIPseAer113719_5192.

    Phylogenomic databases

    eggNOGi COG1947.
    HOGENOMi HOG000019601.
    KOi K00919.
    OMAi VSTAEIF.
    OrthoDBi EOG62VNQ2.

    Enzyme and pathway databases

    UniPathwayi UPA00056 ; UER00094 .
    BioCyci PAER557722:GHJW-5127-MONOMER.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPi MF_00061. IspE.
    InterProi IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR004424. IspE.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR20861:SF2. PTHR20861:SF2. 1 hit.
    Pfami PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF010376. IspE. 1 hit.
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsi TIGR00154. ispE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
      Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
      Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LESB58.

    Entry informationi

    Entry nameiISPE_PSEA8
    AccessioniPrimary (citable) accession number: B7V0L5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3