B7UKC9 (GLPE_ECO27) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase glpE
EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	E2348C_3669

Organism

Escherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

574521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_1000148872
Regions
	Domain	17 – 105	89	Rhodanese
Sites
	Active site	65	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7UKC9 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 03C72DEAC2A8F016	FASTA	108	12,054
	10 20 30 40 50 60 MDQFECINVA DAHQKLQEKE AVLVDIRDPQ SFAMGHAAQA FHLTNDTLGA FMRDNDFDTP 70 80 90 100 VMVMCYHGNS SKGAAQYLLQ QGYDVVYSID GGFEAWQRQF PAEVAYGA

References

[1]

"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69." Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G. J. Bacteriol. 191:347-354(2009) [PubMed: 18952797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E2348/69 / EPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM180568 Genomic DNA. Translation: CAS11217.1.
RefSeq	YP_002331136.1. NC_011601.1.
3D structure databases
ProteinModelPortal	B7UKC9.
SMR	B7UKC9. Positions 1-108.
ModBase	Search...
Protein-protein interaction databases
STRING	B7UKC9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000115481; EBESCP00000105247; EBESCG00000114565.
GeneID	7061698.
GenomeReviews	Gene locus E2348_C_3669 in contig FM180568_GR.
KEGG	ecg:E2348C_3669.
PATRIC	18346530. VBIEscCol90278_3750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010658.
HOGENOM	HBG743107.
OMA	FSAWHEA.
ProtClustDB	PRK00162.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. IPR023695. Thiosulf_sulfurTrfase. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
KO	K02439.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_ECO27

Accession

Primary (citable) accession number: B7UKC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program