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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotationSAAS annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431SubstrateUniRule annotation
Active sitei48 – 481NucleophileUniRule annotation
Binding sitei76 – 761SubstrateUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Binding sitei200 – 2001SubstrateUniRule annotation

GO - Molecular functioni

  1. pseudouridine synthase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: InterPro

GO - Biological processi

  1. RNA processing Source: InterPro
  2. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciECOL574521:GJAO-3573-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotationSAAS annotation (EC:5.4.99.25UniRule annotationSAAS annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotationImported
Ordered Locus Names:E2348C_3447Imported
OrganismiEscherichia coli O127:H6 (strain E2348/69 / EPEC)Imported
Taxonomic identifieri574521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000008205 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi574521.E2348C_3447.

Structurei

3D structure databases

ProteinModelPortaliB7UJ61.
SMRiB7UJ61. Positions 10-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

B7UJ61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPRRRGRD INGVLLLDKP QGMSSNDALQ KVKRIYNANR AGHTGALDPL
60 70 80 90 100
ATGMLPICLG EATKFSQYLL DSDKRYRVIA RLGQRTDTSD ADGQIVEERP
110 120 130 140 150
VTFSAEQLAA ALDTFRGDIE QIPSMYSALK YQGKKLYEYA RQGIEVPREA
160 170 180 190 200
RPITVYELLF IRHEGNELEL EIHCSKGTYI RTIIDDLGEK LGCGAHVIYL
210 220 230 240 250
RRLAVSKYPV ERMVTLEHLR ELVEQAEQQD IPAAELLDPL LMPMDSPASD
260 270 280 290 300
YPVVNLPLTS SVYFKNGNPV RTSGAPLEGL VRVTEGENGK FIGMGEIDDE
310
GRVAPRRLVV EYPA
Length:314
Mass (Da):35,087
Last modified:February 10, 2009 - v1
Checksum:iFEA46C01E0E8E7A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM180568 Genomic DNA. Translation: CAS10995.1.
RefSeqiYP_002330915.1. NC_011601.1.

Genome annotation databases

EnsemblBacteriaiCAS10995; CAS10995; E2348C_3447.
GeneIDi7061154.
KEGGiecg:E2348C_3447.
PATRICi18346058. VBIEscCol90278_3520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM180568 Genomic DNA. Translation: CAS10995.1.
RefSeqiYP_002330915.1. NC_011601.1.

3D structure databases

ProteinModelPortaliB7UJ61.
SMRiB7UJ61. Positions 10-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi574521.E2348C_3447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAS10995; CAS10995; E2348C_3447.
GeneIDi7061154.
KEGGiecg:E2348C_3447.
PATRICi18346058. VBIEscCol90278_3520.

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Enzyme and pathway databases

BioCyciECOL574521:GJAO-3573-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
    Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
    J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E2348/69 / EPECImported.

Entry informationi

Entry nameiB7UJ61_ECO27
AccessioniPrimary (citable) accession number: B7UJ61
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.