Reviewed, UniProtKB/Swiss-Prot B7UUU9 (ALGL_PSEA8)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PLES_14861

Organism

Pseudomonas aeruginosa (strain LESB58) [Complete proteome] [HAMAP]

Taxonomic identifier

557722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity.
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 367	340	Alginate lyase HAMAP MF_00557		PRO_1000129144

Sequences

	Sequence		Length	Mass (Da)
	B7UUU9-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: E55B131CEDA5D1F6	FASTA	367	40,886
	10 20 30 40 50 60 MKTSHLIRIA LPGALAAALL ASQVSQAADL VPPPGYYAAV GERKGNAGSC PAVPPPYTGS 70 80 90 100 110 120 LVFTSKYEGS DSARATLNVK AEKTFRSQIK DITDMERGAT KLVTQYMRSG RDGDLACALN 130 140 150 160 170 180 WMSTWARAGA LQSDDFNHTG KSMRKWALGS LSGAYMRLKF SSSRPLAAHA EQSREIEDWF 190 200 210 220 230 240 ARLGTQVVRD WSGLPLKKIN NHSYWAAWSV MSTAVVTNRR DLFDWAVSEF KVAANQVDEQ 250 260 270 280 290 300 GFLPNELKRR QRALAYHNYA LPPLAMIAAF AQVNGVDLRQ ENHGALQRLA ERVMKGVDDE 310 320 330 340 350 360 ETFEEKTGED QDMTDLKVDN KYAWLEPYCA LYRCEPKMLE AKKDREPFNS FRLGGEVTRV FSREGGS

References

[1]

"Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool epidemic strain of Pseudomonas aeruginosa." Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I., Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A., Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D., Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C. Genome Res. 19:12-23(2009) [PubMed: 19047519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM209186 Genomic DNA. Translation: CAW26214.1.
RefSeq	YP_002439090.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7179561.
GenomeReviews	Gene locus PLES_14861 in contig FM209186_GR.
KEGG	pag:PLES_14861.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00557. [Tree]
InterPro	IPR008397. Alginate_lyase. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEA8

Accession

Primary (citable) accession number: B7UUU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)