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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciECOL574521:GJAO-1096-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:E2348C_1062
OrganismiEscherichia coli O127:H6 (strain E2348/69 / EPEC)
Taxonomic identifieri574521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008205 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBPRO_0000402669Add
BLAST

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7UNZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.
OrthoDBiEOG64FKDG.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

B7UNZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTARPEA ITFDPQQTAL IVVDMQNAYA TPGGYLDLAG FDVSTTRPVI
60 70 80 90 100
ANIQTAVTAA RAAGMLIIWF QNGWDEQYVE AGGPGSPNYH KSNALKTMRN
110 120 130 140 150
QPLLQGKLLT KGSWDYQLVD ELMPQPGDIV LPKPRYSGFF NTPLDSILRS
160 170 180 190 200
RGIRHLVFTG IATNVCVEST LRDGFFLEYF GVVLEDATHQ AGPEFAQKAA
210 220 230
LFNIETFFGW VSDVETFCDA LSSTSFARIA
Length:230
Mass (Da):25,282
Last modified:February 10, 2009 - v1
Checksum:i69A5F399B3EE86F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM180568 Genomic DNA. Translation: CAS08610.1.
RefSeqiWP_001340067.1. NC_011601.1.

Genome annotation databases

EnsemblBacteriaiCAS08610; CAS08610; E2348C_1062.
KEGGiecg:E2348C_1062.
PATRICi18341104. VBIEscCol90278_1081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM180568 Genomic DNA. Translation: CAS08610.1.
RefSeqiWP_001340067.1. NC_011601.1.

3D structure databases

ProteinModelPortaliB7UNZ4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAS08610; CAS08610; E2348C_1062.
KEGGiecg:E2348C_1062.
PATRICi18341104. VBIEscCol90278_1081.

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.
OrthoDBiEOG64FKDG.

Enzyme and pathway databases

BioCyciECOL574521:GJAO-1096-MONOMER.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
    Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
    J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E2348/69 / EPEC.

Entry informationi

Entry nameiRUTB_ECO27
AccessioniPrimary (citable) accession number: B7UNZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: February 10, 2009
Last modified: November 11, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.