Reviewed, UniProtKB/Swiss-Prot B7UYS5 (NAGZ_PSEA8)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	PLES_20571

Organism

Pseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]

Taxonomic identifier

557722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Beta-hexosaminidase HAMAP MF_00364		PRO_1000121066
Sites
	Active site	244	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7UYS5-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: BA472E62B44A097A	FASTA	332	36,101
	10 20 30 40 50 60 MQGSLMLDIG GTWLTAEDRQ ILRHPEVGGL IIFARNIEHP AQVRELCAAI RAIRPDLLLA 70 80 90 100 110 120 VDQEGGRVQR LRQGFVRLPA MRAIADNPNA EELAEHCGWL MATEVQAVGL DLSFAPVLDL 130 140 150 160 170 180 DHQRSAVVGS RAFEGDPERA ALLAGAFIRG MHAAGMAATG KHFPGHGWAE ADSHVAIPED 190 200 210 220 230 240 ARSLEEIRRS DLVPFARLAG QLDALMPAHV IYPQVDPQPA GFSRRWLQEI LRGELKFDGV 250 260 270 280 290 300 IFSDDLSMAG AHVVGDAASR IEAALAAGCD MGLVCNDRAS AELALAALQR LKVTPPSRLQ 310 320 330 RMRGKGYANT DYRQQPRWLE ALSALRAAQL ID

References

[1]

"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa." Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C. Genome Res. 19:12-23(2009) [PubMed: 19047519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM209186 Genomic DNA. Translation: CAW26784.1.
RefSeq	YP_002439660.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7180781.
GenomeReviews	Gene locus PLES_20571 in contig FM209186_GR.
KEGG	pag:PLES_20571.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00364. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_PSEA8

Accession

Primary (citable) accession number: B7UYS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)