B7UYZ2 (UNG_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	PLES_45931

Organism

Pseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]

Taxonomic identifier

557722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_1000199791
Sites
	Active site	71	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7UYZ2 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 53F2B36FF0D883A7	FASTA	231	26,010
	10 20 30 40 50 60 MTDNDDRIKL EASWKEALRE EFDKPYMKQL GEFLRQEKAA GKVIFPPGPL IFNALNTTPL 70 80 90 100 110 120 ENVKVVIIGQ DPYHGPGQAH GLCFSVQPGV PTPPSLQNIY KELNRDLNIP IPNNGYLQRW 130 140 150 160 170 180 AEQGVLLLNT SLTVEQAKAG SHANAGWQPF TDRVIEVVNE RCERLVFLLW GSHAQSKQKL 190 200 210 220 230 IDPQRHLILK SAHPSPLSAY RGFLGNGHFS RTNKFLEQNG KTPIDWSLPD L

References

[1]

"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa." Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C. Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LESB58.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM209186 Genomic DNA. Translation: CAW29347.1.
RefSeq	YP_002442176.1. NC_011770.1.
3D structure databases
ProteinModelPortal	B7UYZ2.
SMR	B7UYZ2. Positions 13-228.
ModBase	Search...
Protein-protein interaction databases
STRING	557722.PLES_45931.
Proteomic databases
PRIDE	B7UYZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAW29347; CAW29347; PLES_45931.
GeneID	7178579.
KEGG	pag:PLES_45931.
PATRIC	19818986. VBIPseAer113719_4711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0692.
HOGENOM	HOG000229528.
KO	K03648.
OMA	AGKEIYP.
ProtClustDB	PRK05254.
Enzyme and pathway databases
BioCyc	PAER557722:GHJW-5980-MONOMER.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_PSEA8

Accession

Primary (citable) accession number: B7UYZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program