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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei567Aminoacyl-adenylateUniRule annotation1
Binding sitei611ATPUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PLES_49431
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891881 – 943Isoleucine--tRNA ligaseAdd BLAST943

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7V0A3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi608 – 612"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B7V0A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKATLNL PETAFPMKAG LPQREPETLK FWNDIGLYQK LRAIGGDRPK
60 70 80 90 100
FVLHDGPPYA NGSIHIGHAV NKILKDIIVR SKTLAGYDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVETTH GKNLPADKTR ELCREYAAEQ IEGQKADFIR LGVLGEWDNP
160 170 180 190 200
YKTMNFANEA NEIRALAEMV KQDFVFKGLK PVNWCFDCGS ALAEAEVEYA
210 220 230 240 250
DKKSPTIDVG FPVADADKLA AAFGLAALDK PAQIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPEIDYAL VDAGDRYLVL AEALVESCLA RYQREGKVVA TAKGEALELI
310 320 330 340 350
NFRHPFYERL SPVYLADYVA LDAGTGIVHS SPAYGEDDFY TCKRYGMSND
360 370 380 390 400
DILSPVQSNG VYVDSLPFFG GQFIWKANPN VVAKLEEVGS LLAHETINHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKQPRQG ASLRERALEA ITQTEFVPGW
460 470 480 490 500
GQARLHGMIA GRPDWCISRQ RNWGVPIPFF LHKASGELHP RTVELMEEVA
510 520 530 540 550
QRVEKEGIEA WFKLDAAELL GEDAAQYEKI NDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHRIGHASGP VADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRQLLTHGF
610 620 630 640 650
TVDESGRKMS KSLGNTVVPQ TVIDTLGADI LRLWVASTDY SGEIAVSQQI
660 670 680 690 700
LQRSADAYRR IRNTTRFLLS NLNGFDPATD LLPPQEMLAL DRWAVDRALL
710 720 730 740 750
LQREIEEAYR EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA
760 770 780 790 800
RRSCQTALFH IAEALVRWIA PILAFTAEEV WKFLPGERAE SVMLATWYDG
810 820 830 840 850
LSELPADVTL NRQYWEQVMA VKAAVNKELE NQRAAKAVGG NLQAEVTLYA
860 870 880 890 900
EDALQVQLAK LGNELRFVLI TSTATLAPLS AAPADAVDSE VAGLKLKVVK
910 920 930 940
STHAKCGRCW HHREDVGQHA AHPDLCGRCI ENIEGSGEVR HYA
Length:943
Mass (Da):105,452
Last modified:February 10, 2009 - v1
Checksum:i156B21B3F31BFB9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA Translation: CAW29697.1
RefSeqiWP_004352756.1, NC_011770.1

Genome annotation databases

EnsemblBacteriaiCAW29697; CAW29697; PLES_49431
KEGGipag:PLES_49431

Similar proteinsi

Entry informationi

Entry nameiSYI_PSEA8
AccessioniPrimary (citable) accession number: B7V0A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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