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Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides.UniRule annotation

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation

Pathway:ipeptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621SubstrateUniRule annotation
Binding sitei70 – 701SubstrateUniRule annotation
Binding sitei131 – 1311SubstrateUniRule annotation
Sitei172 – 1721Important for catalytic activityUniRule annotation
Active sitei174 – 1741Proton donor/acceptorUniRule annotation
Active sitei244 – 2441NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciPAER557722:GHJW-2088-MONOMER.
UniPathwayiUPA00544.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
Alternative name(s):
Beta-N-acetylhexosaminidaseUniRule annotation
N-acetyl-beta-glucosaminidaseUniRule annotation
Gene namesi
Name:nagZUniRule annotation
Ordered Locus Names:PLES_20571
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Beta-hexosaminidasePRO_1000121066Add
BLAST

Interactioni

Structurei

3D structure databases

ProteinModelPortaliB7UYS5.
SMRiB7UYS5. Positions 3-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1622Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000248526.
KOiK01207.
OMAiDMVLICN.
OrthoDBiEOG6BCT06.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7UYS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGSLMLDIG GTWLTAEDRQ ILRHPEVGGL IIFARNIEHP AQVRELCAAI
60 70 80 90 100
RAIRPDLLLA VDQEGGRVQR LRQGFVRLPA MRAIADNPNA EELAEHCGWL
110 120 130 140 150
MATEVQAVGL DLSFAPVLDL DHQRSAVVGS RAFEGDPERA ALLAGAFIRG
160 170 180 190 200
MHAAGMAATG KHFPGHGWAE ADSHVAIPED ARSLEEIRRS DLVPFARLAG
210 220 230 240 250
QLDALMPAHV IYPQVDPQPA GFSRRWLQEI LRGELKFDGV IFSDDLSMAG
260 270 280 290 300
AHVVGDAASR IEAALAAGCD MGLVCNDRAS AELALAALQR LKVTPPSRLQ
310 320 330
RMRGKGYANT DYRQQPRWLE ALSALRAAQL ID
Length:332
Mass (Da):36,101
Last modified:February 10, 2009 - v1
Checksum:iBA472E62B44A097A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW26784.1.
RefSeqiWP_003118092.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW26784; CAW26784; PLES_20571.
KEGGipag:PLES_20571.
PATRICi19813765. VBIPseAer113719_2129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW26784.1.
RefSeqiWP_003118092.1. NC_011770.1.

3D structure databases

ProteinModelPortaliB7UYS5.
SMRiB7UYS5. Positions 3-325.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiB7UYS5.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAW26784; CAW26784; PLES_20571.
KEGGipag:PLES_20571.
PATRICi19813765. VBIPseAer113719_2129.

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000248526.
KOiK01207.
OMAiDMVLICN.
OrthoDBiEOG6BCT06.

Enzyme and pathway databases

UniPathwayiUPA00544.
BioCyciPAER557722:GHJW-2088-MONOMER.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58.

Entry informationi

Entry nameiNAGZ_PSEA8
AccessioniPrimary (citable) accession number: B7UYS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.