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B7UJ61

- B7UJ61_ECO27

UniProt

B7UJ61 - B7UJ61_ECO27

Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotationSAAS annotation

    Catalytic activityi

    tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431SubstrateUniRule annotation
    Active sitei48 – 481NucleophileUniRule annotation
    Binding sitei76 – 761SubstrateUniRule annotation
    Binding sitei179 – 1791SubstrateUniRule annotation
    Binding sitei200 – 2001SubstrateUniRule annotation

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: UniProtKB-HAMAP
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    IsomeraseUniRule annotationSAAS annotation

    Keywords - Biological processi

    tRNA processingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciECOL574521:GJAO-3573-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
    Alternative name(s):
    tRNA pseudouridine(55) synthaseUniRule annotation
    tRNA pseudouridylate synthaseUniRule annotation
    tRNA-uridine isomeraseUniRule annotation
    Gene namesi
    Name:truBUniRule annotationImported
    Ordered Locus Names:E2348C_3447Imported
    OrganismiEscherichia coli O127:H6 (strain E2348/69 / EPEC)Imported
    Taxonomic identifieri574521 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000008205: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi574521.E2348C_3447.

    Structurei

    3D structure databases

    ProteinModelPortaliB7UJ61.
    SMRiB7UJ61. Positions 10-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0130.
    HOGENOMiHOG000231223.
    KOiK03177.
    OMAiYELQFIR.
    OrthoDBiEOG6358D3.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    HAMAPiMF_01080. TruB_bact.
    InterProiIPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR015947. PUA-like_domain.
    IPR014780. tRNA_psdUridine_synth_TruB.
    IPR015240. tRNA_sdUridine_synth_fam1_C.
    [Graphical view]
    PANTHERiPTHR13767. PTHR13767. 1 hit.
    PfamiPF09157. TruB-C_2. 1 hit.
    PF01509. TruB_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR00431. TruB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7UJ61-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRPRRRGRD INGVLLLDKP QGMSSNDALQ KVKRIYNANR AGHTGALDPL    50
    ATGMLPICLG EATKFSQYLL DSDKRYRVIA RLGQRTDTSD ADGQIVEERP 100
    VTFSAEQLAA ALDTFRGDIE QIPSMYSALK YQGKKLYEYA RQGIEVPREA 150
    RPITVYELLF IRHEGNELEL EIHCSKGTYI RTIIDDLGEK LGCGAHVIYL 200
    RRLAVSKYPV ERMVTLEHLR ELVEQAEQQD IPAAELLDPL LMPMDSPASD 250
    YPVVNLPLTS SVYFKNGNPV RTSGAPLEGL VRVTEGENGK FIGMGEIDDE 300
    GRVAPRRLVV EYPA 314
    Length:314
    Mass (Da):35,087
    Last modified:February 10, 2009 - v1
    Checksum:iFEA46C01E0E8E7A5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM180568 Genomic DNA. Translation: CAS10995.1.
    RefSeqiYP_002330915.1. NC_011601.1.

    Genome annotation databases

    EnsemblBacteriaiCAS10995; CAS10995; E2348C_3447.
    GeneIDi7061154.
    KEGGiecg:E2348C_3447.
    PATRICi18346058. VBIEscCol90278_3520.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM180568 Genomic DNA. Translation: CAS10995.1 .
    RefSeqi YP_002330915.1. NC_011601.1.

    3D structure databases

    ProteinModelPortali B7UJ61.
    SMRi B7UJ61. Positions 10-311.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 574521.E2348C_3447.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAS10995 ; CAS10995 ; E2348C_3447 .
    GeneIDi 7061154.
    KEGGi ecg:E2348C_3447.
    PATRICi 18346058. VBIEscCol90278_3520.

    Phylogenomic databases

    eggNOGi COG0130.
    HOGENOMi HOG000231223.
    KOi K03177.
    OMAi YELQFIR.
    OrthoDBi EOG6358D3.

    Enzyme and pathway databases

    BioCyci ECOL574521:GJAO-3573-MONOMER.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    HAMAPi MF_01080. TruB_bact.
    InterProi IPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR015947. PUA-like_domain.
    IPR014780. tRNA_psdUridine_synth_TruB.
    IPR015240. tRNA_sdUridine_synth_fam1_C.
    [Graphical view ]
    PANTHERi PTHR13767. PTHR13767. 1 hit.
    Pfami PF09157. TruB-C_2. 1 hit.
    PF01509. TruB_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR00431. TruB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
      Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
      J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E2348/69 / EPECImported.

    Entry informationi

    Entry nameiB7UJ61_ECO27
    AccessioniPrimary (citable) accession number: B7UJ61
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 10, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3