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Protein

Ribosomal RNA large subunit methyltransferase M

Gene

rlmM

Organism
Pseudomonas aeruginosa (strain LESB58)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytidine(2498) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(2498) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei184 – 1841S-adenosyl-L-methionineUniRule annotation
Binding sitei236 – 2361S-adenosyl-L-methionineUniRule annotation
Binding sitei256 – 2561S-adenosyl-L-methionineUniRule annotation
Binding sitei272 – 2721S-adenosyl-L-methionineUniRule annotation
Active sitei301 – 3011Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPAER557722:GHJW-3815-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase MUniRule annotation (EC:2.1.1.186UniRule annotation)
Alternative name(s):
23S rRNA (cytidine2498-2'-O)-methyltransferaseUniRule annotation
23S rRNA 2'-O-ribose methyltransferase RlmMUniRule annotation
Gene namesi
Name:rlmMUniRule annotation
Ordered Locus Names:PLES_37661
OrganismiPseudomonas aeruginosa (strain LESB58)
Taxonomic identifieri557722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Ribosomal RNA large subunit methyltransferase MPRO_1000201523Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7UVG0.
SMRiB7UVG0. Positions 1-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni217 – 2204S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. RlmM subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000247137.
KOiK06968.
OMAiVIFECYQ.
OrthoDBiEOG651SSR.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01551. 23SrRNA_methyltr_M.
InterProiIPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR011224. rRNA_MeTrfase_M.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF028774. UCP028774. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

B7UVG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLLMHCRP GFEGEVCAEI AEHAATLEIP GYAKSKPASA HVEFVCQDAD
60 70 80 90 100
GAERLMRRLR FADLIFPRQW ARGPGFIELP ESQRIEVLLA ELASYPVCGS
110 120 130 140 150
LWLEVLDTNA GKEVSTFCRK FEKPLRAALV KAGRLQEDPA LPRLLLTFRS
160 170 180 190 200
GREVFVGLAE PRNSALWPMG IPRLKFPREA PSRSTLKLEE AWHQFIPRSE
210 220 230 240 250
WDKRLAPDML AVDLGAAPGG WTWQLVNREM RVTAVDNGPM AENLMYSGLV
260 270 280 290 300
DHQKVDGYQY RPRQRVDWMV CDIVEKPART GALIETWIGE GLCREAVVNL
310 320 330 340 350
KLPMKQRYAE VRKILQRLRE SFDARGLKVA IGCKQLYHDR EEVTCHLRRL

ER
Length:352
Mass (Da):40,157
Last modified:February 10, 2009 - v1
Checksum:i3030D5ABF0513EBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW28493.1.
RefSeqiWP_003087345.1. NC_011770.1.

Genome annotation databases

EnsemblBacteriaiCAW28493; CAW28493; PLES_37661.
KEGGipag:PLES_37661.
PATRICi19817314. VBIPseAer113719_3882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM209186 Genomic DNA. Translation: CAW28493.1.
RefSeqiWP_003087345.1. NC_011770.1.

3D structure databases

ProteinModelPortaliB7UVG0.
SMRiB7UVG0. Positions 1-350.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAW28493; CAW28493; PLES_37661.
KEGGipag:PLES_37661.
PATRICi19817314. VBIPseAer113719_3882.

Phylogenomic databases

HOGENOMiHOG000247137.
KOiK06968.
OMAiVIFECYQ.
OrthoDBiEOG651SSR.

Enzyme and pathway databases

BioCyciPAER557722:GHJW-3815-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01551. 23SrRNA_methyltr_M.
InterProiIPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR011224. rRNA_MeTrfase_M.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF028774. UCP028774. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa."
    Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.
    Genome Res. 19:12-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LESB58.

Entry informationi

Entry nameiRLMM_PSEA8
AccessioniPrimary (citable) accession number: B7UVG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 11, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.