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B7UYI7 (B7UYI7_PSEA8) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase HAMAP-Rule MF_00419
Short name=FGAM synthase HAMAP-Rule MF_00419
Short name=FGAMS HAMAP-Rule MF_00419
EC=6.3.5.3 HAMAP-Rule MF_00419
Alternative name(s):
Formylglycinamide ribotide amidotransferase HAMAP-Rule MF_00419
Formylglycinamide ribotide synthetase HAMAP-Rule MF_00419
Gene names
Name:purL HAMAP-Rule MF_00419 EMBL CAW25943.1
Ordered Locus Names:PLES_12161
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length1298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. SAAS SAAS010073 HAMAP-Rule MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. SAAS SAAS010073 HAMAP-Rule MF_00419

Subunit structure

Monomer By similarity. SAAS SAAS010073 HAMAP-Rule MF_00419

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00419.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain. SAAS SAAS010073 HAMAP-Rule MF_00419

In the N-terminal section; belongs to the FGAMS family. HAMAP-Rule MF_00419

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain1045 – 1298254Glutamine amidotransferase type-1 By similarity HAMAP-Rule MF_00419
Nucleotide binding305 – 31612ATP By similarity HAMAP-Rule MF_00419

Sites

Active site11381Nucleophile By similarity HAMAP-Rule MF_00419
Active site12631 By similarity HAMAP-Rule MF_00419
Active site12651 By similarity HAMAP-Rule MF_00419

Sequences

Sequence LengthMass (Da)Tools
B7UYI7 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: EAE3CA6DAC364B64

FASTA1,298140,675
        10         20         30         40         50         60 
MLILRGAPAL SAFRHGKLLE QLTQHVPAVT GLYAEFAHFA DVTGALTADE EQVLARLLKY 

        70         80         90        100        110        120 
GPSVPVQEPS GRLFLVVPRF GTISPWSSKA SDIARNCGLA KIDRLERGIA YYVQGELSES 

       130        140        150        160        170        180 
DAQQVAARLH DRMTQLVLDR LEGAAELFSH AQPRPLTAVD VLGGGRAALE KANVELGLAL 

       190        200        210        220        230        240 
AEDEIDYLLK SFGELGRNPH DVELMMFAQA NSEHCRHKIF NASWDIDGQA QDKSLFGMIK 

       250        260        270        280        290        300 
NTYEMNREGV LSAYKDNAAV IVGHVAGRFF PDPQTREYAA SREPVQILMK VETHNHPTAI 

       310        320        330        340        350        360 
APFPGASTGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLQI PGFEQPWEVP YGKPERIVTA 

       370        380        390        400        410        420 
LDIMIEGPLG GAAFNNEFGR PALTGYFRTF EQKIATPHGE EVRGYHKPIM LAGGMGNIRD 

       430        440        450        460        470        480 
EHVQKGEISV GAKLIVLGGP AMLIGLGGGA ASSMATGASS ADLDFASVQR DNPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG ERNPISFIHD VGAGGLSNAL PELINDGGRG GRFELRAVPN DEPGMSPLEI 

       550        560        570        580        590        600 
WCNESQERYV LSVDAADFET FKAICERERC PFAVVGEAIE QRQLTVADSH FDNKPVDMPL 

       610        620        630        640        650        660 
EVLLGKAPRM HRAVTREAEL GDDFDAAGLE LQESVERVLR HPAVASKSFL ITIGDRTITG 

       670        680        690        700        710        720 
LVARDQMVGP WQVPVADCAV TATSFDVYTG EAMAMGERTP LALLDAPASG RMAIGETVTN 

       730        740        750        760        770        780 
LAAARIGKLS DIKLSANWMA AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMK 

       790        800        810        820        830        840 
TRWQDNGEDK SVTSPVSLIV TGFAPVADVR QSLTPQLRLD KGETDLILID LGRGKNRLGG 

       850        860        870        880        890        900 
SILAQVHGKL GRAVPDVDDA EDLKAFFAVI QGLNADGHIL AYHDRSDGGL ITSVLEMAFA 

       910        920        930        940        950        960 
GHCGVELNLD ALADSREELA AVLFSEELGA VIQVREGATP EVLAQFSAAG LDDCVAVIGQ 

       970        980        990       1000       1010       1020 
PVNGYEINLN YNGETVYSAQ RRILQRIWSE TSYQIQRLRD NADCAEQEFD ALLDEDNPGL 

      1030       1040       1050       1060       1070       1080 
SIKLSYDVND DIAAPYIKKG VRPKVAILRE QGVNGQVEMA AAFDRAGFAA IDVHMSDILA 

      1090       1100       1110       1120       1130       1140 
GRVDLDAFKG LVACGGFSYG DVLGAGEGWA KSILFNARAR DGFQAFFARK DSFALGVCNG 

      1150       1160       1170       1180       1190       1200 
CQMMSNLHEL IPGTEFWPHF VRNRSEQFEA RVAMVQVQES SSIFLQGMAG SRLPIAIAHG 

      1210       1220       1230       1240       1250       1260 
EGHAEFESEE ALLEADLSGC VSLRFVDNHG KVTEAYPANP NGSPRGITGL SSRDGRVTIM 

      1270       1280       1290 
MPHPERVFRA VQNSWRPDDW QEDGGWLRMF RNARVWVD

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM209186 Genomic DNA. Translation: CAW25943.1.
RefSeqYP_002438822.1. NC_011770.1.

3D structure databases

ProteinModelPortalB7UYI7.
ModBaseSearch...

Protein-protein interaction databases

STRING557722.PLES_12161.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW25943; CAW25943; PLES_12161.
GeneID7176030.
KEGGpag:PLES_12161.
PATRIC19812012. VBIPseAer113719_1266.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHOG000261358.
KOK01952.
OMATVKAVGM.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycPAER557722:GHJW-5652-MONOMER.
UniPathwayUPA00074; UER00128.

Family and domain databases

HAMAPMF_00419. PurL_1.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7UYI7_PSEA8
AccessionPrimary (citable) accession number: B7UYI7
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info