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B7UX15 (NAPA_PSEA8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:PLES_41471
OrganismPseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]
Taxonomic identifier557722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 834805Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186368

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B7UX15 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: D162B07D6EF008CB

FASTA83493,484
        10         20         30         40         50         60 
MNLTRREFAK ANAAAIAAAA AGLPILVRAS NLVTEADVTS LVWNKAPCRF CGTGCSVMVA 

        70         80         90        100        110        120 
TRDGQVVATH GDIKAEVNRG INCVKGYFLS KIMYGSDRLT RPLLRMKDGK FDKQGEFQPI 

       130        140        150        160        170        180 
SWEQAFDIMA EKFKAALKAK GPESVGMFGS GQWTVWEGYA ANKLFKAGLR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVMGFM RSFGMDEPMG CYDDIEATDS FVLWGSNMAE MHPVLWSRVT DRRLSAPQVK 

       250        260        270        280        290        300 
VAVLSTFEHR SFELADLPMV FKPQTDLIIL NYIANHIIES GAVNRDFVER HVRFAHGAED 

       310        320        330        340        350        360 
IGYGLRPDDP LEKKAKNADK ANTWSDIDFK AFAEFVKPYT LERTARESGV PAERLKALAE 

       370        380        390        400        410        420 
LYADPKRKVV SFWTMGFNQH TRGVWANNLI YNIHLLTGKI SEPGNSPFSL TGQPSACGTA 

       430        440        450        460        470        480 
REVGTFSHRL PADLVVTNPK HRETAEKIWK VPAGTIQEKV GFHAVQQSRM LKDGVLNVYW 

       490        500        510        520        530        540 
TQVSNNMQAG PNVMQEVLPG WRNPDNFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAFGN 

       550        560        570        580        590        600 
AERRTQFWHQ LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPELK GKTLYDVLFR 

       610        620        630        640        650        660 
NGQVDRFPAS DLAKGYANDE VDAFGFYIQK GLFEEYAAFG RGHGHDLAPF DAYHEARGLR 

       670        680        690        700        710        720 
WPVVDGKETR WRYREGYDPY VSKGSGVQFY GYPDKKAIAF ALPYEPPAEA PDQDYPFWLA 

       730        740        750        760        770        780 
TGRVLEHWHT GSMTARVPEL YKAVPDALVY MHPEDARQLK LRRGSEVKVV SRRGEIRARV 

       790        800        810        820        830 
ETRGRNKPPQ GLVFVPFFDA NKLINKVTLD ATDPISKQTD YKKCAVRIEL LNLA 

« Hide

References

[1]"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LESB58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM209186 Genomic DNA. Translation: CAW28902.1.
RefSeqYP_002441731.1. NC_011770.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557722.PLES_41471.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAW28902; CAW28902; PLES_41471.
GeneID7180385.
KEGGpag:PLES_41471.
PATRIC19818096. VBIPseAer113719_4273.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMADAFGFYI.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycPAER557722:GHJW-4197-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PSEA8
AccessionPrimary (citable) accession number: B7UX15
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families